Microparticles in Contact with Cells: From Carriers to Multifunctional Tissue Modulators

Neto, Mafalda D.; Oliveira, Mariana B.

doi:10.1016/j.tibtech.2019.02.008

Cited by 82 publications

(81 citation statements)

References 106 publications

(144 reference statements)

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“…Microparticles, i.e., structures ranging from 1 to 1000 µm, have for long been the platform of choice for various biomedical applications, with numerous reports exploring microcarrier formulations for focal delivery and controlled release of therapeutics, as injectable tissue‐defect fillers, as biosensing tools, or as cell‐expansion systems in static/dynamic in vitro cultures …”

Section: Cell–biomaterials Assembliesmentioning

confidence: 99%

“…This technological progress, combined with particles physicochemical versatility, have provided the foundation for also exploring microparticles as injectable cell‐encapsulating platforms and for in vitro disease modeling . More recent endeavors have focused on exploring microparticles as building blocks for modular bottom‐up tissue engineering by taking advantage of their role as orchestrators of cellular aggregation and of their bioinstructive cues for 3D microtissues maturation . We will focus on cell–particle surface interactions at the material–bio interface rather than cell encapsulation which will be further discussed in hydrogel‐based platforms.…”

Section: Cell–biomaterials Assembliesmentioning

confidence: 99%

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Advanced Bottom‐Up Engineering of Living Architectures

et al. 2019

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Bottom‐up tissue engineering is a promising approach for designing modular biomimetic structures that aim to recapitulate the intricate hierarchy and biofunctionality of native human tissues. In recent years, this field has seen exciting progress driven by an increasing knowledge of biological systems and their rational deconstruction into key core components. Relevant advances in the bottom‐up assembly of unitary living blocks toward the creation of higher order bioarchitectures based on multicellular‐rich structures or multicomponent cell–biomaterial synergies are described. An up‐to‐date critical overview of long‐term existing and rapidly emerging technologies for integrative bottom‐up tissue engineering is provided, including discussion of their practical challenges and required advances. It is envisioned that a combination of cell–biomaterial constructs with bioadaptable features and biospecific 3D designs will contribute to the development of more robust and functional humanized tissues for therapies and disease models, as well as tools for fundamental biological studies.

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Section: Cell–biomaterials Assembliesmentioning

confidence: 99%

Section: Cell–biomaterials Assembliesmentioning

confidence: 99%

Advanced Bottom‐Up Engineering of Living Architectures

et al. 2019

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“…Microspheres, as solid scaffolds, usually possess high compression moduli and large surface areas, which are favorable for cell adhesion and spreading. Moreover, a well-fabricated porous structure may allow sufficient cell migration and maximum cell-cell interactions [45]. Microgels are usually used as microcarriers through cell-encapsulation strategies.…”

Section: Advantages Limitations and Prospectivementioning

confidence: 99%

“…However, the highly hydrophilic microenvironments may also constrain the suspended cells to a round shape, which decreases the cellular activity and function. Moreover, the surrounding hydrogel matrix may impede cell spread and migration, resulting in fewer cell interactions [45]. Based on these features, microspheres are relatively more widely applied for purpose of large-scale cell expansion, while microgels are more suitable as injectable platforms for cell delivery and therapy.…”

Section: Advantages Limitations and Prospectivementioning

confidence: 99%

“…In recent years, the microcarrier-based approach of 3D cell culture has also opened a door for fabrication of engineered tissues, which is one of the cutting-edge topics in tissue engineering and regeneration medicine. Different engineered/artificial tissues have been developed from cell-laden microcarriers either as model tissues for pharmacological and pathological studies or for tissue regeneration [44][45][46][47].…”

Section: Introductionmentioning

confidence: 99%

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Biopolymer-Based Microcarriers for Three-Dimensional Cell Culture and Engineered Tissue Formation

Huang

Abdalla

Xiao

et al. 2020

IJMS

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The concept of three-dimensional (3D) cell culture has been proposed to maintain cellular morphology and function as in vivo. Among different approaches for 3D cell culture, microcarrier technology provides a promising tool for cell adhesion, proliferation, and cellular interactions in 3D space mimicking the in vivo microenvironment. In particular, microcarriers based on biopolymers have been widely investigated because of their superior biocompatibility and biodegradability. Moreover, through bottom-up assembly, microcarriers have opened a bright door for fabricating engineered tissues, which is one of the cutting-edge topics in tissue engineering and regeneration medicine. This review takes an in-depth look into the recent advancements of microcarriers based on biopolymers—especially polysaccharides such as chitosan, chitin, cellulose, hyaluronic acid, alginate, and laminarin—for 3D cell culture and the fabrication of engineered tissues based on them. The current limitations and potential strategies were also discussed to shed some light on future directions.

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