Microparticle sizing by dynamic light scattering in fresh‐frozen plasma

Abstract

Our data demonstrates that DLS offers a novel approach to assessing MP size and distribution, a technique that could be easily adopted as a means of assessing MPs within either FFP or other blood products.

New cationic vesicles prepared with double chain surfactants from arginine: Role of the hydrophobic group on the antimicrobial activity and cytotoxicity

“…The DLS unit was a Malvern Zeta Nanosizer, working at 632.8 nm and 25ºC. The scattering intensity was measured at a 173º angle to the incident beam [11]. At least 10 runs were performed for each sample.…”

Microparticles: Role in Haemostasis and Venous Thromboembolism

“…These methods claim to accurately measure particles in the nanoscale size range (Yuana). For example , one such nanoscale technique uses the brownian motion of these small particles to detect and measure them (Harrison, 2009). These methods are expensive, intensive to perform and not yet widely available (Lawrie, 2009).…”

MPs or ICs?

“…Individual cytokines that are induced by microbial components triggering Toll-like receptors (TLRs) were reported to be inhibited by HDAC inhibitors. [3][4][5] Yet, the extent of those inhibitory effects and possible functional consequences during infections were largely unknown.…”

“…Indeed, newer flow cytometers can now distinguish 100-and 300-nm beads. 4,5 However, these calibration beads have fundamentally different properties of forward and side scatter compared with biologic MPs, and the lower limit of detection of biologic MPs by flow cytometry is currently unclear. Despite these advances in analysis of MPs by flow cytometry, inherent limitations of this approach remain because most machines only analyze the larger MPs.…”

“…Dynamic light scattering is excellent for determining size and quantification of particles but provides no information on the properties of the particle. 4 In addition, it is more suited for analyzing homogeneous populations of particles rather than heterogeneous MP populations. Atomic force microscopy and electron microscopy allow for accurate determination of particle size and shape, but cannot be used for routine analysis of clinical samples.…”