Microparticle Release from Cell Lines and Its Anti-Influenza Activity

Jantaratrirat, Saharat; Boonarkart, Chompunuch; Ruangrung, Kanyarat; Suptawiwat, Ornpreya; Auewarakul, Prasert

doi:10.1089/vim.2017.0201

Cited by 10 publications

(7 citation statements)

References 30 publications

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“…It is highly desired to achieve a sharp contrast of labelling and low background for a better diagnosis in a clinical setting. A close assessment of the SNA staining histogram for both cells is found to be a heterogeneous labelling pattern, which is consistent with earlier report 70 . However, P4 showed comparatively less heterogeneous labelling to both cell lines, especially for A549.…”

Section: Discussionsupporting

confidence: 92%

Capturing sialyl-glycan via a site-selective installation of boronic acid repertoire in peptides

Chatterjee

Chowdhury

Saproo

et al. 2022

Preprint

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The broad spectrum of covalent binding modes and interactions has ‘magically’ popularized the submission of boronic acid-mediated chemistry into chemical biology and medicinal chemistry. Since borono peptide-based applications are emerging, simplistic methods for direct late-stage and site-selective installation of a versatile boronic acid (BA) repertoire onto peptides are desirable, given the limited chemical strategies. Here, we investigate the efficacy of thiol-ene click chemistry for installing functionally versatile BA derivatives on numerous bioactive, native peptides in a site-selective manner. The work emphasizes adaptable applications with BA-modified peptides, such as cyclization, conjugation, and biomolecule recognition. To this end, the click method enables us to nurture the sialyl-glycans binding aptitude of various BA-modified WGA peptides concurrently. The consequence reveals that the WGA peptide (in silico derived from wheat germ agglutinin), which shows a considerably low affinity to sialic acid, turns into a potent and selective binder in the attendance of a suitable BA probe to it. The synergistic recognition intensified the binding and profiling of sialyl-glycan on cancer cell lines compared with widely used lectin, Sambucus nigra.

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Section: Discussionsupporting

confidence: 92%

Capturing sialyl-glycan via a site-selective installation of boronic acid repertoire in peptides

Chatterjee

Chowdhury

Saproo

et al. 2022

Preprint

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“…It is unclear, what function then EV-based tetraspanins might have in the context of viral infection, and it might be speculated that cells use EVs to titrate away virus into membrane structures that are unable to provide replication and protein synthesis machineries for the virus. This is supported by the findings that EVs might have anti-influenza infection activity in vitro [73].…”

Section: Tetraspanins In Pathogen Infectionmentioning

confidence: 58%

Engineering of Surface Proteins in Extracellular Vesicles for Tissue-Specific Targeting

Vogt¹,

Stadlmayr²,

Grillari³

et al. 2019

Current Topics in Biochemical Engineering

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Extracellular vesicles (EVs) have in the recent decades gained an important stand as vehicles enabling cell-to-cell transport and communication. With the advanced development towards their clinical use and increasing versatility of potential applications, improving their tissue-specific targeting in order to enhance their functionality in drug delivery opened as a challenging engineering field. In the past, the question of specific intercellular contact has been addressed by decoration of the EV surface with agents able of specific target recognition. An attractive possibility here is the modification of strongly overexpressed EV surface marker proteins towards recognition of target cells. As these proteins are involved in a plethora of biological functions in EV biogenesis, cargo targeting and intercellular transfer, a minimal impact on protein architecture upon modifications is desirable, which would also increase the stability of the exosomal preparation intended for therapeutic use. This chapter focuses on the possibilities of engineering of the EV marker proteins towards antigen-recognition units broadly applicable to endow EVs with tissue-targeting functionality.

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“…Our findings support a novel model of VZV vasculopathy pathogenesis, with adventitial fibroblast transformation to myofibroblasts contributing to arterial remodeling by proliferation and migration and further adventitial cell-to-cell viral dissemination and endothelial activation mediated by VZV-MPs. MPs are important intercellular signaling mediators, 3,7 but few studies have demonstrated their role as mediators of infectious propagation. 8 Viral proteins detected in MPs could facilitate interaction on host cells and may also regulate tropism.…”

Section: Discussionmentioning

confidence: 99%

Microparticle-mediated VZV propagation and endothelial activation

et al. 2020

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ObjectiveVaricella zoster virus (VZV) can spread anterogradely and infect cerebral arteries causing VZV vasculopathy and arterial ischemic stroke. In this study, we tested the hypothesis that virus-infected cerebrovascular fibroblasts undergo phenotypic changes that promote vascular remodeling and facilitate virus transmission in an in vitro model of VZV vasculopathy. The aims of this project were therefore to examine the changes that virus-infected human brain adventitial vascular fibroblasts (HBVAFs) undergo in an in vitro model of VZV vasculopathy and to identify disease biomarkers relating to VZV-related vasculopathy.MethodsHBVAFs were infected with VZV, and their ability to migrate, proliferate, transdifferentiate, and interact with endothelial cells was studied with flow cytometry. Microparticles (MPs) released from these cells were isolated and imaged with transmission electron microscopy, and their protein content was analyzed with mass spectrometry. Circulating MP profiles were also studied in children with VZV and non-VZV vasculopathy and compared with controls.ResultsVZV-infected HBVAFs transdifferentiated into myofibroblasts with enhanced proliferative and migratory capacity. Interaction of VZV-infected HBVAFs with endothelial cells resulted in endothelial dysfunction. These effects were, in part, mediated by the release of MPs from VZV-infected HBVAFs. These MPs contained VZV virions that could transmit VZV to neighboring cells, highlighting a novel model of VZV cell-to-cell viral dissemination. MPs positive for VZV were significantly higher in children with VZV-related vasculopathy compared to children with non-VZV vasculopathy (p = 0.01) and controls (p = 0.007).ConclusionsVZV-infected HBVAFs promote vascular remodeling and facilitate virus transmission. These effects were mediated by the release of apoptotic MPs that could transmit VZV infection to neighboring cells through a Trojan horse means of productive viral infection. VZV+ MPs may represent a disease biomarker worthy of further study.

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Microparticle Release from Cell Lines and Its Anti-Influenza Activity

Cited by 10 publications

References 30 publications

Capturing sialyl-glycan via a site-selective installation of boronic acid repertoire in peptides

Capturing sialyl-glycan via a site-selective installation of boronic acid repertoire in peptides

Engineering of Surface Proteins in Extracellular Vesicles for Tissue-Specific Targeting

Microparticle-mediated VZV propagation and endothelial activation

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