Microparticle-enhanced nephelometric immunoassay of human plasminogen

Marchal, Émile; Montagne, P.; Cuilliere, M. L.; Béné, Marie‐Christine; Fauré, G

doi:10.1002/(sici)1098-2825(1996)10:2<85::aid-jcla5>3.0.co;2-k

Cited by 3 publications

(2 citation statements)

References 28 publications

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“…In the present study, urinary PKLK concentrations in patients with CKD were 5‐ to 10‐fold lower than plasmin(ogen) concentrations reported in a previous study with the same cohort . This difference is only partially explained by the lower plasma concentration of PKLK (50 μg mL −1 ) compared to plasminogen (100 μg mL −1 ) and therefore may be due to difference in sieving through the damaged glomeruli. An explanation could be the fact that PKLK forms a complex with high molecular weight kininogen that hinders glomerular filtration.…”

Section: Discussioncontrasting

confidence: 84%

Plasma kallikrein activates the epithelial sodium channel in vitro but is not essential for volume retention in nephrotic mice

et al. 2018

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Section: Discussioncontrasting

confidence: 84%

Plasma kallikrein activates the epithelial sodium channel in vitro but is not essential for volume retention in nephrotic mice

et al. 2018

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“…20 In recent years, the nephelometric determination has received much attention in the immunoassay. 21,22 To our knowledge, however, the use of nephelometry for determination of nucleic acids has not been reported so far. In this paper, our focus is to develop nephelometry as a sensitive and convenient technique for the determination of micro amounts of nucleic acids.…”

mentioning

confidence: 99%

Nephelometric determination of micro amounts of nucleic acids with protamine sulfate

Li²,

Tong³

1999

Analyst

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Nucleic acids can form large particle complexes with protamine sulfate by electrostatic forces, which results in strong light scattering. Based on this, a nephelometric method is described for sensitive and convenient determination of nucleic acids with protamine sulfate by using a common spectrofluorimeter. Maximum light scattering is produced in the range of pH 2.2-4.4 with the same excitation and emission wavelengths at 365 nm. Under optimal conditions, the calibration curves are linear in the range 0.05-60.0 micrograms cm-3 for nucleic acids. The corresponding detection limits are 12.5 ng cm-3 for calf thymus DNA, 9.0 ng cm-3 for fish sperm DNA, and 18.0 ng cm-3 for yeast RNA, respectively. Six synthetic samples are determined with satisfactory results. The relative standard deviation of five replicate measurements is 3.2% for 2.0 micrograms cm-3 calf thymus DNA.

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Candidate‐based proteomics in the search for biomarkers of cardiovascular disease

Anderson¹

2005

The Journal of Physiology

345

249

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The key concept of proteomics (looking at many proteins at once) opens new avenues in the search for clinically useful biomarkers of disease, treatment response and ageing. As the number of proteins that can be detected in plasma or serum (the primary clinical diagnostic samples) increases towards 1000, a paradoxical decline has occurred in the number of new protein markers approved for diagnostic use in clinical laboratories. This review explores the limitations of current proteomics protein discovery platforms, and proposes an alternative approach, applicable to a range of biological/physiological problems, in which quantitative mass spectrometric methods developed for analytical chemistry are employed to measure limited sets of candidate markers in large sets of clinical samples. A set of 177 candidate biomarker proteins with reported associations to cardiovascular disease and stroke are presented as a starting point for such a 'directed proteomics' approach.

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Microparticle-enhanced nephelometric immunoassay of human plasminogen

Cited by 3 publications

References 28 publications

Plasma kallikrein activates the epithelial sodium channel in vitro but is not essential for volume retention in nephrotic mice

Plasma kallikrein activates the epithelial sodium channel in vitro but is not essential for volume retention in nephrotic mice

Nephelometric determination of micro amounts of nucleic acids with protamine sulfate

Candidate‐based proteomics in the search for biomarkers of cardiovascular disease

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