Microparticle Delivery of Plasmid DNA to Mammalian Cells

A Novel Method for Polymer Coating of Plasmid DNA: Initial Investigations into the Use of Pulse Laser Deposition and Gene Delivery

“…The preliminary in vitro transfection assay is now fully operational although it is not possible to compare RLU values obtained from separate experiments. While PLGA has been used in a pDNA formulation (He et al, 2004;Hedley and Barman, 2004;Prabha and Labhasetwar, 2004), the conditions for the PLD procedure applied in this study have not been completely established. In addition, since the range of different PLGAs available vary (different molecular weights and varying ratios of lactic-to-glycolic acid), it may be possible to select a particular PLGA compound for pDNA formulation.…”

Immunization with a poly (lactide co-glycolide) encapsulated plasmid DNA expressing antigenic regions of HPV 16 and 18 results in an increase in the precursor frequency of T cells that respond to epitopes from HPV 16, 18, 6 and 11

“…Amolimogene consists of a plasmid DNA (pDNA) vector that is encapsulated within poly-lactide co-glycolide (PLG) microparticles ($2 micron mean diameter). Microparticle encapsulation increases the stability of the pDNA vaccine within the host by protecting the pDNA from enzymatic cleavage [14][15][16][17]. Once injected into the host, the particulate nature of the formulation will cause local inflammation to occur and the resulting influx of antigen presenting cells (APC) will phagocytose the microparticles [18].…”