Microorganisms recognition and quantification by lectin adsorptive affinity impedance

Gamella, María; Campuzano, Susana; Parrado, Concepción; Reviejo, A.J.; Pingarrón, José M.

doi:10.1016/j.talanta.2009.01.059

Cited by 68 publications

(48 citation statements)

References 26 publications

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“…Pingarrón and coworkers developed electrochemical impedance sensors for the detection of Escherichia coli ( E. coli ) using screen-printed Au electrodes [82]. Con A was added to sample solutions containing E. coli to form E. coli -Con A aggregates, which were then adsorbed to the electrode.…”

Section: Pathogenic Bacteria and Toxin Sensorsmentioning

confidence: 99%

Recent Progress in Lectin-Based Biosensors

Wang

Anzai

2015

Materials

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This article reviews recent progress in the development of lectin-based biosensors used for the determination of glucose, pathogenic bacteria and toxins, cancer cells, and lectins. Lectin proteins have been widely used for the construction of optical and electrochemical biosensors by exploiting the specific binding affinity to carbohydrates. Among lectin proteins, concanavalin A (Con A) is most frequently used for this purpose as glucose- and mannose-selective lectin. Con A is useful for immobilizing enzymes including glucose oxidase (GOx) and horseradish peroxidase (HRP) on the surface of a solid support to construct glucose and hydrogen peroxide sensors, because these enzymes are covered with intrinsic hydrocarbon chains. Con A-modified electrodes can be used as biosensors sensitive to glucose, cancer cells, and pathogenic bacteria covered with hydrocarbon chains. The target substrates are selectively adsorbed to the surface of Con A-modified electrodes through strong affinity of Con A to hydrocarbon chains. A recent topic in the development of lectin-based biosensors is a successful use of nanomaterials, such as metal nanoparticles and carbon nanotubes, for amplifying output signals of the sensors. In addition, lectin-based biosensors are useful for studying glycan expression on living cells.

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Section: Pathogenic Bacteria and Toxin Sensorsmentioning

confidence: 99%

Recent Progress in Lectin-Based Biosensors

Wang

Anzai

2015

Materials

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“…Such a recognition system is superior to antibody or nucleic acid based systems, since the latter systems always require a prior knowledge on the target and specific reagents, which become increasingly problematic when the identities of which are unknown [60]. Furthermore, the molecule size of lectins are much smaller than antibodies, thus they allow higher densities of carbohydrate-sensing elements leading to higher sensitivity and lower non-specific adsorption [61,62]. Finally, agglutination between the lectins and bacteria occurs quickly.…”

Section: Types Of Impedance Detection Techniques For Foodborne Pathogmentioning

confidence: 99%

New Trends in Impedimetric Biosensors for the Detection of Foodborne Pathogenic Bacteria

Wang

Ying

2012

Sensors

226

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The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security. In recent years impedimetric biosensors which integrate biological recognition technology and impedance have gained widespread application in the field of bacteria detection. This paper presents an overview on the progress and application of impedimetric biosensors for detection of foodborne pathogenic bacteria, particularly the new trends in the past few years, including the new specific bio-recognition elements such as bacteriophage and lectin, the use of nanomaterials and microfluidics techniques. The applications of these new materials or techniques have provided unprecedented opportunities for the development of high-performance impedance bacteria biosensors. The significant developments of impedimetric biosensors for bacteria detection in the last five years have been reviewed according to the classification of with or without specific bio-recognition element. In addition, some microfluidics systems, which were used in the construction of impedimetric biosensors to improve analytical performance, are introduced in this review.

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“…For example, two biotinylated lectins [agglutinin I, derived from the castor oil plant ( Ricinus communis ), and concanavalin A, extracted from the jack bean ( Canavalia ensiformis )] were conjugated to gold nanoparticles and used to detect E. coli and B. cereus , detecting as few as 10 3 cells per assay (Gao et al , 2010). Similarly, lectin-based screenprinted gold electrodes have been used for the impedimetric label-free detection of E. coli at cell concentrations ranging from 5.0 × 10 3 to 5.0 × 10 7 CFU/ml (Gamella et al , 2009).…”

Section: 34mentioning

confidence: 99%