Microorganisms of low-temperature heavy oil reservoirs (Russia) and their possible application for enhanced oil recovery

Назина, Т. Н.; Соколова, Д. Ш.; Бабич, Т. Л.; Семенова, Е. М.; Ershov, A. P.; Bidzhieva, S. Kh.; Borzenkov, I. A.; Полтараус, А. Б.; Khisametdinov, M. R.; Tourova, T. P.

doi:10.1134/s0026261717060121

Cited by 26 publications

(20 citation statements)

References 19 publications

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“…This made it possible to classify the oil from two out of four oilfields as heavy oil. The studied areas of the Arkhangelskoe and Romashkinskoe oilfields (Tatneft, Tatarstan) and Cheremukhovskoe and Vostochno-Anzirskoe oilfields (Ritek, Volgograd oblast) are exploited with water-flooding [23].…”

Section: Characteristics Of the Studied Heavy Oil Reservoirs And Sampmentioning

confidence: 99%

“…Cell numbers of the microorganisms of the main metabolic groups were determined by inoculation of ten-fold dilutions of the water samples into liquid media in triplicate, as described earlier [23]. Salinity of the studied water samples was taken into consideration when preparing the media.…”

Section: Media Composition and Cultures Used In The Studymentioning

confidence: 99%

“…Aerobic heavy oil degrading bacteria Gordonia amicalis strain 6-1 (=VKM Ac-2795D) and Rhodococcus erythropolis strain HO-KS22 (=VKM Ac-2807D) were isolated earlier from the Cheremukhovskoe and Vostochno-Anzirskoe oilfields (Russia), respectively [23]. Fermentative enrichment 7SA was obtained by inoculation of the medium with peptone and glucose with water injected into well 1010 of the Vostochno-Anzirskoe oilfield.…”

Section: Media Composition and Cultures Used In The Studymentioning

confidence: 99%

“…These results indicate that both microorganisms from the surface freshwater basins and the reservoir microbiota adapted to oilfield conditions are delivered into the oil reservoir with injection water (a mixture of surface fresh water and formation water after oil removal). Earlier analysis of the 16S rRNA genes of the microbial community from injection water 5600* of the Cheremukhovskoe oilfield revealed bacteria of the genera Dechloromonas (15%), Phenylobacterium (12%), hydrocarbon-oxidizing bacteria of the genera Acinetobacter (5%) and Rhodococcus (1.2%), and of sulfateand sulfur-reducing bacteria of the genera Desulfovibrio, Desulfomicrobium, and Desulfuromonas [23]. Denaturing gradient gel electrophoresis (DGGE) analysis of the mcrA genes in injection water 5600* revealed methanogens of the genera Methanospirillum, Methanobacterium, and Methanoregula; production water from sample 5464 revealed methanogens of the genera Methanosarcina, Methanoculleus, and unidentified Thermoplasmata.…”

Section: Phylogenetic Diversity Of Archaea and Bacteria In Injection mentioning

confidence: 99%

“…A number of aerobic organotrophic bacteria from the genera Rhodococcus, Gordonia, Nocardia, Pseudomonas, Bacillus, Salinicola, Chromohalobacter, and Cellulomonas were previously isolated from Russian heavy oil reservoirs [23]. These bacteria produced biosurfactants in the media with a variety of organic substrates (acetate, glucose, sucrose, ethanol, peptone, and glycerol), as well as in the medium with crude oil.…”

Section: Aerobic Bacteria Producing Biosurfactantsmentioning

confidence: 99%

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The Potential Application of Microorganisms for Sustainable Petroleum Recovery from Heavy Oil Reservoirs

et al. 2019

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A microbial enhanced oil recovery (MEOR) technique was tested at low-temperature heavy oil reservoirs (Russia). The bioaugmentation approach used is based on the introduction of hydrocarbon-oxidizing bacteria into the oilfield in combination with an injection of oxygen as a H 2 O 2 solution in order to initiate the first stage of hydrocarbon oxidation and of (NH 4 ) 2 HPO 4 as a source of biogenic elements. Before the pilot trials, the microorganisms of petroleum reservoirs were investigated by high-throughput sequencing, as well as by culture-base and radioisotope techniques. Molecular studies revealed the differences in microbial composition of the carbonate and terrigenous oil reservoirs and the communities of injection and formation water. Aerobic bacteria Rhodococcus erythropolis HO-KS22 and Gordonia amicalis 6-1 isolated from oilfields oxidized oil and produced biosurfactants. Fermentative enrichment and pure cultures produced considerable amounts of low fatty acids and alcohols from sacchariferous substrates. In core-flooding tests, 43.0-53.5% of additional heavy oil was displaced by aerobic bacteria, producing biosurfactants, and 13.4-45.5% of oil was displaced by fermentative bacteria, producing low fatty acids, alcohols, and gas. A total of 1250 t additional oil was recovered as a result of the application of an MEOR technique at the Cheremukhovskoe heavy oil reservoir and Vostochno-Anzirskoe reservoir with light conventional oil.

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Section: Characteristics Of the Studied Heavy Oil Reservoirs And Sampmentioning

confidence: 99%

Section: Media Composition and Cultures Used In The Studymentioning

confidence: 99%

Section: Media Composition and Cultures Used In The Studymentioning

confidence: 99%

Section: Phylogenetic Diversity Of Archaea and Bacteria In Injection mentioning

confidence: 99%

Section: Aerobic Bacteria Producing Biosurfactantsmentioning

confidence: 99%

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The Potential Application of Microorganisms for Sustainable Petroleum Recovery from Heavy Oil Reservoirs

et al. 2019

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Top‐down enrichment of oil field microbiomes to limit souring and control oil composition during extraction operations

et al. 2022

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Microbial processes sour oil, corrode equipment, and degrade hydrocarbons at an annual global cost to the oil and gas industry of nearly $2 billion. However, top-down control of these microbial processes can reduce their damage and enhance oil recovery. Here, we screened microbial communities from five oil wells in the Illinois basin and evaluated nutrient injection strategies to control metabolism and community composition. Molasses with molybdate supplementation stimulated gas and organic acid production while suppressing corrosive H 2 S formation in samples from two wells. These changes were accompanied with significant reshaping of the microbiome community. Simulations of field operations via a lab-scale mini-coreflood validated that oil well microbiomes can be engineered to inhibit deleterious H 2 S and shape oil hydrocarbon composition in situ. These pilot studies validate the economic potential and sustainability of top-down approaches for microbiome engineering to control microbes in oil extraction and enhance the economic viability of oil recovery.comprehensive 2D-gas chromatography, cultivation screening, microbial enhanced oil recovery, microbiome engineering, miniature coreflood, top-down design | BACKGROUNDFossil fuels are likely to remain a significant energy source for at least the next 30 years 1 as economies transition globally toward more renewable energy sources. 2 Extracting trapped oil from aging wells can not only increase the overall production of existing wells but also minimize the need to drill new wells and, ultimately, decrease the cost of extracting oil from existing reservoirs. 3 Expensive polymer and/or surfactant/polymer formulations that require large amounts of funding and research effort are used to extract oil not recovered by primary extraction techniques. 4 Additionally, native and exogenous microbes have been harnessed as a more cost-effective 5 alternative for secondary and tertiary oil recovery-an approach coined microbial enhanced oil recovery (MEOR). 6 However, microbial activity in oil wells, can also be disruptive to oil recovery and/or alter its composition. 6 For example, microbes can degrade and sour oil, and produce metabolites that corrode the well casing, flowlines, and pipelines. 7

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Bacillus subtilis‐based microbial enhanced oil recovery (MEOR) in polymer microfluidic chip

et al. 2022

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Microbial enhanced oil recovery (MEOR) is a tertiary oil recovery process that manipulates the microbial environment inside oil reservoirs to modify the physical/chemical properties of the reservoirs to enhance the oil recovery. Up to now, the detailed MEOR mechanism is still not entirely clear due to the multiple influence factors (e.g., pH, nutrients, temperature, porosity, and permeability) on microbial growth and reproduction, as well as the lack of understanding of microbial's influencing mechanism on the oil recovery process. In this study, a Bacillus subtilis-based MEOR process was conducted in a polymethyl methacrylate (PMMA)-based microfluidic device to mimic the MEOR process in the reservoir. The porous microstructure based on real sandstone slice images was fabricated with laser ablation on a PMMA substrate. Two different MEOR approaches were conducted in the PMMA-based microfluidics devices: the direct injection of displacing reagent (biosurfactant produced by bacteria) into the microfluidic chip for the oil recovery (ex-situ), and the incubation of bacteria solution inside the chip followed with brine flooding (in-situ). The result indicates the ex-situ MEOR process with B. subtilis can reach a recovery rate of 38.56%, while the in-situ MEOR process with B. subtilis reached a recovery rate of 40.27%. The proposed study provides a new tool for understanding the MEOR process, with advantages in visibility and accurate fluid control during the MEOR process.

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Microorganisms of low-temperature heavy oil reservoirs (Russia) and their possible application for enhanced oil recovery

Cited by 26 publications

References 19 publications

The Potential Application of Microorganisms for Sustainable Petroleum Recovery from Heavy Oil Reservoirs

The Potential Application of Microorganisms for Sustainable Petroleum Recovery from Heavy Oil Reservoirs

Top‐down enrichment of oil field microbiomes to limit souring and control oil composition during extraction operations

Bacillus subtilis‐based microbial enhanced oil recovery (MEOR) in polymer microfluidic chip

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