Microorganisms isolated from pulp chambers

Keudell, K. C.; Conte, Mario; Fujimoto, L. Miya; Ernest, Michael A.; Berry, Hugh G.

doi:10.1016/s0099-2399(76)80012-x

Cited by 38 publications

(12 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…infected pulpitis, necrotic pulps, infected root dentine and periapical lesions (Wittgow & Sabiston 1975, Keudell et al 1976, Zavistoski et al 1980, Hoshino 1985, Tronstad et al 1987, Kobayashi et al 1990, Hoshino et al 1992, Sato et al 1993). Around 10 3 bacteria per mg dentine occurred in infected root dentine (Ando & Hoshino 1990), and such numbers of bacteria were placed in the reservoir channels.…”

Section: Discussionmentioning

confidence: 99%

Bacterial eradication from root dentine by ultrasonic irrigation with sodium hypochlorite

Huque

Kota²,

Yamaga³

et al. 1998

Int Endodontic J

View full text Add to dashboard Cite

The study aimed to evaluate intracanal irrigation procedures in eradicating bacteria from surface, shallow and deep layers of root dentine using extracted human teeth. Artificial bacterial smear layer was successfully produced by rubbing a mixture of dental plaque and artificially decalcified dentine or carious dentine on root canal walls. The reservoir holes were 3.5 mm in depth, 1 mm in diameter prepared 1.5 mm apart and parallel to the root canals on the decrowned planes, in which five separate bacterial species were placed (Actinomyces israelii, Fusobacterium nucleatum, Propionibacterium acnes, Streptococcus mutans and Streptococcus sanguis). Bacterial eradication after irrigation of the prepared canals was determined by bacterial recovery (i) from the root canal surfaces and shallow layers where bacteria were smeared artificially and (ii) from deeper layers of root canal dentine reservoir holes. Ultrasonic irrigation with 5.5% and 12% NaOCl eradicated bacteria from artificial smear layer (P < 0.0001), whilst 12% NaOCl irrigation with a syringe was insufficient. Ultrasonic irrigation with water or 15% EDTA-failed to eradicate bacteria from smeared surfaces. Ultrasonic irrigation with 12% NaOCl killed A. israelii, F. nucleatum, P. acnes, S. mutans, and S. sanguis placed in reservoir channels, although for F. nucleatum, a very small number of bacteria remained in five samples out of 12. Ultrasonic irrigation with less concentrated NaOCl failed to eliminate bacteria completely from reservoir channels in most samples. Ultrasonic irrigation with 12% NaOCl appeared to eliminate bacteria efficiently from surface, shallow and deep layers of root dentine.

show abstract

Section: Discussionmentioning

confidence: 99%

Bacterial eradication from root dentine by ultrasonic irrigation with sodium hypochlorite

Huque

Kota²,

Yamaga³

et al. 1998

Int Endodontic J

View full text Add to dashboard Cite

show abstract

“…Relatively few species do so. The bacteria isolated from infected root canals are composed of a restricted group of the oral flora (5,19,20,45,52), Most of the species that have been found in infected root canals have also been identified in the periodontal poeket (31,32), Although the root eanal flora is not as complex as that of the periodonta! pocket, they are similar in that both contain a special and limited assortment of the oral flora.…”

mentioning

confidence: 99%

Associations between microbial species in dental root canal infections

Sundqvist¹

1992

Oral Microbiology and Immunology

359

274

View full text Add to dashboard Cite

The existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. Samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. The most frequent species were Fusobacterium nucleatum, Prevotella intermedia, Peptostreptococcus micros, Peptostreptococcus anaerobius, Eubacterium alactolyticum, Eubacterium lentum and Wolinella recta. An odds ratio system was used to calculate positive or negative associations between the isolated bacteria. Strong positive associations were found between F. nucleatum and P. micros, Porphyromonas endodontalis, Selenomonas sputigena and W. recta. There was also a positive association between P. intermedia and P. micros, P. anaerobius and the eubacteria. In general, species of streptococci, Propionibacterium propionica, Capnocytophaga ochracea and Veillonella parvula showed no or negative associations with the other bacteria. The results are consistent with the concept of a special and selective environment occurring in the root canal that is due, in part, to the cooperative as well as antagonistic nature of the relationships between bacteria in the root canal.

show abstract

“…Thus some species of Bacteroides appear to show time-dependent resistance to phagocytosis, and could conceivably interfere with phagocytosis against other bacteria. These properties are envisaged as virulence factors and may be important in many naturally occurring endodontic infections that yield significant numbers of black-pigmented bacteria as a part of the flora (Kantz & Henry 1974, Wittgow & Sabiston 1975, Keudell et al 1976, Tronstad etal. 1987, Zavistoski etal.…”

Section: Discussionmentioning

confidence: 99%

“…While tnicrobiological analyses of specimens taken from puipal and periapical infections have itnplicated a myriad of pathogenic agents (Seltzer 1988), each specimen contains a mixed population consisting of three or more bacterial species (Wittgow & Sabiston 1975), and on average five different species {Za vistoski et al 1980). Using techniques to exclude attnospheric oxygen in the sampling of individual infected sites and subsequently in the cultivation of the microbes (Berg & Nord 1973), a significant portion of the bacteria have been identified as anaerobes (Kantz & Henry 1974;Keudell et al 1976;Tronstad et al 1987). Apparently low oxygen levels exist Correspondence: Dr M. K. Wu, Department of Operative Dentistry and Endodontics, School of Stomatology, Beijing Medicai Univereity, Wei Gong Cun, Haidian District, Beijing, China.…”

Section: Introductionmentioning

confidence: 99%

Bactericidal effects of human neutrophils and sera on selected endodotic pathogenic bacteria in an anaerobic environment

Henry

Gutmann³

1990

Int Endodontic J

View full text Add to dashboard Cite

Phagocytosis by normal human neutrophils and the bactericidal activity of normal human pooled serum were measured under anaerobic conditions on six endodontic pathogenic bacterial species from the genera Actinomyces, Bacteroides, Fusobacterium, Peptostreptococcus and Streptococcus. The results revealed that Actinomyces viscosus was not killed either by human neutrophils or by 20 per cent serum; Peptostreptococcus anaerobius was not killed by either 20 per cent or 95 per cent serum; and Bacteroides intermedius was susceptible to both 20 per cent and 95 per cent serum, but was not killed by neutrophils after exposure for 20 minutes. The other species showed intermediate susceptibility patterns. Such results suggest that oral pathogens vary with regard to the bactericidal effects of these two host defense mechanisms, and that this variation may at least in part explain the relative quantities of microbes recovered in certain oral infections.

show abstract

Microorganisms isolated from pulp chambers

Cited by 38 publications

References 5 publications

Bacterial eradication from root dentine by ultrasonic irrigation with sodium hypochlorite

Bacterial eradication from root dentine by ultrasonic irrigation with sodium hypochlorite

Associations between microbial species in dental root canal infections

Bactericidal effects of human neutrophils and sera on selected endodotic pathogenic bacteria in an anaerobic environment

Contact Info

Product

Resources

About