Microorganisms in a Disposal Site for Liquid Radioactive Wastes and Their Influence on Radionuclides

Назина, Т. Н.; Luk’yanova, E. A.; Zakharova, Еlena V.; Konstantinova, L. I.; Kalmykov, Stepan N.; Полтараус, А. Б.; Zubkov, A. A.

doi:10.1080/01490451003719044

Cited by 32 publications

(15 citation statements)

References 28 publications

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“…We found that Gammaproteobacterium HK31.3 associated with the wells HK31 (see Gislason et al, 2010 for description) and P. reactans strain SR3 (Genbank ID GQ258636), share 100% identity over 927 bases at the level of their 16S RNA genes (GenBank accession number BankIt1496454 HK31.3 JQ071900). Pseudomonas strains is a common groundwater microbe frequently observed in repository environments (e.g., Nazina et al, 2006Nazina et al, , 2010. For most experiments, cells were first cultured to a stationary stage on a shaker at 25°C in the darkness using final 10% NB with the following composition: 0.1 g/L glucose, 1.5 g/L peptone, 0.6 g/L NaCl and 0.3 g/L yeast extract.…”

Section: Bacteria Culturesmentioning

confidence: 99%

Effect of the heterotrophic bacterium Pseudomonas reactans on olivine dissolution kinetics and implications for CO2 storage in basalts

Shirokova

Bénézeth

Pokrovsky

et al. 2012

Geochimica et Cosmochimica Acta

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Section: Bacteria Culturesmentioning

confidence: 99%

Effect of the heterotrophic bacterium Pseudomonas reactans on olivine dissolution kinetics and implications for CO2 storage in basalts

Shirokova

Bénézeth

Pokrovsky

et al. 2012

Geochimica et Cosmochimica Acta

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“…Molecular hydrogen, nitrogen, and methane produced by microorganisms were analyzed by gas chromatography using approaches described previously [14]. Sulfide was determined with dimethyl-p-phenylenediamine by a modified colorimetric method of Pachmayr [27].…”

Section: Methodsmentioning

confidence: 99%

“…Numbers of anaerobic denitrifying, fermenting, iron-and sulfate-reducing, and methanogenic prokaryotes were estimated using the MPN technique with two replicates for each tenfold dilution of groundwater samples as described previously [14]. Aerobic organotrophs were enumerated in the TEG medium containing the following (g L-1 distilled water): bacto-tryptone, 5.0; yeast extract, 2.5; glucose, 1.0; pH 7.0.…”

Section: Microbial Enumeration and Isolationmentioning

confidence: 99%

“…Sequences of the 16S rRNA genes both of the cultured members of the phyla Proteobacteria, Dehalococcoides, Actinobacteria, Acidobacteria, Firmicutes, Bacteroidetes, Planctomycetes and of uncultured bacteria of Candidate divisions OD1, OP3 and OP11 were revealed in clone libraries of microorganisms from groundwater of a disposal site for liquid radioactive wastes at the Siberian Chemical Complex (SCC, Tomsk-7, or Seversk, Russia) [14].…”

Section: Introductionmentioning

confidence: 99%

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Ultramicrobacteria from Nitrate- and Radionuclide-Contaminated Groundwater

et al. 2020

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The goal of the present work was to investigate the physicochemical and radiochemical conditions and the microbial diversity in groundwater collected near the Lake Karachai (Russia), which was formerly used for the disposal of liquid radioactive waste, to isolate the dominant bacteria, and to determine their taxonomy and the physiological characteristics responsible for their adaptation to this environment. Groundwater samples contained high concentrations of acetate, oxalate, nitrate, and sulfate, as well as radionuclides. High-throughput sequencing and analysis of the clone libraries revealed lower microbial diversity in the most strongly contaminated groundwater and a predominance of bacteria of the genera Polynucleobacter, Pusillimonas, Candidatus Pelagibacter, and of the candidate phylum Parcubacteria; these groups include species with an ultra small cell size. Archaeal sequences in the libraries belonged to ammonium oxidizers of the phylum Thaumarchaeota and methanogens of the phylum Euryarchaeota. Pure cultures of obligate and facultative ultramicrobacteria belonging to the genera Chryseobacterium, Microbacterium, Salinibacterium, Pusillimonas, Roseomonas, and Janibacter were isolated from water samples. In genomes of Pusillimonas and Roseomonas strains the genes associated with nitrate reduction, resistance to heavy metals and metalloids were revealed. Several isolates are able to participate in the geochemical process of nitrate conversion to N 2 using acetate; this results in decreasing redox potential, which in turn may stimulate radionuclide reduction and decrease radionuclide migration in groundwater.

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“…As a ubiquitous microbial representative in natural environments, Pseudomonas strains were detected in numerous uranium contaminated sites, e.g., from the uranium mining waste pile "Haberland" (Saxony, Germany) (Geissler and SelenskaPobell 2005), and from the mill tailings of Shiprock, New Mexico, USA (Radeva and Selenska-Pobell 2005). Pseudomonas fluorescens was even detected in deep subsurface horizons, e.g., used for disposal of low-and intermediatelevel radioactive liquid waste of the Siberian Chemical Complex in Russia (Nazina et al 2010) and from the groundwater of the unique research facility for geological disposal, the Äspö Hard Rock Laboratory (HRL) in Sweden (Pedersen 2005).…”

Section: Introductionmentioning

confidence: 98%

A spectroscopic study on U(VI) biomineralization in cultivated Pseudomonas fluorescens biofilms isolated from granitic aquifers

Krawczyk-Bärsch

Lütke

Moll

et al. 2014

Environ Sci Pollut Res

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The interaction between the Pseudomonas fluorescens biofilm and U(VI) were studied using extended X-ray absorption fine structure spectroscopy (EXAFS), and time-resolved laser fluorescence spectroscopy (TRLFS). In EXAFS studies, the formation of a stable uranyl phosphate mineral, similar to autunite (Ca[UO2]2[PO4]2•2-6H2O) or meta-autunite (Ca[UO2]2[PO4]2•10-12H2O) was observed. This is the first time such a biomineralization process has been observed in P. fluorescens. Biomineralization occurs due to phosphate release from the cellular polyphosphate, likely as a cell's response to the added uranium. It differs significantly from the biosorption process occurring in the planktonic cells of the same strain. TRLFS studies of the uranium-contaminated nutrient medium identified aqueous Ca2UO2(CO3)3 and UO2(CO3)3 (4-) species, which in contrast to the biomineralization in the P. fluorescens biofilm, may contribute to the transport and migration of U(VI). The obtained results reveal that biofilms of P. fluorescens may play an important role in predicting the transport behavior of uranium in the environment. They will also contribute to the improvement of remediation methods in uranium-contaminated sites.

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Microorganisms in a Disposal Site for Liquid Radioactive Wastes and Their Influence on Radionuclides

Cited by 32 publications

References 28 publications

Effect of the heterotrophic bacterium Pseudomonas reactans on olivine dissolution kinetics and implications for CO2 storage in basalts

Effect of the heterotrophic bacterium Pseudomonas reactans on olivine dissolution kinetics and implications for CO2 storage in basalts

Ultramicrobacteria from Nitrate- and Radionuclide-Contaminated Groundwater

A spectroscopic study on U(VI) biomineralization in cultivated Pseudomonas fluorescens biofilms isolated from granitic aquifers

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