Microorganisms Associated with Uranium Bioremediation in a High-Salinity Subsurface Sediment

Nevin, Kelly P.; Finneran, Kevin T.; Lovley, Derek R.

doi:10.1128/aem.69.6.3672-3675.2003

Cited by 96 publications

(70 citation statements)

References 15 publications

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“…Diversity and community function Microbial communities have been extensively characterized at uranium-contaminated sites (Abdelouas et al, 2000;Chang et al, 2001Chang et al, , 2005Elias et al, 2003;Nevin et al, 2003;Vrionis et al, 2005) including the OR-FRC (Yan et al, 2003;North et al, 2004;Fields et al, 2005Fields et al, , 2006Akob et al, 2007). In general, it is assumed that bacterial diversity will depend upon the degree of contamination and will change in structure and Partition of the total variation into six components: unexplained variation; 'pure' temporal variation; 'shared' variation between environment and time; 'pure' environmental variation; 'shared' variation between environment and space; and 'pure' spatial variation.…”

Section: Discussionmentioning

confidence: 99%

Bacterial community succession during in situ uranium bioremediation: spatial similarities along controlled flow paths

Hwang

Gentry

et al. 2008

The ISME Journal

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Bacterial community succession was investigated in a field-scale subsurface reactor formed by a series of wells that received weekly ethanol additions to re-circulating groundwater. Ethanol additions stimulated denitrification, metal reduction, sulfate reduction and U(VI) reduction to sparingly soluble U(IV). Clone libraries of SSU rRNA gene sequences from groundwater samples enabled tracking of spatial and temporal changes over a 1.5-year period. Analyses showed that the communities changed in a manner consistent with geochemical variations that occurred along temporal and spatial scales. Canonical correspondence analysis revealed that the levels of nitrate, uranium, sulfide, sulfate and ethanol were strongly correlated with particular bacterial populations. As sulfate and U(VI) levels declined, sequences representative of sulfate reducers and metal reducers were detected at high levels. Ultimately, sequences associated with sulfate-reducing populations predominated, and sulfate levels declined as U(VI) remained at low levels. When engineering controls were compared with the population variation through canonical ordination, changes could be related to dissolved oxygen control and ethanol addition. The data also indicated that the indigenous populations responded differently to stimulation for bioreduction; however, the two biostimulated communities became more similar after different transitions in an idiosyncratic manner. The strong associations between particular environmental variables and certain populations provide insight into the establishment of practical and successful remediation strategies in radionuclidecontaminated environments with respect to engineering controls and microbial ecology.

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Section: Discussionmentioning

confidence: 99%

Bacterial community succession during in situ uranium bioremediation: spatial similarities along controlled flow paths

Hwang

Gentry

et al. 2008

The ISME Journal

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“…Accordingly, U contaminated settings such as mine sites, mill tailings, and sites where nuclear weapons have been manufactured have been studied directly by field observations or indirectly in laboratory experiments in which sediment and water samples collected from the field have been used to simulate in situ biogeochemical processes. The laboratory amendment of field sediments with organic substrates as electron donors to stimulate anaerobic microbial respiration typically results in significant removal of U from the pore water within a short period (< 1 month) (Abdelouas et al, 1999;Abdelouas et al, 2000;Finneran et al, 2002a;Finneran et al, 2002b;Suzuki et al, 2002;Elias et al, 2003a;Nevin et al, 2003;Suzuki et al, 2003;North et al, 2004;Gu et al, 2005a;Senko et al, 2005b;Tokunaga et al, 2005;Wan et al, 2005). No U(VI) reduction has been observed in the abiological controls, which strongly suggests that U(VI) reduction is biologically mediated.…”

Section: Redox Transformations Of U In U-contaminated Settingsmentioning

confidence: 88%

“…Under sulfate rich conditions, sulfate reducing Desulfosporosinus spp. appear to be associated with the stimulated U(VI) reduction (Nevin et al, 2003;Suzuki et al, 2003). In slightly acidic sediments, Anaeromycobacter spp.…”

Section: Redox Transformations Of U In U-contaminated Settingsmentioning

confidence: 99%

“…In all examples, U(VI) reduction was accompanied by the complete depletion of O 2 and nitrate (Senko et al, 2005b). Microbial populations associated with the stimulated U(VI) reduction have been characterized in a number of studies using molecular biological techniques (Holmes et al, 2002;Nevin et al, 2003;Petrie et al, 2003;Suzuki et al, 2003;North et al, 2004;Vrionis et al, 2005). U(VI) reduction is correlated with the enrichment of microorganisms related to known Fe(III) and U(VI) reducing Geobacter spp.…”

Section: Redox Transformations Of U In U-contaminated Settingsmentioning

confidence: 99%

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Geomicrobiological factors that control uranium mobility in the environment: Update on recent advances in the bioremediation of uranium-contaminated sites

Suzuki

Suko

2006

Journal of Mineralogical and Petrological Sciences

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Understanding the behavior of uranium (U) in the environment is essential not only for the protection of aquifers from U contamination but also for predicting the fate of U and other actinides disposed of in deep geological settings. It has long been believed that the redox chemistry of U can be simply predicted by thermodynamics and that the development of a low redox potential is a sufficient condition for U reduction. However, recent studies have demonstrated that redox transformations of U are controlled by kinetic factors that are strongly influenced by microbial activity. Although abiological U oxidation proceeds efficiently under oxygenic conditions, abiological reduction of U is inhibited by the formation of negatively charged U(VI) CO 3 complexes that prevail in nature. Phylogenetically diverse microorganisms are capable of enzymatically reducing U(VI) CO 3 complexes to form U(IV) bearing minerals such as uraninite (UO 2+x ). The only abiological pathway currently known for the reduction of U(VI) CO 3 complexes involves the Fe(II) monohydroxo surface complex Fe III OFe II OH 0 . This complex is mainly produced by the microbial reduction of Fe(III) in natural systems. Thus, U(VI) reduction is controlled both directly and indirectly, at least in part, by microbial activity. Several mechanisms of U oxidation under anoxic conditions have been revealed recently by laboratory and field studies. U(IV) is abiologically oxidized by Fe(III) and Mn(IV) oxides. Microbial reduction of nitrate to molecular nitrogen, which occurs following the depletion of O 2 , produces nitrogen intermediates including nitrite (NO 2 -), nitrous oxide (NO), and nitric oxide (N 2 O). Although the nitrogen intermediates oxidize U(IV), poorly crystalline Fe(III) oxide minerals resulting from the oxidation of aqueous Fe(II) species by the nitrogen intermediates oxidize U(IV) more efficiently than the nitrogen intermediates alone. Remarkably, the formation of Ca U(VI) CO 3 complexes resulting from increased levels of Ca 2+ and/or HCO 3 -leads to the reoxidation of bioreduced U(IV) under reducing conditions. These geomicrobiological factors pose challenges in manipulating and/or predicting the mobility and fate of U in complex and heterogeneous environmental settings.

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“…This strain is a good candidate for the accelerated bioremediation of systems contaminated by high levels of cadmium Sharma et al 2000 Subsurface sediment Uranium Stimulation of dissimilatory metal reduction activity of an enrichment of microorganisms closely related to Pseudomonas and Desulfosporisinus sp. which promote the reductive precipitation of uranium Nevin et al 2003 Selenite polluted site Selenium Ralstonia metallidurans CH34 can reduce selenite to elemental red selenium, that is highly insoluble Roux et al 2001 Subsurface sediment Subsurface environments Uranium Biofilm of the sulfate reducing bacterium Desulfovibrio desulfuricans attached to hematite (a-Fe 2 O 3 ) surface can accumulates both U(VI) and U(IV) and thus can limit the transport of uranium in subsurface environments Neal et al 2004 substances. Research in bioremediation is now opening the virtually infinite possibilities inherent in enzymatic systems.…”

Section: Cadmiummentioning

confidence: 99%

Developments in Bioremediation of Soils and Sediments Polluted with Metals and Radionuclides – 1. Microbial Processes and Mechanisms Affecting Bioremediation of Metal Contamination and Influencing Metal Toxicity and Transport

Tabak

Lens

Hullebusch

et al. 2005

Rev Environ Sci Biotechnol

193

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Microorganisms Associated with Uranium Bioremediation in a High-Salinity Subsurface Sediment

Cited by 96 publications

References 15 publications

Bacterial community succession during in situ uranium bioremediation: spatial similarities along controlled flow paths

Bacterial community succession during in situ uranium bioremediation: spatial similarities along controlled flow paths

Geomicrobiological factors that control uranium mobility in the environment: Update on recent advances in the bioremediation of uranium-contaminated sites

Developments in Bioremediation of Soils and Sediments Polluted with Metals and Radionuclides – 1. Microbial Processes and Mechanisms Affecting Bioremediation of Metal Contamination and Influencing Metal Toxicity and Transport

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