Microorganismos aislados en pacientes con mediastinitis poscirugía cardiaca en un hospital de cardiología de la Ciudad de México

Jiménez-González, María del C.; Mejía-Aguirre, Berenice; Ascencio-Montiel, Iván de J.

doi:10.24875/gmm.22000163

Cited by 1 publication

(1 citation statement)

References 32 publications

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“…S. haemolyticus have been isolated from clinical samples [ 64 ] and S. hominis from joint prosthesis, periprosthetic tissue, joint fluids, and fluid sonication of the joint prosthesis of the hip and knee from Mexican patients in Guadalajara and Nuevo León [ 65 ]. S. hominis have also been isolated from the blood [ 66 ] of patients with surgical-site infections undergoing cardiovascular surgery through median sternotomy [ 67 ]. S. intermedius have been isolated from hepatic abscess in a patient from Mexico City [ 68 ].…”

Section: Discussionmentioning

confidence: 99%

Detection of mecA Genes in Hospital-Acquired MRSA and SOSA Strains Associated with Biofilm Formation

González-Vázquez,

Córdova-Espinoza,

Escamilla-Gutiérrez

et al. 2024

Pathogens

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Methicillin-resistant (MR) Staphylococcus aureus (SA) and others, except for Staphylococcus aureus (SOSA), are common in healthcare-associated infections. SOSA encompass largely coagulase-negative staphylococci, including coagulase-positive staphylococcal species. Biofilm formation is encoded by the icaADBC operon and is involved in virulence. mecA encodes an additional penicillin-binding protein (PBP), PBP2a, that avoids the arrival of β-lactams at the target, found in the staphylococcal cassette chromosome mec (SCCmec). This work aims to detect mecA, the bap gene, the icaADBC operon, and types of SCCmec associated to biofilm in MRSA and SOSA strains. A total of 46% (37/80) of the strains were S. aureus, 44% (35/80) S. epidermidis, 5% (4/80) S. haemolyticus, 2.5% (2/80) S. hominis, 1.25% (1/80) S. intermedius, and 1.25% (1/80) S. saprophyticus. A total of 85% were MR, of which 95.5% showed mecA and 86.7% β-lactamase producers; thus, Staphylococcus may have more than one resistance mechanism. Healthcare-associated infection strains codified type I-III genes of SCCmec; types IV and V were associated to community-acquired strains (CA). Type II prevailed in MRSA mecA strains and type II and III in MRSOSA (methicillin-resistant staphylococci other than Staphylococcus aureus). The operon icaADBC was found in 24% of SA and 14% of SOSA; probably the arrangement of the operon, fork formation, and mutations influenced the variation. Methicillin resistance was mainly mediated by the mecA gene; however, there may be other mechanisms that also participate, since biofilm production is related to genes of the icaADBC operon and methicillin resistance was not associated with biofilm production. Therefore, it is necessary to strengthen surveillance to prevent the spread of these outbreaks both in the nosocomial environment and in the community.

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