Micronutrients intake is associated with improved sperm DNA quality in older men

Schmid, Thomas E.; Eskenazi, Brenda; Marchetti, Francesco; Young, Suzanne; Weldon, Rosana H.; Baumgartner, Andreas; Anderson, Diana; Wyrobek, Andrew J.

doi:10.1016/j.fertnstert.2012.07.1126

Cited by 57 publications

(33 citation statements)

References 45 publications

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“…This contrasts with results obtained in other species, in which the use of cobalamin improved seminal parameters in humans and in bulls (BLAIR et al, 1968;BOXMEER et al, 2009;FURNASS, 1963;SCHMID et al, 2012;WATSON, 1962;WONG et al, 2002). This, perhaps, is due to the fact that the stallions used in this study were young, fertile stallions , with good semen quality.…”

Section: Discussioncontrasting

confidence: 98%

“…The administration of intramuscular injections of cobalamin for seven months improved the semen of two men with poor quality semen, probably due to heavy smoking and alcohol consumption (BLAIR et al, 1968). These cases confirm other studies evaluating the influence of cobalamin on the fertility of mammals, suggesting that cobalamin may be beneficial in the treatment of poor fertility in males (BLAIR et al, 1968;SCHMID et al, 2012).…”

Section: Discussionsupporting

confidence: 79%

“…The prolonged use of Catosal B12® is not known to affect spermatogenesis and male fertility. Studies in different species have shown the importance of cobalamin on spermatogenesis and its positive effects on seminal quality; however, no studies have been performed in stallions (BLAIR et al, 1968;BOXMEER et al, 2009;FURNASS, 1963;SCHMID et al, 2012;WATSON, 1962;WONG et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

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Effect of intramuscular injection of butafosfan and cobalamin on the quality of Fresh and Cooled Stallion Semen

et al. 2015

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The use of butafosfan in combination with cobalamin modulates many cellular metabolic functions in several species. Its use enhances productive and reproductive performance and reduces stress responses in animals. Despite all these attributes, so far there have been no controlled studies to evaluate the effects of butafosfan and cobalamin on the quality of stallion semen. The purpose of this study was to evaluate the action of butafosfan in combination with cobalamin on the quality of fresh and cooled stallion semen. Four healthy stallions were kept in the same place and under the same management conditions during the entire experiment. Stallions were randomly assigned to two treatment groups in a 2x2 crossover design. Group A stallions were treated with an intramuscular injection of butafosfan twice a week for 80 days, while group B did not receive any treatment. After that, both groups were not treated for another 80 days allowing a washout period for the treated group. Then, the groups were reversed, and group B was treated with butafosfan and group A acted as the control for another 80 days. Semen was collected twice a week, diluted in skim milk and evaluated for total sperm count, total and progressive sperm motility, membrane integrity (CFDA/PI staining) and membrane functionality (HOS test) at 0 and 24 hours after preservation at 5ºC. Data were analyzed by comparing the values obtained from the treated stallions between the 60 th and 84 th days of treatment and the values obtained from the same stallion during the control period. The ejaculates of the washout period and between days 1 and 63 of treatment were not considered. A total of 85 ejaculates were analyzed by one-way ANOVA. Means were compared by the Tukey test at the 5% level of significance. No significant differences were observed in fresh and cooled semen regarding the total sperm count, total motility, progressive motility, membrane integrity and membrane functionality in the treated and control groups respectively. No interactions were detected in the fresh and cooled semen. The use of butafosfan and cobalamin in the stallions did not affect the seminal quality of young and fertile stallions. It can be used routinely and safely in these stallions. However, contrary to observations in humans and in bulls, the use of cobalamin did not improve semen parameters in these stallions. ResumoA utilização de Butafosfan em combinação com cobalamina modula uma série de funções metabólicas celulares em várias espécies. Seu uso melhora o desempenho produtivo e reprodutivo e reduz as respostas ao estresse em animais. Apesar de todos esses atributos, até agora não houve estudos controlados para avaliar os efeitos da substância Butafosfan e cobalamina sobre a qualidade do sêmen de garanhões. O objetivo deste estudo foi avaliar a ação do Butafosfan em combinação com cobalamina sobre a qualidade do sêmen fresco e resfriado de garanhões. Quatro garanhões saudáveis foram mantidos no mesmo lugar e sob as mesmas condições de manejo durante todo o experimen...

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Section: Discussioncontrasting

confidence: 98%

Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

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Effect of intramuscular injection of butafosfan and cobalamin on the quality of Fresh and Cooled Stallion Semen

et al. 2015

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“…Several studies have shown an association between sperm motility and leukocyte Mahmoud et al [7] T/LH ↓ Young: 5.5 nmol/U Older: 2.5 nmol/U contamination in ejaculate, ROS production, lipid peroxidation, and sperm oxidative DNA damage [72,77,78]. Some studies have indicated higher levels of dsDNA breaks and higher DNA fragmentation index (DFI) in older men [79][80][81][82] (Table 4). An imbalance between antioxidant defence mechanisms and ROS production eventually results in poor quality DNA, lipids, and proteins in the course of aging [88].…”

Section: Free Radical Theory Of Aging and Apoptosismentioning

confidence: 98%

Effects of aging on the male reproductive system

Güneş

Hekim

Arslan

et al. 2016

J Assist Reprod Genet

187

118

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The study aims to discuss the effects of aging on the male reproductive system. A systematic review was performed using PubMed from 1980 to 2014. Aging is a natural process comprising of irreversible changes due to a myriad of endogenous and environmental factors at the level of all organs and systems. In modern life, as more couples choose to postpone having a child due to various socioeconomic reasons, research for understanding the effects of aging on the reproductive system has gained an increased importance. Paternal aging also causes genetic and epigenetic changes in spermatozoa, which impair male reproductive functions through their adverse effects on sperm quality and count as, well as, on sexual organs and the hypothalamic-pituitarygonadal axis. Hormone production, spermatogenesis, and testes undergo changes as a man ages. These small changes lead to decrease in both the quality and quantity of spermatozoa. The offspring of older fathers show high prevalence of genetic abnormalities, childhood cancers, and several neuropsychiatric disorders. In addition, the latest advances in assisted reproductive techniques give older men a chance to have a child even with poor semen parameters. Further studies should investigate the onset of gonadal senesce and its effects on aging men.

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“…Additionally, low seminal plasma zinc levels detected in most our patients also affects fertility, as zinc is an important micronutrient for DNA repair during spermatogenesis. In males, low zinc levels adversely affected fertilization ability that improved with increase in dietary zinc intake [5,13]. Since zinc deficiency is common in TDT [14] examining the effect of increase zinc intake in TDT men, even with apparent normal plasma levels may be warranted.…”

mentioning

confidence: 99%

Comment on “deferiprone versus deferoxamine in thalassemia intermedia: Results from a 5‐year long‐term Italian multicenter randomized clinical trial”

Saliba

Taher

2015

American J Hematol

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knowledge was not demonstrated before. High seminal plasma iron can lessen sperm motility to 10% of normal, thus significantly affecting reproductive potential [8]. Further, the elevated systemic NTBI and LPI and low seminal GSH in our patients, correlating with sperm motility, imply that iron-induced oxidative stress may have a major role in the subfertility often detected in TDT men, beyond the hypogonadism caused by iron in the pituitary gland. Indirect evidence for seminal oxidative stress in TDT men was suggested [9]. In nonthalassemia subfertile men low seminal GSH levels were present, while normal GSH levels were associated with better sperm qualities [10]. Our results of normal sperm DNA fragmentation, an important measure for fertilization rate, contradict previous findings of increased sperm DNA fragmentation in TDT men [1,11]. It is possible that the relative low iron burden and normal seminal GSH levels found in our three patients were protective against increase DNA fragmentation. If confirmed in a larger study, it could suggest better preserved sperm quality in patients with consistent good iron chelation habits. Alternatively, diminished sperm DNA integrity was attributed to the use of the iron chelator desferroxamine [11,12], while our three patients were not using this chelation agent. Additionally, low seminal plasma zinc levels detected in most our patients also affects fertility, as zinc is an important micronutrient for DNA repair during spermatogenesis. In males, low zinc levels adversely affected fertilization ability that improved with increase in dietary zinc intake [5,13]. Since zinc deficiency is common in TDT [14] examining the effect of increase zinc intake in TDT men, even with apparent normal plasma levels may be warranted.This study suggests that individuals with high iron burden have decreased pituitary volume and low gonadotropin levels; additionally their seminal environment has a detrimental effect on sperm vitality and quality. In patients with consistent adherence to iron chelation the pituitary size and fertility measures are better preserved. The value of serial measures of pituitary volume and iron concentration needs further study but may prove to have a better predictive value for fertility potential than gonadotropin levels. Aggressive iron reduction along with antioxidant and zinc supplementation could assist with fertility preservation. The use of antioxidants supplementation was shown to improve sperm qualities, though the overall effectiveness remains controversial [4,15]. Clarifying the risk thresholds of these causative factors in larger studies can assist in treatment interventions and fertility preservation.

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Micronutrients intake is associated with improved sperm DNA quality in older men

Cited by 57 publications

References 45 publications

Effect of intramuscular injection of butafosfan and cobalamin on the quality of Fresh and Cooled Stallion Semen

Effect of intramuscular injection of butafosfan and cobalamin on the quality of Fresh and Cooled Stallion Semen

Effects of aging on the male reproductive system

Comment on “deferiprone versus deferoxamine in thalassemia intermedia: Results from a 5‐year long‐term Italian multicenter randomized clinical trial”

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