Micronucleus test in tadpole erythrocytes: Trends in studies and new paths

Benvindo‐Souza, Marcelino; Oliveira, Eliane Andreia Santos; Assis, Rhayane Alves; Santos, Cirley Gomes Araújo dos; Borges, Rinneu Elias; Silva, Daniela de Melo e; Santos, Lia Raquel de Souza

doi:10.1016/j.chemosphere.2019.124910

Cited by 35 publications

(16 citation statements)

References 59 publications

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“…Micronuclei, APs, NBs, and BCs all reached significant levels in both L. luctator and P. cuvieri , in particular at the highest imidacloprid concentrations. The formation of micronuclei is related to failures in mitotic division 74 and may be triggered by the presence of nuclei with bubbles or cellular binucleation 75 , which results from the blockage of cytokinesis by abnormal cell division (reviewed by Benvindo-Souza et al 76 ). Apoptotic nuclei undergo nuclear disintegration without suffering any alteration of the cytoplasm 77 , which indicates cell death 76 and is often associated with neurological disorders 78 .…”

Section: Discussionmentioning

confidence: 99%

Toxicity and genotoxicity of imidacloprid in the tadpoles of Leptodactylus luctator and Physalaemus cuvieri (Anura: Leptodactylidae)

Samojeden

Pavan

Rutkoski

et al. 2022

Sci Rep

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Imidacloprid is a neonicotinoid insecticide used to control agricultural pests around the world. This pesticide can have adverse effects on non-target organisms, especially in aquatic environments. The present study evaluated the toxicity of an imidacloprid-based insecticide in amphibians, using Leptodactylus luctator and Physalaemus cuvieri tadpoles as study models. Spawning of both species were collected within less than 24 h of oviposition from a non-agricultural land at Erechim, Rio Grande do Sul state, Brazil. Survival, swimming activity, body size, morphological malformations, and genotoxic parameters were analyzed at laboratory conditions. A short-term assay was conducted over 168 h (7 days) with five different concentrations of imidacloprid (3–300 µg L−1) being tested. The insecticide did not affect survival, although the tadpoles of both species presented reduced body size, malformed oral and intestine structures, and micronuclei and other erythrocyte nuclear abnormalities following exposure to this imidacloprid-based compound. Exposure also affected swimming activity in L. luctator, which reflected the greater sensitivity of L. luctator to imidacloprid in comparison with P. cuvieri. The swimming activity, body size, and malformations observed in L. luctator and the morphological malformations found in P. cuvieri indicated that even the lowest tested concentration of the insecticide were harmful to amphibians. At concentrations of over 3 μg L−1, P. cuvieri presents a smaller body size, and both species are affected by genotoxic cell damage. This demonstrates that imidacloprid is potentially toxic for the two study species at environmentally relevant concentrations.

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Section: Discussionmentioning

confidence: 99%

Toxicity and genotoxicity of imidacloprid in the tadpoles of Leptodactylus luctator and Physalaemus cuvieri (Anura: Leptodactylidae)

Samojeden

Pavan

Rutkoski

et al. 2022

Sci Rep

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“…MN is one of the most used methods to detect the early level of genetic damage induced by clastogenic or aneugenic agents in sentinel organisms exposed to environmental contaminants ( Fenech et al., 2016 ). Besides, other NAs are indicative of adverse cellular reactions and/or control mechanisms used to eliminate cells with damaged DNA, due to toxico-kinetic of the contaminant, hematopoietic cycle speed or incorrect or inefficient DNA repair, and have been interpreted as nuclear lesions analogous to MN ( Pollo et al., 2019 ; Benvindo-Souza et al., 2020 ). In the present work, there were a significant increase in the FMN and other FNAs (buds, NN, TNAs and EN) except for the BiN, in almost all exposed groups, respect to the NC.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we also registered a significant difference in the FMN between the NC and all the groups of neonates exposed in vivo to the same commercial formulations of the herbicide GLY (RU 11 mg/L and 2.5 mg/L), showing a concentration-dependent effect ( López González et al., 2013 ). Several studies have indicated an increase in MN and other NAs in different species exposed to pesticides ( Bonifacio and Hued, 2019 ; Pollo et al., 2019 ; Benvindo-Souza et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…The analysis of organisms in the natural environments provides information on the environmental health, generating data that are toxicologically relevant ( Marques et al., 2013 ). In contrast, it is difficult to establish a direct cause-and-effect relationship with a specific element because of environmental complexity under certain natural conditions and the interactions between substances ( Benvindo-Souza et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Besides, the description of other NAs in erythrocytes of caimans ( López González et al., 2017 ) has become, together with the MN frequencies and the CA, a common procedure for genotoxicity assessment. These abnormalities in the nucleus have been successfully applied in erythrocytes of many vertebrate species exposed to different genotoxic agents and considered in some cases analogous to MN formation ( Fenech et al., 2016 ; Schaumburg et al., 2016 ; Bonifacio and Hued et al., 2019 ; Pollo et al., 2019 ; Benvindo-Souza et al., 2020 ). LPO is probably the most studied ROS-induced process, which affects structures rich in polyunsaturated fatty acids, while oxidation of bases (purines and pyrimidine) is one of the most common types of DNA damage caused by ROS ( Ázqueta et al., 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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A comprehensive approach using multiple biomarkers to detect damage induced by pesticides in broad-snouted caiman (Caiman latirostris) under ex-situ conditions

González

Odetti²,

Latorre³

et al. 2022

Heliyon

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Caiman latirostris is one of the two species of the order Crocodylia that inhabit Argentina and is considered a species of vital ecological and economic importance in the north-east of Argentina. In this region, pesticides are the most common contaminants in natural environments and wild caiman populations are subject to this contamination constantly. The aim of this study was to evaluate the effects the main pesticides used in the region: glyphosate (GLY), cypermethrin (CYP) and chlorpyrifos (CPF) -based formulations, as well as the mixture of them, on C. latirostris juveniles under semi-controlled condition of exposure ( ex-situ ) during 75 days. One hundred yearling caimans (10-month-old) were equally distributed into five experimental groups (20 animals per group): a negative control (NC -tap water), GLY 2% (Roundup® Full II formulation -RU), CYP 0.12% (Atanor® formulation), CPF 0.8% (Lorsban® formulation), and a mixture of the three pesticides (Mx3: GLY 2% + CYP 0.12% + CPF 0.8%). We applied early warning biomarkers to detect damage induced by these chemicals in peripheral blood: activity of the antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD), analysis of lipid peroxidation (LPO) by the thiobarbituric acid reactive substances (TBARS), DNA damage and specific base oxidation through the standard and modified comet assay (CA), chromosome damage by micronucleus (MN) test and other nuclear abnormalities (NAs), hematological and growth parameters. Results showed a statistically significant increase in MN and NAs frequency, DNA damage, with an important contribution of base oxidation for all exposed groups compared to the NC. Total white blood cells count (TWBCC), and growth parameters showed effects mainly at the Mx3. The principal component analysis (PCA) demonstrated more sensitivity for biomarkers associated to genetic damage, including base oxidation to DNA than LPO, antioxidant enzyme modulation, immunotoxicity or growth parameters, to detect pesticides effects, applied under conditions similar to that found in natural environments.

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The Use of Multiple Biomarkers to Assess the Health of Anuran Amphibians in the Brazilian Cerrado Savanna: An Ecotoxicological Approach

Lopes

Benvindo‐Souza

Sotero

et al. 2023

Enviro Toxic and Chemistry

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Changes in the natural landscape and the indiscriminate use of pesticides can have a major impact on aquatic environments and have contributed to the worldwide decline of amphibian populations. In the present study, we sampled tadpoles of three anuran amphibians (Boana albopunctata, Physalaemus cuvieri, and Dendropsophus minutus) from ponds in six different agricultural landscapes of the Brazilian Cerrado savanna and evaluated whether and to what extent genotoxic and mutagenic damage was related to land use (the amount of forest and agricultural remnants, and related physicochemical factors) and the presence of pesticides in the water of the study ponds. We also evaluated the hepatotoxicity in P. cuvieri, which was the most abundant species at five of the six sampling points. Clomazone and Atrazine were the most common pesticides found in the ponds. The B. albopunctata and P. cuvieri tadpoles presented similar patterns of DNA damage among the sampling points. The least DNA damage was found in the D. minutus tadpoles, although this species was present in only one of the study ponds. More binucleated and anucleated cells were observed in B. albopunctata, but there was no significant variation among species in terms of the number of micronuclei or other erythrocytic nuclear abnormalities. Land use and physicochemical factors did not explain the variation in the DNA damage observed in the three anurans. The hepatotoxicity analyses of P. cuvieri revealed the presence of a series of alterations, including the enlargement of the sinusoids, vacuolization of the hepatocytes, the infiltration of inflammatory cells, hepatic steatosis, and dilation of the blood vessels. The interaction between physicochemical factors and the biomarkers analyzed in this study is complex. In particular, it will be important to better elucidate which factors are contributing, either directly or indirectly, to the decline of anuran amphibian populations, especially in threatened biomes, such the the Brazilian Cerrado. In this case, we would encourage further in situ studies that assess the ecotoxicology of the landscape, together with the systematic monitoring of aquatic environments, in order to guarantee the long‐term integrity of amphibian populations, and those of other organisms that play an essential functional role in the ecosystem.

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Micronucleus test in tadpole erythrocytes: Trends in studies and new paths

Cited by 35 publications

References 59 publications

Toxicity and genotoxicity of imidacloprid in the tadpoles of Leptodactylus luctator and Physalaemus cuvieri (Anura: Leptodactylidae)

Toxicity and genotoxicity of imidacloprid in the tadpoles of Leptodactylus luctator and Physalaemus cuvieri (Anura: Leptodactylidae)

A comprehensive approach using multiple biomarkers to detect damage induced by pesticides in broad-snouted caiman (Caiman latirostris) under ex-situ conditions

The Use of Multiple Biomarkers to Assess the Health of Anuran Amphibians in the Brazilian Cerrado Savanna: An Ecotoxicological Approach

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