Micronucleus frequencies and clonogenic cell survival in TK6 cells exposed to changing dose rates under controlled temperature conditions

Brehwens, Karl; Bajinskis, Ainars; Haghdoost, Siamak; Wójcik, Andrzej

doi:10.3109/09553002.2014.873831

Cited by 4 publications

(2 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is not the rule, however. Other studies have demonstrated greater effectiveness of lower dose rates [ 18 – 21 , 25 ]. To fully clarify this ambiguity, it is necessary to perform additional studies examining in depth signaling pathways in cells irradiated with dose rates commonly used in cancer radiotherapy.…”

Section: Discussionmentioning

confidence: 99%

“…Such an impact of dose rate was seen for doses exceeding 200 cGy [ 20 ]. Yet another study, performed on TK6 human lymphoblasts exposed to increased or decreased dose rates (0.22–10.6 cGy/min) demonstrated that the decreased dose rates were more effective in inducing micronuclei formation [ 21 ]. These researchers interpreted their results as the effect of interplay between DNA damage recognition and repair systems under changing dose rate conditions.…”

Section: Effect Of Dose Rate On Dna Damagementioning

confidence: 99%

See 1 more Smart Citation

Can high dose rates used in cancer radiotherapy change therapeutic effectiveness?

Konopacka¹,

Rogoliński²,

Sochanik³

et al. 2016

View full text Add to dashboard Cite

Current cancer radiotherapy relies on increasingly high dose rates of ionising radiation (100–2400 cGy/min). It is possible that changing dose rates is not paralleled by treatment effectiveness.Irradiating cancer cells is assumed to induce molecular alterations that ultimately lead to apoptotic death. Studies comparing the efficacy of radiation-induced DNA damage and apoptotic death in relation to varying dose rates do not provide unequivocal data. Whereas some have demonstrated higher dose rates (single dose) to effectively kill cancer cells, others claim the opposite. Recent gene expression studies in cells subject to variable dose rates stress alterations in molecular signalling, especially in the expression of genes linked to cell survival, immune response, and tumour progression. Novel irradiation techniques of modern cancer treatment do not rely anymore on maintaining absolute constancy of dose rates during radiation emission: instead, timing and exposure areas are regulated temporally and spatially by modulating the dose rate and beam shape. Such conditions may be reflected in tumour cells’ response to irradiation, and this is supported by the references provided.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Effect Of Dose Rate On Dna Damagementioning

confidence: 99%

Can high dose rates used in cancer radiotherapy change therapeutic effectiveness?

Konopacka¹,

Rogoliński²,

Sochanik³

et al. 2016

View full text Add to dashboard Cite

show abstract

Translocation dose-response curve for 137Cs γ-rays: Dose validation at various dose rate and changing dose rate conditions

Amula

Venkatraman

et al. 2021

Mutation Research/Genetic Toxicology and Environmental Mutagene

View full text Add to dashboard Cite

Investigation of the level of DNA double-strand breaks and mechanisms of cell death under irradiation of lung cancer and melanoma cells with ultra-high dose rate photon radiation

Кулинич

Yua

et al. 2018

BRSMU

View full text Add to dashboard Cite

Research into the effects of radiation delivered at ultrahigh dose rates > 1 × 107 Gy/min to biological objects is a new promising area of radiobiology. The unique characteristics of the high-current nanosecond electron accelerator Mir-M enable its use in medical and biological research, specifically in the experiments aimed at investigating the effect of therapeutic doses at a dose rate up to 100 MGy/s. In this work we study the effects of ultrahigh dose rate photon radiation on human lung carcinoma (A549) and melanoma (MelMtp-x) cells lines and compare them with those of the therapeutic gamma unit Rokus-AM. We show that ultrahigh dose rates induce more significant damage in the studied cell lines at doses between 2 and 7 Gy, radioresistant melanoma being more sensitive to photon radiation delivered at ultrahigh dose rates.

show abstract

Micronucleus frequencies and clonogenic cell survival in TK6 cells exposed to changing dose rates under controlled temperature conditions

Abstract: We speculate that the high level of MN is probably related to a delayed elimination of damaged cells by interphase death, as opposed to mechanisms relating to DNA damage and repair.

Cited by 4 publications

References 27 publications

Can high dose rates used in cancer radiotherapy change therapeutic effectiveness?

Can high dose rates used in cancer radiotherapy change therapeutic effectiveness?

Translocation dose-response curve for 137Cs γ-rays: Dose validation at various dose rate and changing dose rate conditions

Investigation of the level of DNA double-strand breaks and mechanisms of cell death under irradiation of lung cancer and melanoma cells with ultra-high dose rate photon radiation

Contact Info

Product

Resources

About