Micronucleus Assay for Evaluation of Genotoxicity in Potentially Malignant and Malignant Disorders

Devi, Parvathi; Thimmarasa, VB; Mehrotra, Vishal; Arora, Pallak

doi:10.5005/jp-journals-10011-1103

Cited by 11 publications

(13 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings indicate a genotoxic damage of genotoxic agents and their metabolic products to exfoliated buccal epithelial cells. We found similar results in studies done by Parvathi et al and Halder et al [14,15] In the past, there are studies reported with significant micronuclei values in PMDs as compared to normal participants. In our study, we also found similar results.…”

Section: Discussionsupporting

confidence: 92%

Evaluation of micronuclei in betel quid chewers, potentially malignant oral disorders, and oral squamous cell carcinoma patients: A cytological assay

Somashekhar¹,

Kamath²

2016

JCRI

View full text Add to dashboard Cite

Background:The micronuclei are round to oval extranuclear cytoplasmic bodies associated with chromosomal aberrations. Micronuclei are reported to be prognostic biomarkers in the screening of potentially malignant disorders (PMDs) and oral cancer. The present study was done to determine, compare, and correlate micronuclei count in normal participants, betel quid chewers, PMDs, and oral squamous cell carcinoma patients (OSCC). Materials and Methods:The buccal smears in each group were stained by Papanicolaou technique using commercially available staining kit RAPIDPAP®. Micronuclei were identified and counted in all the cases using standardized techniques. Comparative statistical evaluation was done within and among the groups. Results: The median value of micronuclei in each group was assessed using KruskalWallis test. Intergroup comparison was done using Dunn's Multiple Comparison Test. A significant increase in the median values of micronuclei in betel quid chewers was found compared to normal participants. The median values of micronuclei were significantly higher in OSCC patients followed by oral submucous fibrosis (OSF), leukoplakia, oral lichen planus, and betel quid chewers as compared to normal participants. Conclusion:The median values of micronuclei are indicative of cytogenetic damage induced by potential carcinogens. Micronuclei counts are reliable cytogenetic tools in the progression of carcinogenesis.

show abstract

Section: Discussionsupporting

confidence: 92%

Evaluation of micronuclei in betel quid chewers, potentially malignant oral disorders, and oral squamous cell carcinoma patients: A cytological assay

Somashekhar¹,

Kamath²

2016

JCRI

View full text Add to dashboard Cite

show abstract

“…These observations indicate an increase in cytogenetic damage of the epithelial cells. This is in accordance with the studies conducted by Sangle et al, Devi et al, and Halder et al [3,12,13] The mean difference between the numbers of Micronuclei in PMD group and OSCC group was statistically highly significant (P < 0.05). This is similar to the study conducted by Gupta et al (2011).…”

Section: Frequency Of Micronuclei In Oral Potentially Malignant Disorsupporting

confidence: 92%

Comparative Study of Frequency of Micronuclei Using Papanicolaou Stain in Potentially Malignant Disorders and Oral Squamous Cell Carcinoma

2018

IJDA

View full text Add to dashboard Cite

Background: Early recognition is crucial for successful treatment and good prognosis of oral cancer. A simple, noninvasive, and reasonably effective technique for initial evaluation of a suspicious oral lesion are exfoliative cytology. Micronuclei being structures presenting after genomic damage can be used as a screening tool in early detection of cancer. Method: A total of 60 subjects, 30 with potentially malignant disorders (PMD) (oral lichen planus and oral submucous fibrosis), 15 with oral squamous cell carcinoma (OSCC), and 15 with apparently normal mucosa, were included in the study. Cytological smears were collected from these groups and micronuclei were examined using Papanicolaou stain. One-way ANOVA was used to compare the mean micronuclei distribution among different study groups. Results: An increase in the frequency of micronuclei from normal to PMD to cancerous lesions was observed. Analysis has shown significant differences between various study groups (P < 0.05), with highest score observed in OSCC group, intermediary in PMD, and least in controls. Conclusion: The oral mucosal micronuclei can be used as a marker of epithelial carcinogenic progression.

show abstract

“…[14] It is evident that our findings are comparable to that of Kamboj and Mahajan (2007). [7,10,15,24,25] To summarize the results, assessment of the frequency of MN in oral exfoliated cells is an innovative technique, which holds promise for monitoring the genetic damage in PMDs.…”

Section: Discussionsupporting

confidence: 64%

“…[9] Micronuclei can be used as a diagnostic tool, to screen individuals having PMDs, and know whether they are at risk of transformation to malignancy. [10] Hence, the present study was performed with aim of evaluating MN frequencies in PMDs using Feulgen stain. Furthermore, frequencies of MN in different grades of dysplasia of these PMDs were evaluated.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Micronucleus Frequencies in Exfoliated Buccal Cells of Potentially Malignant Disorder Subjects Using Feulgen Stain

2019

IJDA

View full text Add to dashboard Cite

Introduction:Oral squamous cell carcinoma generally arises from oral potentially malignant disorders (OPMDs) through a complex process of genetic alterations such as chromosomal alterations, epigenetic alterations, and/or DNA changes. If these lesions are detected in early stages, i.e., during stage of PMDs through specific and sensitive tests, it can markedly improve the survival rates of patients. Micronucleus (MN) is one such suitable and reliable test to detect genotoxic effects of various carcinogens. Aims: The aim of the present study was to evaluate and compare the levels of micronuclei in OPMDs as well as to correlate micronuclei frequency in different grades of dysplasia. Materials and Methods: The study cases were divided into two groups, Group I consisting of thirty healthy controls and Group II of thirty histopathologically proven cases of OPMDs. The exfoliated cells were collected from buccal mucosa/lesional area of both study groups and were stained with Feulgen stain and evaluated under microscope for MN. Statistical analysis was done using ANOVA test and correlation was calculated using Pearson's correlation using SPSS 18.0 software. Results: According to the present study, MN and MN percentage (MN%) increased from normal to potentially malignant disorder cases with statistically significant difference. A highly positive correlation (0.946) between grades of dysplasia and MN formation was obtained. Conclusion: The present study concludes that the occurrence of MN and MN% increases from controls to potentially malignant disorder cases. Among PMDs, the occurrence of MN and MN% was highest in patients of oral submucous fibrosis followed by leukoplakia and lichen planus patients. And finally, with increase in grades of dysplasia, in PMD subjects, the formation of MN increases.

show abstract

Micronucleus Assay for Evaluation of Genotoxicity in Potentially Malignant and Malignant Disorders

Cited by 11 publications

References 9 publications

Evaluation of micronuclei in betel quid chewers, potentially malignant oral disorders, and oral squamous cell carcinoma patients: A cytological assay

Evaluation of micronuclei in betel quid chewers, potentially malignant oral disorders, and oral squamous cell carcinoma patients: A cytological assay

Comparative Study of Frequency of Micronuclei Using Papanicolaou Stain in Potentially Malignant Disorders and Oral Squamous Cell Carcinoma

Evaluation of Micronucleus Frequencies in Exfoliated Buccal Cells of Potentially Malignant Disorder Subjects Using Feulgen Stain

Contact Info

Product

Resources

About