Micronucleus Assay: An Early Diagnostic Tool to Assess Genotoxic Changes in Patients with Tobacco Use, Oral Leukoplakia and Oral Submucous Fibrosis

Kohli, Moulshree; Ahuja, Puneet; Mehendiratta, Monica; Sharma, Mohit; Dutta, Jahnobi

doi:10.7860/jcdr/2017/27711.10567

Cited by 7 publications

(11 citation statements)

References 26 publications

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“…The frequency of smokeless tobacco use a day was variable and measured in different units of measurement, but most of the studies did not report this information (6,8,11-13,16,18,21,22,24,26).…”

Section: Resultsmentioning

confidence: 99%

“…In the studies that report the gender of participants, most of them were male (1,6,7,13,15-17,23,28,29,32), with exception of three studies (21,27,31). The range of participants age varied from 18 to 80 (1,6-8,11,13,16-18,22-24,27).…”

Section: Resultsmentioning

confidence: 99%

“…According to the local of smear collection of exfoliated cells, most of studies (n = 19) used the cheek mucosa (1,6,8,11,12,15,18,21-25,27). The number of cells counting per participant ranged from 20 to 3500, with the great majority (14 out from 25 studies) counting on 1000 cells (6,8,11,15,17,21,22,26-28,30). In relation to the magnification used to count the cells, it varied from 40 x (22,30,34) to 1000 x (1,24).…”

Section: Resultsmentioning

confidence: 99%

“…The number of cells counting per participant ranged from 20 to 3500, with the great majority (14 out from 25 studies) counting on 1000 cells (6,8,11,15,17,21,22,26-28,30). In relation to the magnification used to count the cells, it varied from 40 x (22,30,34) to 1000 x (1,24).…”

Section: Resultsmentioning

confidence: 99%

“…Few full-text studies reported adequately items to allow evaluation of the selection bias, confounders, blinding, data collection methods, withdrawals and dropouts. In summary, from the 25 studies, seven were considered strong (7,8,13,23,31,33,34), one was considered weak (26) and the majority of them (sixteen studies) were considered to have a moderate quality (1,6,11,12,15-18,21,22,24,27).…”

Section: Resultsmentioning

confidence: 99%

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The smokeless tobacco habit and DNA damage: A systematic review and meta-analysis

Geus¹,

Wambier²,

Loguércio³

et al. 2019

Med Oral

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Background The aim of this systematic review was to evaluate the frequency of micronuclei or other DNA damage in the oral mucosa of adults that have smokeless tobacco habits compared to adults that not have these habits. Material and Methods We searched PubMed, Scopus, Web of Science, LILACS, BBO and Cochrane Library and SIGLE. We also surveyed gray literature. We included only clinical trials that compare the frequency of micronuclei or other DNA damage in the oral mucosa of adults that have smokeless tobacco habits compared to adults that not have these habits. Quality assessments of the selected trials were evaluated by two independent reviewers, using the Effective Public Health Practice Project – (EPHPP) with modifications. Results After the database screening and removal of duplicates, 2574 studies were identified. After title screening, 172 studies remained, and this number was reduced to 25 after careful examination of the abstracts. The standardized mean difference of the frequency of micronuclei between groups was 1.88, with a 95% confidence interval of 1.40 to 2.36 ( p < 0.00001). In all analyses heterogeneity was detected. Conclusions Despite the heterogeneity of studies, the frequency of micronuclei was significant bigger in adults who have the smokeless tobacco habit when compared to those not have this habit. The same occurred with the frequency of binucleated cells, karyolisis and karyorrhexis. Key words: Micronucleus tests, Tobacco, smokeless, DNA damage.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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The smokeless tobacco habit and DNA damage: A systematic review and meta-analysis

Geus¹,

Wambier²,

Loguércio³

et al. 2019

Med Oral

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Micronuclei and What They Can Tell Us in Cytogenetic Diagnostics

2018

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Effect of Vitamin D Serum Levels and Supplementation on Atopic Dermatitis: A Systematic Review and Meta-analysis

Ng¹,

Yew

2022

Am J Clin Dermatol

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Background Previous studies have shown that serum 25-hydroxyvitamin D (25(OH)D) may be associated with atopic dermatitis (AD), and that vitamin D (VD) supplementation may decrease AD severity. Objective The aim of this study was to investigate the association between serum 25(OH)D level and AD, and the effect of VD supplementation on AD severity, while providing stratified analyses based on latitude and region. Methods A systematic review was performed on all published studies in MEDLINE, Embase, and Cochrane Library databases that analyzed effects of serum 25(OH)D and VD supplementation on AD. Results This systematic review and meta-analysis includes 20 studies with 1882 cases of AD. We found significantly lower 25(OH)D levels in AD patients compared with healthy controls (HC) (p < 0.001), significantly lower 25(OH)D levels in severe AD compared with both mild and moderate AD (p < 0.001), and VD supplementation improved AD symptoms (p < 0.001). Limitations Factors like seasonal and environmental changes, sunlight exposure, and cultural practices may confound the relationship between serum 25(OH)D and AD severity. There are limited randomized controlled trials that assess this association. Conclusion Overall, lower serum 25(OH)D is associated with more severe AD, and VD supplementation may help lower AD severity. Further research is needed to confirm the presence and direction of causality of the relationship between VD and AD pathogenesis. Key PointsAtopic dermatitis (AD) patients had significantly lower 25-hydroxyvitamin D (25(OH)D) levels compared with healthy controls. Patients with severe disease had lower 25(OH)D levels than patients with mild and moderate disease. AD symptoms improved after vitamin D (VD) supplementation.Further research on efficacy and optimal dosage of VD supplementation in AD treatment is required.

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Micronucleus Assay: An Early Diagnostic Tool to Assess Genotoxic Changes in Patients with Tobacco Use, Oral Leukoplakia and Oral Submucous Fibrosis

Cited by 7 publications

References 26 publications

The smokeless tobacco habit and DNA damage: A systematic review and meta-analysis

The smokeless tobacco habit and DNA damage: A systematic review and meta-analysis

Micronuclei and What They Can Tell Us in Cytogenetic Diagnostics

Effect of Vitamin D Serum Levels and Supplementation on Atopic Dermatitis: A Systematic Review and Meta-analysis

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