Micronuclei in breast aspirates. Is scoring them helpful?

Hemalatha, A; Suresh, T N; Harendrakumar, M L

doi:10.4103/0973-1482.136588

Cited by 12 publications

(11 citation statements)

References 16 publications

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“…1.8 ± 1.9 in the benign group. [ 7 8 ] MN score of ≤1 had a high sensitivity (100%) and specificity (99%) for confirming benign cases in our study.…”

Section: Discussionsupporting

confidence: 52%

“…Few studies of micronucleus on breast cytology have compared the benign and malignant groups. [ 7 8 9 ] No studies have been conducted comparing micronucleus frequency in benign, adenosis, usual/atypical ductal hyperplasia, and malignant cases according to the grade in a single study group.…”

Section: Discussionmentioning

confidence: 99%

“…[ 4 5 6 ] Few studies have been done in breast cytology with controversial results. [ 7 8 9 ] This study was conducted to find any difference in micronucleus scores in breast cytology aspirates along the benign, adenosis, usual/atypical ductal hyperplasias, and malignant categories and its usefulness in classification of borderline cases which are difficult to diagnose on cytology smears.…”

Section: Introductionmentioning

confidence: 99%

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Micronucleus study on breast cytology aspirate smears and its diagnostic utility

Sylvia

Baskaran

Bhat

2018

J Cytol

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Background:Micronucleus is a small fragment of nucleus present in the cells which have undergone chromosomal damage. It is used as a biomarker of genomic damage.Aims:We aim to study the presence of micronucleus on breast cytology smears and the use of scoring the micronucleus as an additional criteria for the classification of breast lesions with emphasis on borderline gray zone categories.Settings and Design:This is a cross-sectional retrospective descriptive study.Materials and Methods:Retrospective analysis of breast cytology smears received over a period of 2 years formed the basis of the study. Micronucleus scoring was done by counting the number of micronuclei in 1000 epithelial cells under oil immersion and compared in the benign, adenosis, usual/atypical ductal hyperplasia, and the three grades of infiltrating ductal carcinomas.Statistical Analysis:Descriptive analyses and one-way analysis of variance was used for statistical analysis.Results:Of the 243 cases, the average statistically significant (P < 0.05) micronuclei scores of the benign (190), adenosis (7), usual (11)/atypical (5) hyperplasia, grade 1, 2, and 3 carcinomas (30) were 0.5, 2, 2.9, 6.6, 13.2, 20.6, and 27.5, respectively (the corresponding median scores were 0.5, 2, 3, 6, and 20, respectively). Micronucleus score of ≤1 had a high sensitivity (100%) and specificity (99%) in confirmation of benign cases. Micronucleus score of ≥5 and <10 had a moderate sensitivity (60%) but a high specificity of 99% in detecting atypical ductal hyperplasia. Micronucleus score of ≥10 had a high sensitivity (96%) and specificity (99%) of detecting carcinomas.Conclusion:Micronucleus scores showed a gradual increase across the categories proving the gradual occurrence of genomic damage. Micronucleus scoring serves as an additional criterion for the diagnosis of breast lesions.

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“…1.8 ± 1.9 in the benign group. [ 7 8 ] MN score of ≤1 had a high sensitivity (100%) and specificity (99%) for confirming benign cases in our study.…”

Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Micronucleus study on breast cytology aspirate smears and its diagnostic utility

Sylvia

Baskaran

Bhat

2018

J Cytol

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“…In the present study, we evaluated the presence and frequency of MN in pleural fluid cytology. The limited studies in this field clearly pointed out that neoplastic lesions had high MN counts compared to their benign counterparts (22)(23)(24). Consistently with the literature, we found that all the cytological smears with a malignant outcome had a significantly higher MN count compared to benign ones (P < 0.001, Table 1).…”

Section: Discussionsupporting

confidence: 90%

Increased micronucleus count predicts malignant behavior in pleural e usion uid

2018

Turk J Med Sci

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Background/aim: Micronucleus (MN) frequency is used as a biomarker of chromosomal damage, genome instability, and cancer risk. The aim of this study was to evaluate the diagnostic usefulness of MN frequency to differentiate between malignant and benign pleural effusion samples.Materials and methods: Retrospectively, 78 pleural fluid cytology samples (including 20 cases of benign reactive mesothelial cells, 22 cases of suspicious cytology, and 36 cases of malignant cytology) were examined. The number of micronucleated cells in 1000 wellpreserved cells was counted. Statistical tests were performed to compare the study groups. Recover operating characteristics (ROC) curve analysis was performed to suggest a cut-off value for predicting malignant behavior. Results:We evaluated a total of 78 cases of pleural effusion cytology. The number of micronucleated cells was significantly higher in cases with malignant outcome compared to cases with benign outcome. We observed that malignant samples had more micronucleated cells than suspicious ones, and suspicious cases had more micronucleated cells than reactive ones. There was a significant difference among all study groups. In addition, the frequency of MN-containing cells in suspicious cases correlates well with their outcomes. Conclusion:The results of this study reveal that there is an absolute, consistent, and proportional relationship between MN counts and malignancy in cytological samples of pleural effusions. MN scoring may be a helpful diagnostic tool for distinguishing malignant effusions from benign ones, and may be used as an adjunct tool to predict malignant behavior in challenging cases.

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“…The usefulness of micronuclei in distinguishing malignant lesions from benign lesions using cytological specimens has been well clariied [41][42][43]. A micronucleus is the small nucleus that forms whenever a chromosome or its fragment is not incorporated into one of the daughter nuclei during cell division and it serves as an indicator of CIN.…”

Section: Morphological Markers Of Chromosomal Instabilitymentioning

confidence: 99%