Micronuclei and other nuclear abnormalities on Caiman latirostris (Broad-snouted caiman) hatchlings after embryonic exposure to different pesticide formulations

González, Evelyn Cecilia López; Larriera, Alejandro; Siroski, Pablo Ariel; Poletta, Gisela Laura

doi:10.1016/j.ecoenv.2016.10.035

Cited by 33 publications

(15 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have demonstrated the usefulness of assessing nuclear abnormalities as a complementary tool together with micronuclei frequency to diagnose the exposure to genotoxic agents (Braham et al 2017; López González et al 2017). However, ENA frequency seems to be a more sensitive marker of genotoxic damage than micronuclei alone (Santos et al 2017b).…”

Section: Discussionmentioning

confidence: 99%

Mercury Uptake Affects the Development of Larus fuscus Chicks

Santos

Sotillo

Gupta

et al. 2020

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Current emission and mobilization rates of mercury (Hg) in the environment pose extensive threats to both wildlife and human health. Assessing the exposure risk and effects of Hg contamination in model species such as seabirds is essential to understand Hg risks at the population and ecosystem levels. The lesser black-backed gull (Larus fuscus), a generalist seabird species, is an excellent model species because it forages in both marine and terrestrial habitats, which in turn differ in their Hg exposure risk. To identify possible deleterious effects of Hg exposure on developing L. fuscus chicks, a dietary experiment was carried out and chicks were provided a marine, terrestrial, or mixed diet. The effects of embryonic and dietary Hg exposure on chick body condition and physiological state were assessed at different developmental stages until fledging age (30 d). Overall physiological condition was lower in chicks fed a predominantly marine diet, which coincided with higher Hg loads in blood and primary feathers. However, no effect of dietary uptake of Hg was observed on body condition or in terms of genotoxic damage. Body condition and genotoxic damage correlated instead with Hg exposure during embryonic development, which seems to indicate that embryonic exposure to Hg may result in carry-over effects on later chick development.

show abstract

Section: Discussionmentioning

confidence: 99%

Mercury Uptake Affects the Development of Larus fuscus Chicks

Santos

Sotillo

Gupta

et al. 2020

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…The results described here provide first evidence of hepatic toxic effect of pure Gly on a terrestrial reptile. Indeed, previous investigations on reptiles focused only on GBH effects, mainly on marine and freshwater turtles [11, 35], embryos [12, 36], or adult immune system [13, 14]. Our data demonstrate that pure Gly administered orally is toxic and exerts a xenoestrogenic action in the liver of Podarcis siculus even at doses that can be considered low if compared to the Gly working solution generally used.…”

Section: Discussionmentioning

confidence: 72%

How Glyphosate Impairs Liver Condition in the Field Lizard Podarcis siculus (Rafinesque-Schmaltz, 1810): Histological and Molecular Evidence

Verderame

Scudiero

2019

BioMed Research International

View full text Add to dashboard Cite

The potential toxicity of glyphosate, a widely used broad-spectrum herbicide, is currently a great matter of debate. As vertebrate insectivores, lizards protect plants from herbivorous insects increasing plant biomass via the trophic cascade and represent an important link between invertebrates and higher predators. A negative effect of glyphosate on lizards’ survival could have major impacts at the ecological levels. In this study, we investigated the effects of the exposure to low doses of glyphosate on the liver of the wall lizard Podarcis siculus, a suitable bioindicator of soil pollution. Two different doses of pure glyphosate (0.05 and 0.5 μg/kg body weight) were orally administered every other day for 3 weeks to sexually mature males and females. The results demonstrated that both doses, despite being very low, are toxic for the liver that showed clear signs of suffering, regardless of sex. The histological analysis provided a scenario of severe hepatic condition, which degenerated until the appearance of fibrotic formations. The morphological observations were consistent with a loss of liver physiological functions. Immunocytochemical investigations allowed us to detect an involvement of antioxidant/cytoprotective proteins, such as superoxide dismutase 1 (Cu/Zn SOD, known as SOD1), glutathione peroxidase 1 (GPx1), metallothionein (MT), and tumor suppressor protein 53, (p53) suggesting that the liver was trying to react against stress signals and damage induced by glyphosate. Finally, in situ hybridization and Real-Time PCR analysis showed the upregulation of estrogen receptor α and vitellogenin gene expression, thus demonstrating the xenoestrogenic action of glyphosate. The imbalance of the hormonal homeostasis could threaten the lizards’ reproductive fitness and survival, altering the trophic cascade.

show abstract

“…Most of the biomarkers used to date to identify contamination stress are based on alterations in biochemical parameters and/or cytological or histological abnormalities that can be correlated with the presence of toxins in the environment [9,10]. Specifically, studies made to evaluate the impact of pesticides in C. latirostris include biomarkers of DNA damage, oxidative damage to DNA and lipids, antioxidant enzymes such as Catalase (CAT) and Superoxide dismutase (SOD) and immunological alterations [8,11,12,13,14,15,16,17,18]. These toxicological endpoints are useful and convenient tools for monitoring environmental quality, assessing chemical risks and safety, investigating threshold values, and evaluating the vulnerability of organisms to specific chemicals or toxins, such as pesticides [19].…”

Section: Introductionmentioning

confidence: 99%

Identification and evaluation of antioxidant and reference genes for quantitative real-time PCR in blood of Caiman latirostris

Odetti

Paravani

Simoniello

et al. 2021

Heliyon

View full text Add to dashboard Cite

The quantitative real-time polymerase chain reaction (qPCR) has been one of the most promising approaches to perform rapid and accurate quantification of DNA in various biological systems. The aim of this study was to standardized the qPCR technique for the analysis of important genes involved in the main routes of antioxidant defense against reactive oxygen species (catalase: cat and superoxide dismutase: sod) and evaluate the stability of different reference genes in blood of Caiman latirostris hatchlings. The stability of the reference genes, β-actin, glyceraldehyde 3-phosphate dehydrogenase (gapdh) and ribosomal protein L8 (rpl8) was determined using the comparative ΔCt, NormFinder, geNorm, BestKeeper and RefFinder. Then, cat and sod genes were normalized with each reference gene and their mRNA abundances were determined through the qPCR. Stability of genes was ranked through the different methods in the following order: β-actin, rpl8 and gapdh, under normal physiological conditions. The results reveal that cat and sod genes present a similar relative mRNA abundance with β-actin and rpl8. This is the first report of the analysis of antioxidant mRNA as potential biomarkers of oxidative stress in blood for all crocodilians species. Besides, we determined the stability of different reference genes that can be used for normalization of mRNA abundance patterns in blood of C. latirostris, without the need to sacrifice the animals.

show abstract

Micronuclei and other nuclear abnormalities on Caiman latirostris (Broad-snouted caiman) hatchlings after embryonic exposure to different pesticide formulations

Cited by 33 publications

References 56 publications

Mercury Uptake Affects the Development of Larus fuscus Chicks

Mercury Uptake Affects the Development of Larus fuscus Chicks

How Glyphosate Impairs Liver Condition in the Field Lizard Podarcis siculus (Rafinesque-Schmaltz, 1810): Histological and Molecular Evidence

Identification and evaluation of antioxidant and reference genes for quantitative real-time PCR in blood of Caiman latirostris

Contact Info

Product

Resources

About