Micronucleated erythrocytes in splenectomized patients with and without chemotherapy

G, Zúñiga; Torres-Bugarı́n, Olivia; Ramı́rez-Muñoz, M.P; Delgado-Lamas, José Luis; Loza-Saldaña, R. De; Cantú, J. M.

doi:10.1016/s0165-1161(96)90244-7

Cited by 26 publications

(6 citation statements)

References 13 publications

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“…Indeed, due to the efficiency of the spleen at removing circulating MNE, not even exposure to micronucleogenic drugs increases the frequency of MNE in humans (6-7,12). As a result, MNE can typically only be observed in humans who have spleen dysfunction (1,6,13-14) or have been splenectomized (15)(16). In contrast, the immature reticuloendothelial system of Preterm Newborns (PN) does not efficiently remove MNE from circulation and it is possible to observe MNE in the peripheral blood of PN (9).…”

Section: Introductionmentioning

confidence: 99%

Eritrocitos micronucleados en recién nacidos pretérmino en relación con la patología materna

Batista-González¹,

Corona-Rivera²,

Gómez‐Meda³

et al. 2006

Rev Biomed

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Introducción. La prueba de micronúcleos detecta daño al ADN. Algunas patologías incrementan la frecuencia de eritrocitos micronucleados (EMN) debido a la producción de radicales libres asociados con el proceso de la enfermedad. En el presente trabajo, investigamos si la frecuencia de EMN en el embrión se incrementa por patologías que ocurren durante el embarazo y que involucran la formación de radicales libres. Material y métodos. Mediante la prueba de micronúcleos (MN) in vivo en eritrocitos de sangre periférica, las frecuencias de EMN en recién nacidos pretérmino (RNP) de madres sin patología fueron comparadas con RNP de madres con diabetes mellitus (DM), hipertensión arterial sistémica (HAS) o infección vaginal (IV). Las muestras se agruparon respecto al género, patología materna e ingesta materna de ácido fólico. Resultados. Las frecuencias de EMN y eritrocitos policromáticos micronucleados (EPCMN) en RNP de madres con patología fueron mayores que los de madres sin patología (P‹0.02). RNP de madres con DM tuvieron frecuencia elevada de EMN y EPCMN (P‹0.01), mientras que los EMN incrementaron (P‹0.03) en los RNP de madres con IV. Los EMN en RNP de madres con HAS se incrementaron y se observó disminución en niñas RNP y en RNP cuyas madres recibieron ácido fólico (P›0.05). Los resultados fueron evaluados por medio de la prueba U-Mann Whitney. Discusión. Nuestros resultados muestran aumento en los EMN de RNP de madres con patologías que involucran incremento en la producción de radicales libres.

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Section: Introductionmentioning

confidence: 99%

Eritrocitos micronucleados en recién nacidos pretérmino en relación con la patología materna

Batista-González¹,

Corona-Rivera²,

Gómez‐Meda³

et al. 2006

Rev Biomed

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“…An MN can be found in any tissue, whether it be plant,12 laboratory animal,9 wild animal14 – 16 and even in human tissue 11. The following tissues are useful for the biomonitoring of human populations: lymphocytes,11 reticulocytes9 and erythrocytes 16 – 18. Exfoliated epithelial cells are also biomarkers of genotoxicity, with the advantage that they do not need to be kept in vivo 19 – 21.…”

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confidence: 99%

Anabolic androgenic steroids induce micronuclei in buccal mucosa cells of bodybuilders

et al. 2007

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Objective:To evaluate genotoxicity of anabolic androgenic steroids (AAS) in male bodybuilders by a micronucleus assay in buccal mucosa cells.Methods:11 male bodybuilders volunteered to participate in this study and two groups were formed: group 1 (n = 6), without AAS consumption and group 2 (n = 5), with AAS consumption. A sample of buccal epithelium was taken from each participant once a week for 6 weeks. Samples were fixed, stained and analysed by a light microscope, and 2000 cells were counted from each slide. Results are expressed as micronucleated cells (MNC) per 1000 cells and were analysed by the Mann–Whitney U test and Wilcoxon’s test.Results:A marked increased in MNC was seen in bodybuilders with AAS consumption compared with those without AAS consumption (mean (SD) 4.1 (2.4) MNC/1000 cells vs 0.4 (0.4) MNC/1000 cells, respectively; p<0.004). Intragroup comparisons showed no differences in the MNC frequencies during the sampling time in group 1, whereas the MNC frequency in group 2 varied significantly, reaching the highest MNC frequencies in the third and fourth week of sampling (5.9 (2.4) MNC/1000 cells; 5.8 (1.8) MNC/1000 cells, respectively); frequency in the first sampled week was 1.1 (0.1) MNC/1000 cells. Significant differences in all sampled weeks were found between the two groups.Conclusion:AAS consumption increased the frequency of MNC from buccal mucosa in bodybuilders.

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“…A possible cytotoxic effect was observed with the single 0.387-mg/kg dose of colchicine, as the increases in PMNC following this dose were not significant. Cytotoxicity may have affected cellular division and decreased the number of MNC [Zúñiga et al, 1996]. Single doses of 50 and 80 mg/kg cyclophosphamide produced significant increases in PMNC frequency in the first and second proestrus (P Ͻ 0.05), whereas a single dose of 20 mg/kg cyclophosphamide resulted in a significant increase only in the first proestrus (P Ͻ 0.05).…”

Section: Resultsmentioning

confidence: 99%

Induction of micronuclei in proestrus vaginal cells from colchicine‐ and cyclophosphamide‐treated rats

Zúñiga‐González

Gómez‐Meda

Zamora‐Pérez

et al. 2003

Environ and Mol Mutagen

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Micronucleated erythrocytes in splenectomized patients with and without chemotherapy

Cited by 26 publications

References 13 publications

Eritrocitos micronucleados en recién nacidos pretérmino en relación con la patología materna

Eritrocitos micronucleados en recién nacidos pretérmino en relación con la patología materna

Anabolic androgenic steroids induce micronuclei in buccal mucosa cells of bodybuilders

Induction of micronuclei in proestrus vaginal cells from colchicine‐ and cyclophosphamide‐treated rats

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