Micronized Gel Electrophoresis: Construction and Its Versatile Applications

Biyani, Manish; Nishizawa, Hiroshi; Miyatani, Yoshihide; Kanaumi, Eiichi; Eli, Pharhad; Kinoshita, Yasunori; Nishigaki, Koichi

doi:10.1246/cl.2001.138

Cited by 1 publication

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“…Temperature gradient gel electrophoresis (TGGE) (Thatcher and Hodson, 1981) enables us to measure the DNA sequence-inherent melting temperature , for which the device can be miniaturized to a size of square inches without losing accuracy (Biyani et al, 2001;Tominaga, 2006). In this study, a µTGGE apparatus, μ-TG (TAITEC, Japan) was used with a specifically fabricated gel cassette.…”

Section: µTgge (Temperature Gradient Gel Electrophoresis) Analysismentioning

confidence: 99%

Genome profiling (GP) as an effective tool for monitoring culture collections: A case study with Trichosporon

Hamano

Ueno-Tsuji

Tanaka

et al. 2012

Journal of Microbiological Methods

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Species identification and classification of a large number of microbes are essential and heavy workloads in culture collections and relevant laboratories. The identification of species usually requires different methods depending on species. Therefore, the development of a method which is simple and applicable to any organisms will lessen the burdens, increase the reliability of databases and thus enhance the science on microbes. The genome profiling (GP) method, developed previously, was found effective in monitoring authenticities of all strains/species tested in culture collections and expectedly various species, which was shown by applying the GP and the conventional sequencing methods to identifying and classifying species/strains belonging to the genus Trichosporon (38 strains; 16 species). Small differences between strains (11 strains of Trichosporon asahii and 4 strains of Trichosporon coremiiforme) can be reliably discriminated by GP, which was unsuccessful in the conventional sequencing approach. Importantly, seven possible false-assignments contained in the database were all pointed out by the GP method with near-perfect correctness, showing the power of the GP method.GP was shown to be a potent tool for rapidly and correctly monitoring species and strains of fungi in culture collections owing to its simple and informative natures.

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Section: µTgge (Temperature Gradient Gel Electrophoresis) Analysismentioning

confidence: 99%