Microneedles coated with peanut allergen enable desensitization of peanut sensitized mice

Shakya, Akhilesh Kumar; Ingrole, Rohan S.J.; Joshi, Gaurav; Uddin, Jasim; Anvari, Sara; Davis, Carla M.; Gill, Harvinder Singh

doi:10.1016/j.jconrel.2019.09.022

Cited by 44 publications

(31 citation statements)

References 68 publications

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“…The microneedle drawn in 2D is bent by 90 degrees to create a 3D microneedle. Needle tips or rough surfaces can be cleaned using electropolishing (Gill et al 2007a ; Gill et al 2007b ; Shakya et al 2019 ).…”

Section: Microneedle Fabrication Techniquesmentioning

confidence: 99%

“…Generally, it is economical to place the drugs only at the tip where the microneedle enters the actual skin. In the case of dip coating, the drug-coated area can be controlled via regulating the depth to which the microneedle is dipped into the coating formulation (Gill et al 2007a ; Gill et al 2007b ; Shakya et al 2019 ). The drug-coated area can be determined by controlling the surface tension of the coating formulation, thus regulating the spreading of the microneedle.…”

Section: Introductionmentioning

confidence: 99%

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Microneedle for transdermal drug delivery: current trends and fabrication

Jung

Jin

2021

J. Pharm. Investig.

189

127

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Background Transdermal delivery has the advantage of bypassing the first-pass effect and allowing sustained release of the drug. However, the drug delivery is limited owing to the barrier created by the stratum corneum. Microneedles are a transdermal drug delivery system that is painless, less invasive, and easy to self-administer, with a high drug bioavailability. Area covered The dose, delivery rate, and efficacy of the drugs can be controlled by the microneedle design and drug formulations. This review introduces the types of microneedles and their design, materials used for fabrication, and manufacturing methods. Additionally, recent biological applications and clinical trials are introduced. Expert opinion With advancements made in formulation technologies, the drug-loading capability of microneedles can be improved. 3D printing and digital technology contribute to the improvement of microneedle fabrication technology. However, regulations regarding the manufacture of microneedle products should be established as soon as possible to promote commercialization.

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Section: Microneedle Fabrication Techniquesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microneedle for transdermal drug delivery: current trends and fabrication

Jung

Jin

2021

J. Pharm. Investig.

189

127

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“…The bio-acceptability and dissolution of the polymer inside the skin make it possible for releasing the allergens at a desirable pace. Among these microneedle arrays (MNAs), coated and dissolving MNAs have been investigated to deliver allergens or influenza vaccines through the skin to activate immune system (61)(62)(63)(64)(65). For instance, Spina et al used microneedle arrays superficially coated with birch pollen on each microneedle to deliver the allergens into the skin in humans demonstrating an improved desensitization efficacy compared with tape-stripping or skin prick testing (39).…”

Section: Microneedle Arrays (Mna)mentioning

confidence: 99%

“…For instance, Spina et al used microneedle arrays superficially coated with birch pollen on each microneedle to deliver the allergens into the skin in humans demonstrating an improved desensitization efficacy compared with tape-stripping or skin prick testing (39). Microneedles coated with peanut protein extract were fabricated to treat peanut allergy in murine models as well (61).…”

Section: Microneedle Arrays (Mna)mentioning

confidence: 99%

Innovative Systems to Deliver Allergen Powder for Epicutaneous Immunotherapy

Wang

Kong

2021

Front. Immunol.

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Allergy is a disorder owing to hyperimmune responses to a particular kind of substance like food and the disease remains a serious healthcare burden worldwide. This unpleasant and sometimes fatal allergic disease has been tackled vigorously by allergen-specific immunotherapy over a century, but the progress made so far is far from satisfactory for some allergies. Herein, we introduce innovative, allergen powder-based epicutaneous immunotherapies (EPIT), which could potentially serve to generate a new stream of technological possibilities that embrace the features of super safety and efficacious immunotherapy by manipulating the plasticity of the skin immune system via sufficient delivery of not only allergens but also tolerogenic adjuvants. We attempt to lay a framework to help understand immune physiology of the skin, epicutaneous delivery of powdered allergy, and potentials for tolerogenic adjuvants. Preclinical and clinical data are reviewed showing that deposition of allergen powder into an array of micropores in the epidermis can confer significant advantages over intradermal or subcutaneous injection of aqueous allergens or other epicutaneous delivery systems to induce immunological responses toward tolerance at little risk of anaphylaxis. Finally, the safety, cost-effectiveness, and acceptability of these novel EPITs are discussed, which offers the perspective of future immunotherapies with all desirable features.

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“…20 The application of CMNs in the treatment of peanut allergies has been recently studied. 21 The researchers found that a CMN group treated with peanut protein extract had signicant improvement in clinical symptoms and an upregulation of Th1 cytokines compared with an untreated group, suggesting the use of a CMN strategy successfully accelerated immune regulation involving the Th1 pathway. In the treatment of ocular disease, CMNs also play an important role; a more detailed introduction can be found elsewhere.…”

Section: Types Of Microneedlesmentioning

confidence: 99%