Microneedle-Based Delivery of Amphotericin B for Treatment of Cutaneous Leishmaniasis

Nguyen, Alexander K.; Yang, Kai‐Hung; Bryant, Kelsey E.; Joice, April C.; Werbovetz, Karl A.; Narayan, Roger J.

doi:10.1007/s10544-018-0355-8

Cited by 23 publications

(14 citation statements)

References 43 publications

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“…Chitosan has been investigated as a nanocarrier for oral administration [18], as well as an ingredient in an emulsion of nanoparticles [19]. Dermal delivery has been achieved with microneedles that generate micropores in the epidermis for the administration of an AmB solution [20]. For the topical treatment of CL, a combination of cyclodextrin plus AmB has been studied in a gel formulation [21], and ultradeformable liposomes have also been postulated [22].…”

Section: Introductionmentioning

confidence: 99%

Topical Amphotericin B Semisolid Dosage Form for Cutaneous Leishmaniasis: Physicochemical Characterization, Ex Vivo Skin Permeation and Biological Activity

et al. 2020

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Amphotericin B (AmB) is a potent antifungal successfully used intravenously to treat visceral leishmaniasis but depending on the Leishmania infecting species, it is not always recommended against cutaneous leishmaniasis (CL). To address the need for alternative topical treatments of CL, the aim of this study was to elaborate and characterize an AmB gel. The physicochemical properties, stability, rheology and in vivo tolerance were assayed. Release and permeation studies were performed on nylon membranes and human skin, respectively. Toxicity was evaluated in macrophage and keratinocyte cell lines, and the activity against promastigotes and intracellular amastigotes of Leishmania infantum was studied. The AmB gel remained stable for a period of two months, with optimal properties for topical use and no apparent toxic effect on the cell lines. High amounts of AmB were found in damaged and non-damaged skin (1230.10 ± 331.52 and 2484.57 ± 439.12 µg/g/cm2, respectively) and they were above the IC50 of AmB for amastigotes. Although there were no differences in the in vitro anti-leishmanial activity between the AmB solution and gel, the formulation resulted in a higher amount of AmB being retained in the skin, and is therefore a candidate for further studies of in vivo efficacy.

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Section: Introductionmentioning

confidence: 99%

Topical Amphotericin B Semisolid Dosage Form for Cutaneous Leishmaniasis: Physicochemical Characterization, Ex Vivo Skin Permeation and Biological Activity

et al. 2020

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“…Two-photon polymerization is a 3D lithography additive manufacturing technology. This process has been largely implemented for the production of conventional microneedles [95][96][97][98][99][100][101][102][103][104][105][106][107] and BMNs. 13,108,109 This method is flexible, highly precise and time-effective.…”

Section: Two-photon Polymerizationmentioning

confidence: 99%

Bioinspired microneedle patches: Biomimetic designs, fabrication, and biomedical applications

Makvandi

Maleki

Shabani

et al. 2022

Matter

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Nature contains abundant systems that can significantly alter their structures and properties to adapt to the surrounding environment. Through natural selection and unceasing evolution, hierarchical architectures and sophisticated strategies have been created by nature to achieve optimally adapted materials for biomedical applications. The development of microneedles has advanced to the next generation of bioinspired microneedles, with the goal of improving functions such as amelioration of mechanical properties and tissue adhesion. The biomimetic designs and structures of microneedles are highlighted in the present review. This is followed by an in-depth discussion of the fabrication approaches from molding techniques to 3D and 4D printing. The medical applications of bioinspired microneedles, including drug delivery, regenerative medicine, biopsy sampling and biosensing, are also discussed. Lastly, future opportunities and challenges with respect to clinical translation are also deliberated.

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“…Among the tools available for the fabrication of microstructures, TPP exhibits a series of unique and useful characteristics. [5][6][7][8][9][10][11] Among 3D printing processes, TPP is the only technique that can achieve "true" 3D writing, that is, the realization of complex objects without the need for a layer-by-layer approach. Furthermore, it can perform laser direct laser writing with the highest precision enabling feature sizes as small as 100 nm in a relatively straightforward manner.…”

Section: Throughput Of 3d Fabrication By Two-photon Polymerizationmentioning

confidence: 99%

“…6 These solid microneedles, with nominal heights of 500 μm, 750 μm, and 1000 μm (see Figure 5), were investigated for transdermal treatment of a parasitic condition that affects people in the developing work called cutaneous leishmaniasis. 7 The microneedles were used to treat female BALB/c mice infected with either the parasite Leishmania mexicana, which causes New World cutaneous leishmaniasis, or the parasite Leishmania major, which causes Old World cutaneous leishmaniasis. A topical dimethyl sulfoxide solution of the anti-leishmanial drug amphotericin B was applied to leishmaniasis lesions at a dose of 25 mg/kg for 10 consecutive days, either with or without preapplication of the microneedle arrays.…”

Section: Replication Of Microstructures By Moldingmentioning

confidence: 99%

Translation of laser-based three-dimensional printing technologies

et al. 2021

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Laser-based three-dimensional (3D) printing methods, including laser direct-write cell printing and two-photon polymerization, have seen significant advances because of their unique photonic characteristics. Several mechanisms have been developed to increase the overall throughput of two-photon polymerization. Recent efforts to develop complex medically relevant structures using laser direct-write cell printing have also been demonstrated; for example, an ex vivo experimental platform for time-lapse imaging of cancer cell dynamics during angiogenesis within a microvascular network, which combines laser direct-write cell printing into the rat mesentery culture model; a model that simulates a 3D in vivo culture. Laser 3D printing methods hold significant promise for 3D printing of tissue engineering scaffolds, microstructured medical devices, and other medically relevant structures.

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Microneedle-Based Delivery of Amphotericin B for Treatment of Cutaneous Leishmaniasis

Cited by 23 publications

References 43 publications

Topical Amphotericin B Semisolid Dosage Form for Cutaneous Leishmaniasis: Physicochemical Characterization, Ex Vivo Skin Permeation and Biological Activity

Topical Amphotericin B Semisolid Dosage Form for Cutaneous Leishmaniasis: Physicochemical Characterization, Ex Vivo Skin Permeation and Biological Activity

Bioinspired microneedle patches: Biomimetic designs, fabrication, and biomedical applications

Translation of laser-based three-dimensional printing technologies

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