Micromonospora nickelidurans sp. nov., isolated from soil from a nickel-mining site

Abstract

T . This strain harboured meso-diaminopimelic acid, alanine and glycine as the major cell-wall amino acids, xylose and glucose as the characteristic whole-cell sugars, and iso-C 15 : 0 (20.53 %),iso-C 17 : 0 (12.74 %), iso-C 16 : 0 (12.15 %), anteiso-C 17 : 0 (7.97 %), C 17 : 1 v8c (7.49 %) and C 17 : 0 (6.63 %) as the dominant fatty acids. The major menaquinones were MK-10(H 4 ) and MK-10(H 6 ). The phospholipid profile comprised phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol and unknown phosphoglycolipids. The DNA G+C content was 71.4 mol%. A comprehensive analysis of several physiological and biochemical traits and DNA-DNA relatedness indicated that strain K55T was different from closely related species. These phenotypic, genotypic and chemotaxonomic data suggest that strain K55 T represents a novel species of the genus Micromonospora, for which the name Micromonospora nickelidurans sp. nov., is proposed. The type strain is K55 T (5JCM 30559 T 5ACCC19713 T ).

The Response of a 16S Ribosomal RNA Gene Fragment Amplified Community to Lead, Zinc, and Copper Pollution in a Shanghai Field Trial

“…The M. aurantiaca OTU could be separated into two amplicons (sub-OTUs) which varied only by a single nucleotide (position 319), which distinguishes the amplified 16s rRNA gene region submitted from the isolated species M. oryzae , M. halophytica , M. haikouensis , and M. carbonacea from M. aurantiaca , M. coxensis , M. mangrovi , M. purpureochromogenes , and M. wenchangensis . While Micromonospora species, such as M. aurantiaca ( Hamdali et al, 2008 ) and M. nickelidurans ( Lin et al, 2015 ), have been isolated from high metal content soils, this potentially specific tolerance to zinc has not been documented. As the genus is known to contain plant growth promoting bacteria ( Tekaya et al, 2012 ), such as M. carbonacea ( El-Tarabily et al, 2000 ), this increase in abundance may be indicative of secondary interactions with tolerant plant species.…”

Genome-based classification of micromonosporae with a focus on their biotechnological and ecological potential

“…At the time of writing the genus encompasses 79 species with validly published names ( http://www.bacterio.net/micromonospora.html ) 50 , the majority of which have been described using polyphasic methods 44 , 51 though there is evidence that the taxon remains underspeciated 52 , 53 . Initially, micromonosporae were associated with soil, freshwater and marine habitats 44 but novel strains have been isolated from animal 54 – 56 and plant tissues 57 – 64 , as well as from limestone 65 , Antarctic sandstone 66 and from a nickel mining site 67 . Micromonosporae form a tight cluster within the Micromonosporaceae 16S rRNA gene tree 44 , 51 though 16S rRNA gene sequences are not sufficiently divergent to distinguish between closely related strains thereby drawing upon the need for associated DNA-DNA relatedness studies 68 , 69 .…”

Defining the Species Micromonospora saelicesensis and Micromonospora noduli Under the Framework of Genomics

“…The type species of the genus is Micromonospora chalcea and currently includes 81 species with validly published names http://www.bacterio.net/micromonospora.html (Parte, 2014 ). Most of these species have been described in the past 10 years with representative strains isolated from diverse habitats such as soil (Li and Hong, 2016 ; Lee and Whang, 2017 ), aquatic habitats (Trujillo et al, 2005 ; de Menezes et al, 2012 ), plant tissues (Carro et al, 2012 , 2013 ; Kittiwongwattana et al, 2015 ; Trujillo et al, 2015 ; Kaewkla et al, 2017 ) and other environments (Hirsch et al, 2004 ; Nimaichand et al, 2013 ; Lin et al, 2015 ). Recently, a revised classification of the genus Micromonospora based on genome sequence data has been proposed (Carro et al, 2018 ).…”