Micromonospora avicenniae sp. nov., isolated from a root of Avicennia marina

Abstract

Strain 268506(T) was isolated from a root of Avicennia marina collected at mangrove forest in Wengchang, Hainan province, China. The 16S rRNA gene sequence of strain 268506(T) showed the highest similarity with Micromonospora equina Y22(T) (98.8 %) and Micromonospora olivasterospora DSM 43868(T) (98.7 %). In addition, gyrB gene phylogeny clearly showed strain 268506(T) should be assigned to the genus Micromonospora but different from any established Micromonospora species. The predominant menaquinones are MK-9(H8) and MK-9(H6). The major fatty acids are iso-C16:0, iso-C15:0 and anteiso-C17:0. The characteristic whole-cell sugars are xylose, mannose and arabinose. The cell wall contains meso-DAP and glycine. Phosphatidylinositol, diphosphatidylglycerol and phosphatidylethanolamine are the characteristic polar lipids. The DNA G+C content is 70.3 mol%. Some physiological and biochemical properties combined with low DNA-DNA relatedness indicated that the novel strain could be readily distinguished from the closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain 268506(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora avicenniae sp. nov. is proposed. The type strain is 268506(T) ( = CCTCC AA 2012010(T) = DSM 45758(T)).

Genome-Based Taxonomic Classification of the Phylum Actinobacteria

“…The description is as before (Li et al, 2013 ) with the following modification. The G+C content of the type-strain genome is 71.5%, its approximate size 6.82 Mbp, its GenBank deposit SAMN05444858 .…”

Diving Into Reef Ecosystems for Land-Agriculture Solutions: Coral Microbiota Can Alleviate Salt Stress During Germination and Photosynthesis in Terrestrial Plants

“…We also found that the interaction between N. attenuata and S. arenicola resembled that of plant-endophytic symbiosis ( Figure 5A). Other actinobacteria (i.e., Micromonospora and Verrucosispora) from the same family of the coral symbiont Salinispora have been isolated from the root nodules of terrestrial and marine plants where they presented endophytic interactions and conferred advantages to their plant hosts (Figure 2; Li et al, 2013;Ngaemthao et al, 2017;Kuncharoen et al, 2018). Bioinformatic analysis suggests that Salinispora spp.…”

Micromonospora zhanjiangensis sp. nov., isolated from mangrove forest soil

“…The morphological characteristics of strain 2902at01 T were assessed by scanning electron microscopy (TM 3000; Hitachi) using 7-day-old cultures on ISP 2 agar. Cultural characteristics of strain 2902at01 T were determined on various agar media including ATCC 172 agar, Czapek's agar, ISP 1–7 agar, MB agar, and potato-glucose agar as previously used (Li et al , 2013a), and M8 agar (Castiglione et al , 2008) and nutrient agar (Waksman, 1967). The ISCC-NBS colour chart was used to determine the names and designations of colony colours (Kelly, 1964).…”

“…Analysis of phospholipids was carried out using TLC according to Minnikin et al (1984). Fatty acids were extracted from fresh cells obtained with the same cultural conditions above using the method of Sasser (1990) and the composition was determined by GC-MS performed on a GC instrument (Li et al 2013a). Menaquinones were extracted from freeze-dried biomass, purified according to Minnikin et al (1984) and finally analysed by HPLC with an ODS-BP C18 column (4.66250 mm); the elution solvent was methanol and 2-propanol (3 : 2, v/v) based on the method of Li et al (2013a).…”