Micromolding of polymers

Yao, Donggang

doi:10.1533/9781845696429.4.552

Cited by 4 publications

(2 citation statements)

References 79 publications

(70 reference statements)

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“…The negative mould, which is used to transfer a pattern onto a surface for micro moulding, is often fabricated using micromachining techniques. Next to lithographic methods, replication methods and material removal methods can be used to create the moulds ( Yao, 2009 ). Additive manufacturing techniques have also been used to create moulds that can then be used to fabricate larger batches by replication.…”

Section: Fabrication Techniques and Materials For Scaffold-based 3d C...mentioning

confidence: 99%

Engineered cell culture microenvironments for mechanobiology studies of brain neural cells

Ransanz

Altena²,

Heine³

et al. 2022

Front. Bioeng. Biotechnol.

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The biomechanical properties of the brain microenvironment, which is composed of different neural cell types, the extracellular matrix, and blood vessels, are critical for normal brain development and neural functioning. Stiffness, viscoelasticity and spatial organization of brain tissue modulate proliferation, migration, differentiation, and cell function. However, the mechanical aspects of the neural microenvironment are largely ignored in current cell culture systems. Considering the high promises of human induced pluripotent stem cell- (iPSC-) based models for disease modelling and new treatment development, and in light of the physiological relevance of neuromechanobiological features, applications of in vitro engineered neuronal microenvironments should be explored thoroughly to develop more representative in vitro brain models. In this context, recently developed biomaterials in combination with micro- and nanofabrication techniques 1) allow investigating how mechanical properties affect neural cell development and functioning; 2) enable optimal cell microenvironment engineering strategies to advance neural cell models; and 3) provide a quantitative tool to assess changes in the neuromechanobiological properties of the brain microenvironment induced by pathology. In this review, we discuss the biological and engineering aspects involved in studying neuromechanobiology within scaffold-free and scaffold-based 2D and 3D iPSC-based brain models and approaches employing primary lineages (neural/glial), cell lines and other stem cells. Finally, we discuss future experimental directions of engineered microenvironments in neuroscience.

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Section: Fabrication Techniques and Materials For Scaffold-based 3d C...mentioning

confidence: 99%

Engineered cell culture microenvironments for mechanobiology studies of brain neural cells

Ransanz

Altena²,

Heine³

et al. 2022

Front. Bioeng. Biotechnol.

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“…Figure 2 shows the schematic diagram with the actual dimensions of the lab-on-a-chip, it also shows the integrated electrodes for supplying voltages for the detection of dopamine and other chemicals. Microchannels in PDMS were fabricated using micro molding process [9]. Silicon wafer with patterned with photoresist was used as a mold master to have a relatively thick structure of microchannels for transporting samples and reagents.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Nanoparticle Based Lab-On-A-Chip Devices for the Detection of Dopamine

2023

JRAR

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Dopamine is a neurotransmitter compound that is produced in the substantial ventral typical area and hypothalamus of the brain. Dysfunction of the dopamine system has been implicated in different nervous systems. A novel integrated Lab-on-achip device using PDMS and glass plate has been successfully designed, fabricated, and tested for various concentrations of dopamine. For the separation of dopamine and ascorbic acid mixture, the working electrode of the Lab-on-a-Chip device was modified by coating with Au- nanocomposite particles, which separates dopamine and ascorbic acid.

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