Micromolar concentrations of Al3+ induce phase separation, aggregation and dye release in phosphatidylserine-containing lipid vesicles

Deleers, Michel; Servais, Jean-Paul; Wülfert, Ernst

doi:10.1016/0005-2736(85)90233-0

Cited by 76 publications

(24 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5). Although several reports have focused on the physical changes of artificial liposomes and PM (Deleers et al 1985;Suhayda and Haug 1986;Zhao et al 1987;Chen et al 1991), this is the first evidence suggesting the existence of an early strategy for A1 tolerance that is regulated by PM lipid. As the intact PM lipid bilayers differ in the com-position between inner and outer leaflet, the present permeability result can be accepted as the average characteristics of the reconstituted homogenous lipid bilayers of PM.…”

Section: Resultsmentioning

confidence: 96%

“…We established a short-term A1 tolerance screening technique that is useful for the real time identification of the induction of differential A1 tolerance among plant species. Although several reports have focused on the significance of PM lipid in A1 tolerance (Deleers et al 1985;Zhang et al 1996;Yermiyahu et al 1997;Ishikawa and Wagatsuma 1998;Ishikawa et al 2001), there has not yet been any direct demonstration. Liposomes and synthetic lipid bilayer vesicles frequently utilized for permeability experiments have several disadvantages, such as a very small inner aqueous phase, size distribution and fragility (Okahata and Seki 1984).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Plasma Membrane Lipids Are the Powerful Components for Early Stage Aluminum Tolerance in Triticale

Wagatsuma¹,

Ishikawa²,

Uemura³

et al. 2005

Soil Science and Plant Nutrition

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 96%

mentioning

confidence: 99%

Plasma Membrane Lipids Are the Powerful Components for Early Stage Aluminum Tolerance in Triticale

Wagatsuma¹,

Ishikawa²,

Uemura³

et al. 2005

Soil Science and Plant Nutrition

View full text Add to dashboard Cite

“…In recent years biophysical work (Deleers et al 1985(Deleers et al , 1986) has shown that AI(III) at micromolar concentrations induces phase separation, aggregation, dye release and membrane rigidiflcation in phosphatidylserine-and phosphatdylethanolamine-containing lipid vesicles.…”

Section: Ai(iii) Is An Established Neurotoxinmentioning

confidence: 99%

Reactivity of Al(III) with membrane phospholipids: a NMR approach

Zambenedetti¹,

Tisato²,

Corain³

et al. 1994

Biometals

View full text Add to dashboard Cite

The complexes Al(acac)3 (1) (acac = 2,4-pentanedionate) and Al(malt)3 (malt = 3-hydroxy-2-methyl-4-pyronate) (2) react with DL-alpha-dipalmitoylphosphatidylcholine (DPPC) under a 1:1 molar ratio in CDCl3 at 37 degrees C, as shown by the substantial release of ligands (20-50%) from the metal coordination sphere (1H-NMR), by evident changes in the 1H-NMR spectrum of DPPC in the reaction mixture and by the appearance of a 31P-NMR signal due to metal-coordinated DPPC. 31P-NMR spectra reveal that both 1 and 2 also react with DPPC in water, in the presence of 1% Triton X-100 and Tris buffer. Under these conditions, 1 and 2 do not react with ghosts from human erythrocytes. On the contrary, the far less hydrolytically stable complex Al(lact)3 (lact = lactate) appears to be reactive under identical conditions, as shown by 31P-NMR spectra.

show abstract

“…Studies on membrane fusion have been performed mainly on liposomes made with acidic phospholipids, secretory vesicles and viruses (Ktappe, Wilschut & Hoekstra, 1986;Ekerdt, Dahl & Gratzl, 1981) and particular relevance has been given to the role of ions (Ohki & Ohshima, 1985;Deleers, Servais & Wfilfert, 1985). To this purpose, it should be noticed that Ca 2+ is certainly involved in fusion (Chernomordik, Melikyan & Chizmadzhev, 1987), but the role of Mg 2+ and of other cations is still under debate (Chernomordik et al, 1987;Yoshimura & Aki, 1985).…”

Section: Introductionmentioning

confidence: 98%

Fusion of liposomes and rat brain microsomes examined by two assays

et al. 1989

View full text Add to dashboard Cite

Liposomes are prepared from rat brain microsomal lipid and loaded with either Tb3+ or dipicolinic acid (DPA) to test fusion with the Tb-DPA assay. They are also loaded with octadecyl Rhodamine B chloride (R18) to test fusion with the R18 assay. The addition of either Ca2+ or Mg2+ to loaded liposomes develops fluorescence with both assays. The fluorescence elicited by Mg2+ is similar to that elicited by Ca2+ if assessed with R18, but much higher if determined by Tb-DPA. The Ca2(+)-dependent fluorescence of the Tb-DPA complex is not suppressed by the addition of EDTA, and therefore it is internal to vesicles. The contrary is true for the Mg2(+)-dependent fluorescence. Rat brain microsomes can be disrupted by adding octylgucoside and reconstituted by removing it by dialysis. We use this procedure to load microsomes with DPA. This allows the use of the Tb-DPA assay for testing the fusion of rat brain microsomes. Reconstituted microsomes fuse with liposomes. This fusion has characteristics similar to those of liposome-liposome fusion. However, no microsome-microsome fusion could be detected with either method. The two methods give different results, owing to the chemical properties of the assays. Indeed Tb-DPA implies the retention of vesicle content, whereas this is not required by the R18 assay.

show abstract

Micromolar concentrations of Al3+ induce phase separation, aggregation and dye release in phosphatidylserine-containing lipid vesicles

Cited by 76 publications

References 18 publications

Plasma Membrane Lipids Are the Powerful Components for Early Stage Aluminum Tolerance in Triticale

Plasma Membrane Lipids Are the Powerful Components for Early Stage Aluminum Tolerance in Triticale

Reactivity of Al(III) with membrane phospholipids: a NMR approach

Fusion of liposomes and rat brain microsomes examined by two assays

Contact Info

Product

Resources

About