Micrometric size measurement of biological samples using a simple and non-invasive transmission interferometric set up

Frausto-Rea, Gloria; Torre, Manuel H De La; Flores, J. Mauricio; Silva, Luis; Briones-R., Manuel de J.; Santoyo, Fernando Mendoza

doi:10.1364/oe.27.026251

Cited by 7 publications

(3 citation statements)

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“…In the micrometric size inspection, new configurations have been developed in order to simplify the alignment and measurement of these samples. Reference [67] presents a novel transmission DHI set up able to identify micrometric size particles < 50 μm. The optical phase is able to reconstruct pollen particles as 24a shows, while Fig.…”

Section: Digital Holographic Microscopy (Dhm)mentioning

confidence: 99%

“…25 compares the image retrieved with confocal microscopy with the one from transmission DHI for the same pollen particles. It is notorious the advantage of DHI in transmission (using the simplified microscopy set up in reference [67], which renders the microscopic image of the particles and retrieves their profile.…”

Section: Digital Holographic Microscopy (Dhm)mentioning

confidence: 99%

“…Fig.25compares the image retrieved with confocal microscopy with the one from transmission DHI for the same pollen particles. It is notorious the advantage of DHI in transmission (using the simplified microscopy set up in reference[67], which renders the microscopic image of the particles and retrieves their profile.When a phase object is illuminated its transparency or translucent nature does not provoke a large amplitude variation of the transmitted wavefront, which is not the case for phase changes. In these applications, the optical path difference is a consequence of a change in the refractive index distribution along the optical path through the sample.…”

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confidence: 99%

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Macro to nano specimen measurements using photons and electrons with digital holographic interferometry: a review

Hernández-Montes

Mendoza-Santoyo

Moreno

et al. 2020

J. Eur. Opt. Soc.-Rapid Publ.

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Today digital holographic interferometry (DHI) is considered a modern full-field non-destructive technique that allows generating 3D quantitative data of a wide variety of specimens. There are diverse optical setups for DHI that enable the study of specimens in static and dynamic conditions: it is a viable alternative to characterize a wide diversity of parameters in the micro and macro world by conducting repeatable, reliable and accurate measurements that render specimen data, e.g., displacements, shape, spatial dimensions, physiological conditions, refractive indices, and vibration responses. This paper presents a review and progress on the most significant topics, contributions and applications involving DHI for the study of different specimens such as: cells, bio tissues, grains, insects, and nano-structures. For most of the research work involving macro and micro specimens the wave-like source used in the measurements were photons from a laser, while the studies carried out in the nano regime used the wave-like nature of the electron.

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Section: Digital Holographic Microscopy (Dhm)mentioning

confidence: 99%

Section: Digital Holographic Microscopy (Dhm)mentioning

confidence: 99%

mentioning

confidence: 99%

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