Micromethod for Assaying Serum Levels of Erythromycin

Bell, Stanley C.; Hamman, Jack W; Grundy, Walton E.

doi:10.1128/aem.17.1.88-92.1969

Cited by 32 publications

(3 citation statements)

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“…Antibiotic assay for erythromycin.-We used a microbiological assay modified after Bell et al (1969) and Evelyn et al (1986) to determine the concentrations of erythromycin in samples of tissue, plasma, and feed. This method enabled the concentration of antibiotic to be estimated by comparison with the diameters of inhibition zones formed in colonies of an erythromycin-sensitive bacterium by known concentrations of erythromycin in standards.…”

Section: Methodsmentioning

confidence: 99%

Accumulation and Depletion of Orally Administered Erythromycin Thiocyanate in Tissues of Chinook Salmon

Schreck

1988

Transactions of the American Fisheries Society

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Juvenile chinook salmon Oncorhynchus tshawytscha were fed erythromycin thiocyanate for 21 d at a daily therapeutic dosage of either 0.05 or 0.1 g erythromycin/kg body weight. We analyzed samples of liver, spleen, kidney, red muscle, white muscle, and plasma removed from fish at intervals during and after drug administration to determine the concentration of erythromycin present. On all but one sampling date, the kidney had the highest mean concentration (weight of drug per unit of tissue weight) among the tissues sampled. The mean concentrations in all tissues and plasma of fish fed at the daily dosage of 0.05 g/kg did not differ significantly over time while the drug was fed. However, erythromycin accumulated over time in the liver, spleen, kidney, red muscle, and white muscle offish fed at 0.1 g/kg; concentrations of the drug peaked on the last day of antibiotic administration. Doubling the dosage from 0.05 to 0.1 g/kg resulted in a 4-10-fold increase in erythromycin concentration in tissues, and a twofold increase in plasma on the last day of feeding. The patterns of depletion of erythromycin in tissues and plasma of chinook salmon fed either drug-treated ration were similar. Small quantities of erythromycin were detectable in the liver and kidney 19 d after drug administration ceased, but not in the muscle.

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Section: Methodsmentioning

confidence: 99%

Accumulation and Depletion of Orally Administered Erythromycin Thiocyanate in Tissues of Chinook Salmon

Schreck

1988

Transactions of the American Fisheries Society

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“…The precision attained with the use of disposable micropipets appeared to be less dependent upon the technical expertise of the operator than did methods involving larger pipets, capillary action, or dipping the discs into serum. The precision compares favorably with the precision of a micromethod carried out on small petri dishes, as recently described for the assay of colicins 7and for the assay of serum levels of erythromycin (1).…”

Section: Reslmtsmentioning

confidence: 75%

Large-Plate Method for the Assay of Neomycin in Serum

Soestbergen¹

1969

Applied Microbiology

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A large-plate method employing radial diffusion from small paper discs for assaying serum levels of neomycin is described. More than 120 discs placed on a loading plate and loaded with 20 Mliters of sample could all be brought into contact with the agar plate at one time. The requirement for elaborate statistical design to compensate for time-dependent bias was thus eliminated. The dose-response curve was linear for a range of at least 0.5 to 2.5 jAg/ml. The experimental limits for

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“…1) is widely used as a macrolide antibiotic. Previous pharmacokinetic data published on this antibiotic have been based predominantly on a microbiological method (Bell et al, 1969). Several high-performance liquid chromatography (HPLC) methods with UV (Yamashita et al, 1993;Cachet et al, 1992;Stubbs et al, 1985), electrochemical detection (Janecek et al, 1993;Laakso et al, 1990;Grgurinovich and Matthews, 1988;Stubbs and Kanfer, 1988;Nilsson et al, 1987;Croteau et al, 1987;Chen and Chiou, 1983), fluorimetric detection (Tod et al, 1992;Tsuji, 1978) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) have been developed for the determination of EA in biological fluids (Li et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Determination of erythromycin A in rat plasma and urine by high‐performance liquid chromatography with chemiluminescence detection using Tris(2,2′‐bipyridine)ruthenium(II)

Hori

Hashimoto

Konishi

2006

Biomedical Chromatography

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A simple and sensitive method was developed for the determination of erythromycin A (EA), decladinosyl erythromycin A (dClEA) and erythromycin B (EB) in rat plasma and urine by high-performance liquid chromatography with electrogenerated chemiluminescence detection using Tris(2,2'-bipyridine)ruthenium(II). The recovery rates of EA, dClEA and EB were 97, 94 and 85% from rat plasma and 89, 83 and 93% from rat urine, respectively. The calibration curves were linear over the concentration ranges 0.05-5 microg/mL for plasma and 0.5-50 microg/mL for urine. The precision and accuracy for all analytes in rat plasma were < or =9.0 and -6.3-7.2%, and those in urine were < or =9.4% and -6.1-7.6%, respectively. This method proved to be a powerful tool for determination of EA, dClEA and EB concentrations in samples from rats.

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Micromethod for Assaying Serum Levels of Erythromycin

Cited by 32 publications

References 1 publication

Accumulation and Depletion of Orally Administered Erythromycin Thiocyanate in Tissues of Chinook Salmon

Accumulation and Depletion of Orally Administered Erythromycin Thiocyanate in Tissues of Chinook Salmon

Large-Plate Method for the Assay of Neomycin in Serum

Determination of erythromycin A in rat plasma and urine by high‐performance liquid chromatography with chemiluminescence detection using Tris(2,2′‐bipyridine)ruthenium(II)

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