Micromechanical observation of the kinetics of biomolecular interactions

Kwon, Taeyun; Eom, Kilho; Park, Jinsung; Yoon, Dae Sung; Lee, Hong Lim; Kim, Tae Song

doi:10.1063/1.3006329

Cited by 38 publications

(27 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Until now, with few exceptions [22,23,172], there have been very few studies on in situ detection of proteins in aqueous environments. Here, in situ detection of proteins in solution is of high significance, because in situ detection allows the real-time monitoring of protein-protein interactions, which will enable novel insights into the kinetics of protein-protein interactions.…”

Section: Protein Detectionmentioning

confidence: 99%

“…For instance, when a microcantilever acting as an AFM tip vibrates in an aqueous environment in order to image a biological sample in an aqueous environment [89,90], the hydrodynamic effect significantly deteriorates the resonance behavior of microcantilever, and consequently restricts the resolution of the AFM image. Furthermore, cantilever sensors have recently been employed for in situ detection of biological molecules and/or biomolecular interactions in an aqueous environment [22,23,26,45,91], where the hydrodynamic effect significantly reduces the resonant frequencies, which consequently reduces the detection sensitivity. The hydrodynamic effect also has a significant impact on the resonance behavior of NEMS in air.…”

Section: Resonance Behavior In An Aqueous Environmentmentioning

confidence: 99%

“…For instance, micro/nano-electro-mechanical system (MEMS/NEMS) devices have allowed the sensitive detection of physical quantities such as spin [4,5], molecular mass [6][7][8][9][10], quantum state [11] (see also Refs. [12][13][14] which discuss the prospect of NEMS for studying quantum mechanics), thermal fluctuation [15][16][17][18], coupled resonance [19][20][21], and biochemical reactions [22][23][24][25][26]. Among MEMS/NEMS devices, nanomechanical resonators have been recently highlighted for their unprecedented dynamic characteristics as they can easily reach ultra-high-frequency (UHF) and/or very-high-frequency (VHF) dynamic behavior up to the Giga Hertz (GHz = 10 9 Hz) regime [3,[27][28][29].…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, micro/nano-mechanical resonators are able to easily overcome the "diagnostic gray zone" limitation because of their unprecedented detection sensitivity even at single-molecule resolution [9,10,44], which shows that nanomechanical resonators can serve as lab-on-a-chip biosensors enabling the early diagnostics of important diseases such as cancer. Moreover, nano/micro-mechanical resonators show the promising ability to provide the detailed mechanisms of biochemical reactions [23,24,26,45,46] and/or cell functions [47].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nanomechanical resonators and their applications in biological/chemical detection: Nanomechanics principles

et al. 2011

Self Cite

View full text Add to dashboard Cite

Recent advances in nanotechnology have led to the development of nano-electro-mechanical systems (NEMS) such as nanomechanical resonators, which have recently received significant attention from the scientific community. This has not only been for their capability for the label-free detection of bio/chemical-molecules at single-molecule (or atomic) resolution for future applications such as the early diagnostics of diseases such as cancer, but also for their unprecedented ability to detect physical quantities such as molecular weight, elastic stiffness, surface stress, and surface elastic stiffness for adsorbed molecules on the surface. Most experimental works on resonator-based molecular detection have been based on the principle that molecular adsorption onto a resonator surface increases the effective mass, and consequently decreases the resonant frequencies of the nanomechanical resonator. However, this principle is insufficient to provide fundamental insights into resonator-based molecular detection at the nanoscale; this is due to recently proposed novel nanoscale detection principles including various effects such as surface effects, nonlinear oscillations, coupled resonance, and stiffness effects. Furthermore, these effects have only recently been incorporated into existing physical models for resonators, and therefore the universal physical principles governing nanoresonator-based detection have not been completely described. Therefore, our objective in this review is to overview the current attempts to understand the underlying mechanisms in nanoresonator-based detection using physical models coupled to computational simulations and/or experiments. Specifically, we will focus on issues of special relevance to the dynamic behavior of nanoresonators and their applications in biological/chemical detection: the resonance behavior of micro/nano-resonators; resonator-based chemical/biological detection; physical models of various nanoresonators such as nanowires, carbon nanotubes, and graphene. We pay particular attention to experimental and computational approaches that have been useful in elucidating the mechanisms underlying the dynamic behavior of resonators across multiple and disparate spatial/length scales, and the resulting insight into resonator-based detection that has been obtained. We additionally provide extensive discussion regarding potentially fruitful future research directions coupling experiments and simulations in order to develop a fundamental understanding of the basic physical principles that govern NEMS and NEMS-based sensing and detection applications.

show abstract

Section: Protein Detectionmentioning

confidence: 99%

Section: Resonance Behavior In An Aqueous Environmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nanomechanical resonators and their applications in biological/chemical detection: Nanomechanics principles

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…An array of microfabricated biosensors represents a promising alternative to detect low concentrations of biomolecules in real time. 3 Micromechanical resonators including a microcantilever, [4][5][6][7][8][9][10][11] quartz crystal microbalance (QCM), [12][13][14][15][16] and surface acoustic wave (SAW) sensors [17][18][19] measure the resonance frequency shift associated with the loaded mass by selective trapping of target molecules. These methods exhibit high massresolution in air or vacuum owing to their high quality factor and have superior sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Application of a new microcantilever biosensor resonating at the air–liquid interface for direct insulin detection and continuous monitoring of enzymatic reactions

et al. 2012

View full text Add to dashboard Cite

Here we describe the application of a recently developed high-resolution microcantilever biosensor resonating at the air-liquid interface for the continuous detection of antigen-antibody and enzymesubstrate interactions. The cantilever at the air-liquid interface demonstrated 50% higher quality factor and a 5.7-fold increase in signal-to-noise-ratio (SNR) compared with one immersed in the purified water. First, a label-free detection of a low molecular weight protein (insulin, 5.8 kDa) in physiological concentration was demonstrated. The liquid facing side of the cantilever was functionalized by coating its surface with insulin antibodies, while the opposite side was exposed to air. The meniscus membrane at the micro-slit around the cantilever sustained the liquid in the microchannel. After optimizing the process of surface functionalization, the resonance frequency shift was successfully measured for insulin solutions of 0.4, 2.0, and 6.3 ng ml 21. To demonstrate additional application of the device for monitoring enzymatic protein degradation, the liquid facing microcantilever surface was coated with human recombinant SOD1 (superoxide dismutase 1) and exposed to various concentrations of proteinase K solution, and the kinetics of the SOD1 digestion was continuously monitored. The results showed that it is a suitable tool for sensitive protein detection and analysis.

show abstract

Real‐Time Quantitative Monitoring of Specific Peptide Cleavage by a Proteinase for Cancer Diagnosis

Lee¹,

Eom²,

Park³

et al. 2012

Angewandte Chemie

Self Cite

View full text Add to dashboard Cite

Gute Schwingungen: Ein resonanter Massesensor, auf dessen Oberfläche Peptide immobilisiert sind, wird in einem Bioassay verwendet, mit dem die proteolytische Aktivität der Membran‐Typ‐1‐Matrix‐Metalloproteinase (MT1‐MMP) bestimmt werden kann. Dabei werden Änderungen in der Frequenz des Massesensors gemessen, die nach der spezifischen proteolytischen Spaltung der immobilisierten Zielpeptide durch MT1‐MMP auftreten (siehe Bild).

show abstract

Micromechanical observation of the kinetics of biomolecular interactions

Cited by 38 publications

References 19 publications

Nanomechanical resonators and their applications in biological/chemical detection: Nanomechanics principles

Nanomechanical resonators and their applications in biological/chemical detection: Nanomechanics principles

Application of a new microcantilever biosensor resonating at the air–liquid interface for direct insulin detection and continuous monitoring of enzymatic reactions

Real‐Time Quantitative Monitoring of Specific Peptide Cleavage by a Proteinase for Cancer Diagnosis

Contact Info

Product

Resources

About