Micromanipulation of Langmuir-Monolayers with Optical Tweezers

Wurlitzer, S.; Lautz, C.; Liley, Martha; Duschl, Claus; Fischer, Thomas M.

doi:10.1021/jp0024266

Cited by 55 publications

(52 citation statements)

References 22 publications

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“…Given the previous measurements of the temperature rise in the 1064 nm continuous wave laser trap, and their corresponding range of the in situ powers and focal power densities2122232425, the temperature rise in our experiments is in the order of ≈1°C/100 mW in the focal volume. Such a temperature rise would be expected to have little, if any, biological consequence.…”

Section: Discussionsupporting

confidence: 56%

Determination of motility forces on isolated chromosomes with laser tweezers

Khatibzadeh

Stilgoe

Bui

et al. 2014

Sci Rep

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Quantitative determination of the motility forces of chromosomes during cell division is fundamental to understanding a process that is universal among eukaryotic organisms. Using an optical tweezers system, isolated mammalian chromosomes were held in a 1064 nm laser trap. The minimum force required to move a single chromosome was determined to be ≈0.8–5 pN. The maximum transverse trapping efficiency of the isolated chromosomes was calculated as ≈0.01–0.02. These results confirm theoretical force calculations of ≈0.1–12 pN to move a chromosome on the mitotic or meiotic spindle. The verification of these results was carried out by calibration of the optical tweezers when trapping microspheres with a diameter of 4.5–15 µm in media with 1–7 cP viscosity. The results of the chromosome and microsphere trapping experiments agree with optical models developed to simulate trapping of cylindrical and spherical specimens.

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Section: Discussionsupporting

confidence: 56%

Determination of motility forces on isolated chromosomes with laser tweezers

Khatibzadeh

Stilgoe

Bui

et al. 2014

Sci Rep

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“…1͒. With a combination of optical tweezers and fluorescence microscopy 13 we simultaneously manipulate and visualize a Langmuir monolayer of methyl-octadecanoate. A single liquid condensed domain is forced to undergo combined translational and rotational motion.…”

Section: Introductionmentioning

confidence: 99%

The translational and rotational drag on Langmuir monolayer domains

Steffen

Heinig

Wurlitzer

et al. 2001

The Journal of Chemical Physics

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A technique for direct observation of particle motion under shear in a Langmuir monolayerThe ratio of the rotational and translational drag coefficient of a circular liquid condensed Langmuir monolayer domain moved in different phases is measured. A single domain is fixed at its boundary and forced to undergo combined translational and rotational motion. It is observed, that the drag force is dominated by the viscous dissipation of the three dimensional subphase and affected by the elasticity of the surrounding monolayer phase.

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“…Recent studies have shown that the temperature rise was 10-20 T (degree)/W on focusing a sub-W trapping laser in water [41][42][43]. In the case of our higher laser power (270 mW), the temperature rise is estimated to be ≈ 6 K at most, thus not reaching to the transition temperature.…”

Section: Phase Transition Dynamicsmentioning

confidence: 73%

Photon Force-Induced Phase Transition Dynamics of Single Hydrogel Nanoparticles in Water

et al. 2007

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We investigated laser-induced phase transition dynamics of µm sized single poly(N -isopropylacrylamide) hydrogel particles in water. Single poly(N --isopropylacrylamide) gel particles labeled with a polarity-sensitive fluorescent probe monomer were optically trapped by a focused laser beam and its fluorescence dynamics was analyzed. The fluorescence intensity of trapped single gel particles was increased with the trapping laser power, while the fluorescence peak wavelength was not changed. Temperature-induced changes of fluorescence properties of the single particle were confirmed to be similar to those of the bulk solution. These behaviors are well interpreted by considering that the fluorescence intensity and fluorescence peak reflect local interactions between the fluorescent probe and attaching (bound) water molecules and effective polarity determined by (free) water content in the particle, respectively. A change in the fluorescence peak wavelength after laser trapping was followed and its blue-shift was confirmed to occur within a few hundreds seconds, indicating that a single gel particle gradually attains to a globular state on this timescale, expelling initially free water molecules and then bound ones.

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Micromanipulation of Langmuir-Monolayers with Optical Tweezers

Cited by 55 publications

References 22 publications

Determination of motility forces on isolated chromosomes with laser tweezers

Determination of motility forces on isolated chromosomes with laser tweezers

The translational and rotational drag on Langmuir monolayer domains

Photon Force-Induced Phase Transition Dynamics of Single Hydrogel Nanoparticles in Water

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