Micromanipulation

Abstract

Micromanipulation technology entered the forum of human conception in vitro in the late 1980s. It was erroneously perceived as a new technology – a purely mechanical approach to bypass failures of conception in vitro, the aetiology of which were unknown. In fact, it is the modification of technology developed since the beginning of this century, and its logical utilization for conception in vitro, preconception (polar body) biopsy and preimplantation (blastomere and trophectoderm) biopsy in the realm of human infertility and genetic disorders.

Marvellous ICSI: the viewpoint of a clinician

“…Initial attempts to inject spermatozoa into the ooplasm were largely unsuccessful ( Fishel et al ., 1993 ; Lacham‐Kaplan & Trounson, 1995), and there developed a feeling that the technique would not be successful in man. Other procedures to improve fertilization rates with oligoasthenotratatozoospermia (OAT) were developed, including zona opening, partial zona dissection (PZD) and subzonal insemination (SUZI), which allowed spermatozoa access to the oolemma ( Odawara & Lopata, 1989; Fishel et al ., 1993 ). The results with these techniques in humans were generally poor with only about 20% of oocytes fertilizing normally, and there were few pregnancies.…”

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“…In addition, standard way and used for injection, they are less successful than those used with forceful immobilization a significant difference was not observed in the proportion of parthenogenetically activated oocytes and (13). It has been established that ICSI with sperm immobilization yields higher fertilization rates (14,1,15) and oocytes displaying three pronuclei among the four groups. The mean proportion of single-pronuclear and that external mechanical pressure increases permeability (1).…”