Micromachined analytical devices: microchips for semen testing

Kricka, Larry J.; Faro, Ilcedes; Heyner, Susan; Garside, William T.; Fitzpatrick, Glen; McKinnon, Graham; Ho, John W.; Wilding, Peter

doi:10.1016/s0731-7085(96)02046-8

Cited by 58 publications

(46 citation statements)

References 19 publications

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“…Microfluidics has shown potential in assisted reproductive technology (ART) because of its rapid analysis capability on small sample volumes and the potential to reduce manual operation (15)(16)(17). Several microfluidic devices have been developed for sperm testing (15,16 ).…”

mentioning

confidence: 99%

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Integration of Sperm Motility and Chemotaxis Screening with a Microchannel-Based Device

Xie

Han

et al. 2010

Clinical Chemistry

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BACKGROUND Sperm screening is an essential step in in vitro fertilization (IVF) procedures. The swim-up method, an assay for sperm motility, is used clinically to select the ideal sperm for subsequent manipulation. However, additional parameters, including acrosome reaction capability, chemotaxis, and thermotaxis, are also important indicators of mammalian sperm health. To monitor both sperm motility and chemotaxis simultaneously during sperm screening, we designed and constructed a microdevice comprising a straight channel connected with a bibranch channel that mimics the mammalian female reproductive tract. METHODS The width and length of the straight channel were optimized to select the motile sperms. We selectively cultured cumulus cells in the bibranch channel to generate a chemoattractant-forming chemical gradient. Sperm chemotaxis was represented by the ratio of the sperm swimming toward different branches. RESULTS The percentage of motile sperms improved from 58.5% (3.8%) to 82.6% (2.9%) by a straight channel 7 mm in length and 1 mm in width. About 10% of sperms were found to be chemotactically responsive in our experiment, which is consistent with previous studies. CONCLUSIONS For the first time, we achieved the combined evaluation of both sperm motility and chemotaxis. The motile and chemotactically responsive sperms can easily be enriched on a lab-on-a-chip device to improve IVF outcome.

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confidence: 99%

“…Several microfluidic devices have been developed for sperm testing (15,16 ). Kricka and colleagues (15,16 ) developed a motile spermtesting microchip with tortuous channels. A microfluidic device based on sperm-sorting method was proposed by Cho et al in 2003 (17 ) that used a parallel laminar flow stream to distinguish motile from poorquality sperms.…”

mentioning

confidence: 99%

Integration of Sperm Motility and Chemotaxis Screening with a Microchannel-Based Device

Xie

Han

et al. 2010

Clinical Chemistry

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“…Classically, sperm processing for IVF has been performed with simple media washing, semen overlay with medium and swim-up of sperm out of the seminal plasma, density gradient centrifugation or a combination of above methods (WHO, 2010). In the early-to mid-1990s, Kricka et al (1993Kricka et al ( , 1997 made and tested silicon/glass microfluidic devices as diagnostic tools for evaluating sperm motility. These investigators assessed sperm progression through branching microchannels with a suggestion that such a microfluidic device could displace conventional methods of motility testing in clinical semen analyses.…”

Section: Spermmentioning

confidence: 99%

Application of microfluidic technologies to human assisted reproduction

Smith

Takayama

2017

Mol. Hum. Reprod.

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Microfluidics can be considered both a science and a technology. It is defined as the study of fluid behavior at a sub-microliter level and the investigation into its application to cell biology, chemistry, genetics, molecular biology and medicine. There are at least two characteristics of microfluidics, mechanical and biochemical, which can be influential in the field of mammalian gamete and preimplantation embryo biology. These microfluidic characteristics can assist in basic biological studies on sperm, oocyte and preimplantation embryo structure, function and environment. The mechanical and biochemical characteristics of microfluidics may also have practical and/or technical application(s) to assisted reproductive technologies (ART) in rodents, domestic species, endangered species and humans. This review will consider data in mammals, and when available humans, addressing the potential application(s) of microfluidics to assisted reproduction. There are numerous sequential steps in the clinical assisted reproductive laboratory process that work, yet could be improved. Cause and effect relations of procedural inefficiencies can be difficult to identify and/or remedy. Data will be presented that consider microfluidic applications to sperm isolation, oocyte cumulus complex isolation, oocyte denuding, oocyte mechanical manipulation, conventional insemination, intracytoplasmic sperm injection, embryo culture, embryo analysis and oocyte and embryo cryopreservation. While these studies have progressed in animal models, data with human gametes and embryos are significantly lacking. These data from clinical trials are requisite for making future evidence-based decisions regarding the application of microfluidics in human ART.

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“…These microfluidic devices can be used by pipetting sample liquid without complex fluid manipulation systems, such as electric pumps and bulbs, leading to the advantages of simplicity and low cost. Since the 1990s, microfluidic devices for sperm counts and motility analyses have been investigated by Kricka et al [39], and microfluidic POC systems for semen diagnostics sorting motile sperms or on-chip IVF using LOC have been reported [40][41][42][43][44][45][46][47][48][49][50][51]. Moreover, in addition to motile sperm separation, fluid flow-and viscosity-associated chemotaxis and mechanotaxis of mammalian sperm were investigated using microfluidic channels [45].…”

Section: Microfluidic Channel-based Devices For Semen Diagnoses and Mmentioning

confidence: 99%

Recent advances in microfluidic diagnostic and treatment systems for assisted reproductive technologies in developing countries

Matsuura¹,

Nishina²,

Asano³

2017

Point-of-Care Diagnostics - New Progresses and Perspectives

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Micromachined analytical devices: microchips for semen testing

Cited by 58 publications

References 19 publications

Integration of Sperm Motility and Chemotaxis Screening with a Microchannel-Based Device

Integration of Sperm Motility and Chemotaxis Screening with a Microchannel-Based Device

Application of microfluidic technologies to human assisted reproduction

Recent advances in microfluidic diagnostic and treatment systems for assisted reproductive technologies in developing countries

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