Microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self‐etching primers/adhesives

Amend, Stefanie; Frankenberger, Roland; Boutsiouki, Christina; Scharrelmann, Vanessa; Winter, Julia; Krämer, Norbert

doi:10.1002/cre2.420

Cited by 8 publications

(8 citation statements)

References 26 publications

(37 reference statements)

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“…(42) The methylene blue dye has several features as it's cost-effective, easy to handle, has a high degree of staining, and has a smaller molecular weight than bacterial toxins. (43,44) The ISO 40494 three-point bending test is used to determine the flexural properties of dental materials. This ISO test standard requires a beam specimen of 25 x 2 x 2 mm for the three-point bending test.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Remineralizing Ability and Mechanical Properties of Two Fluoride Rechargeable Fissure Sealants in Primary Molars: An In vitro Study

Hussien,

Awad,

El Hosainy

2024

Egyptian Dental Journal

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Aim: This study was conducted to assess the remineralizing ability, microleakage and flexural strength of resin-based and bioactive-based sealants in primary teeth.Methods: Thirty-two exfoliated second primary molars were prepared and sealed with one of the following materials: BeautiSealant and Helioseal F plus. Sixteen samples were subjected to a pH-cycling model (7 days of demineralization-remineralization cycles). Scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX) were used to assess the remineralizing abilities before and after the studied sealants applied. The other (16) samples were subjected to a thermocycling model to assess the microleakage after sealants application. Flexural strength for each sealant material was also assessed following the ISO 4049 protocols using a Universal Testing Machine. Data were collected, tabulated then statistically analyzed using the Statistical Package of Social Science (SPSS) program Windows (Standard version 22).Results: After sealants application, the mean weight percentages of calcium (32.4,32.8), phosphate (15.7,16.6) and fluoride (13.6,14.02) showed a significant increase for Helioseal F and BeautiSealant respectively compared with baseline values. The mean Calcium/ Phosphate ratio (Ca/P) for Helioseal F plus was 2.163 and 1.796 for BeautiSealant after sealant application with no statistical significance difference was found. On the other hand, Helioseal F plus showed higher significance difference than BeautiSealant in flexural strength test. Conclusion:The bioactive giomer glass ionomer-based sealants could be used successfully as alternative to resin-based sealants as they showed comparable remineralizing ability and mechanical properties.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Remineralizing Ability and Mechanical Properties of Two Fluoride Rechargeable Fissure Sealants in Primary Molars: An In vitro Study

Hussien,

Awad,

El Hosainy

2024

Egyptian Dental Journal

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“…We used the methylene blue dye penetration test, which is not only an inexpensive and easily accomplished test but is also known to be a reliable method of testing microleakage at the sealant margins (Agrawal and Shigli 2012 ; Kramer et al 2008 ). While the method is associated with the concerns of acute methylene blue toxicity upon oral intake (Amend et al 2021 ), it should be noted that the present study was carried out in vitro.…”

Section: Discussionmentioning

confidence: 99%

A novel application of a bioactive material as a pit and fissure sealant: in vitro pilot study evaluating the sealing ability and penetration

Bishayi

Srinivasan

Karuna

et al. 2022

Eur Arch Paediatr Dent

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Purpose To compare the sealing ability and penetration of a bioactive material used as pit and fissure sealant to those of glass ionomer sealant. Methods This was an in vitro experimental study conducted on 20 permanent teeth. For Group I of ten teeth, ACTIVA BioACTIVE-Base/Liner was applied as a sealant on pits and fissures, and the remaining ten teeth of Group II were sealed using glass ionomer cement. After thermocycling, the apex of the teeth was sealed using composite resin and they were immersed in 1% methylene blue solution, buffered at pH 7 for 24 h. Longitudinal sections were obtained from each tooth for evaluating the sealing ability and penetration, using a binocular light microscope at 4 × magnification. The obtained data were subjected to analysis using the Chi-square test and independent t test. Results The comparison of the sealing ability and sealant penetration, between the two groups, showed statistically no significant difference (p = 0.104 and p = 1.0, respectively). Conclusion Bioactive material as a pit and fissure sealant, performed on par with glass ionomer sealant in terms of tested properties like sealing ability and penetration.

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“…-Both outcomes were assessed by supervisors of thesis twice with a 2-weeks interval to assess intra-examiner reliability while inter-examiner reliability was measured by comparing the scores of both assessors at the first assessment. (22) The inter-examiner and intra-examiner Cohen's kappa scores were 0.85 and 0.88 respectively.…”

Section: Outcome Assessmentmentioning

confidence: 90%

“…-After thermocycling, The specimens' apices were sealed with sticky wax (Hiflex, Prevest Direct, India). (22) -Each sample's surface was covered by a double layer of nail polish applied 1mm away of toothsealant conjunction. (23) -All of the samples were immersed in 2% methylene blue dye solution (SD Fine-Chem limited, Mumbai, India) for 24 hours at room temperature.…”

Section: E) Microleakage and Penetration Depth Assessmentmentioning

confidence: 99%

Effect of Laser Etching Versus Self-Etching Adhesive and Conventional Acid-Etching Techniques on Shear Bond Strength, Microleakage and Penetration Depth of a Resin Based Pit and Fissure Sealant (An In Vitro Study)

Ebrahim,

Wassel,

Hamdy

2023

Egyptian Dental Journal

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Objectives:The aim of the present study was to compare the effect of enamel pre-treatment with: 37% phosphoric acid-etching or self-etching adhesive and Er:YAG Laser (2940nm) on shear bond strength, microleakage and penetration depth of a fluoride-releasing resin-based pit and fissure sealant applied to sound premolars.Methods: Seventy-two sound premolars were allocated randomly into 3 groups according to enamel pretreatment protocol. The experimental groups included: Group I: 37% phosphoric acidetching, Group II: self-etching adhesive, Group III: Er:YAG Laser treatment. Each group was divided into two subgroups (A: shear bond strength, B: microleakage and penetration depth). Shear bond strength test was performed using Universal testing machine. Microleakage and penetration depth were tested after thermocycling (500×, 5-55°C, dwell time: 30s) and dye penetration method. Samples were assessed under Stereo-microscope with a magnification of ×40. One-way ANOVA followed by Tukey's post hoc test was used to analyze data.Results: Shear bond strength and microleakage results showed that there was statistically significant difference among different groups while penetration depth results showed there was no statistically significant difference among all groups.Conclusions: Conventional acid etching was the best pretreatment method prior to resin-based fissure sealants in terms of bond strength and microleakage.

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Microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self‐etching primers/adhesives

Cited by 8 publications

References 26 publications

Evaluation of Remineralizing Ability and Mechanical Properties of Two Fluoride Rechargeable Fissure Sealants in Primary Molars: An In vitro Study

Evaluation of Remineralizing Ability and Mechanical Properties of Two Fluoride Rechargeable Fissure Sealants in Primary Molars: An In vitro Study

A novel application of a bioactive material as a pit and fissure sealant: in vitro pilot study evaluating the sealing ability and penetration

Effect of Laser Etching Versus Self-Etching Adhesive and Conventional Acid-Etching Techniques on Shear Bond Strength, Microleakage and Penetration Depth of a Resin Based Pit and Fissure Sealant (An In Vitro Study)

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