Microinjection of mouse phospholipase Cζ complementary RNA into mare oocytes induces long-lasting intracellular calcium oscillations and embryonic development

Bedford-Guaus, Sylvia J.; Yoon, Sook‐Young; Fissore, Rafael A.; Choi, Young-Ho; Hinrichs, Katrin

doi:10.1071/rd08115

Cited by 29 publications

(26 citation statements)

References 48 publications

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“…We have shown previously that injection of stallion sperm extract induces calcium oscillations in both horse and mouse oocytes (Bedford et al, 2003). In an attempt to develop a defined method for induction of physiological [Ca 2þ ] i oscillations and thus activation, we evaluated the effect of injection of murine PLCz complementary (c)RNA on parthenogenetic activation of horse oocytes (Bedford-Guaus et al, 2008). Injection of murine PLCz cRNA alone induced longlasting calcium oscillations and parthenogenetic activation in equine oocytes, but only low rates of development to blastocyst (0 to 11%).…”

Section: Introductionmentioning

confidence: 99%

Effect of Sperm Extract Injection Volume, Injection of PLCζ cRNA, and Tissue Cell Line on Efficiency of Equine Nuclear Transfer

Choi

Hartman²,

Fissore

et al. 2009

Cloning and Stem Cells

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We evaluated the effect of different activation methods on blastocyst development after equine nuclear transfer. All activation treatments were followed by incubation in 2 mM 6-dimethylaminopurine for 4 h. In Experiment 1, reconstructed oocytes were injected with sperm extract for 0.1, 0.2, 0.4, 0.8, or 1.6 sec using a FemtoJet injection device, then treated with ionomycin. The blastocyst rate (9.8%) for 0.1-sec injection was significantly higher than that for 0.2 sec (0%) or 0.8 sec (1.4%). In Experiment 2, injection of murine PLCzeta cRNA before or after ionomycin treatment did not increase blastocyst development (0 and 4.5%) over a control treatment (injection of sperm extract after ionomycin exposure; 5.6%). Transfer of 10 blastocysts produced in Experiments 1 and 2 resulted in five pregnancies, all lost before 70 days of gestation. In Experiment 3, cells from a second biopsy sample from the same horse produced significantly more blastocysts than did the original sample (4/44 vs. 0/58; p < 0.05). Transfer of these four blastocysts produced two viable foals. In Experiment 4, blastocyst development rates did not differ between oocytes in metaphase I or II at the time of nuclear transfer (16.7 and 3.0%, respectively). A healthy foal was produced from a blastocyst originating from a metaphase I oocyte.

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Section: Introductionmentioning

confidence: 99%

Effect of Sperm Extract Injection Volume, Injection of PLCζ cRNA, and Tissue Cell Line on Efficiency of Equine Nuclear Transfer

Choi

Hartman²,

Fissore

et al. 2009

Cloning and Stem Cells

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“…Data in the latter study and in follow up reports provided strong evidence to support the concept that PLCz is the pivotal, and possibly exclusive, initiator of [Ca 2þ ] i oscillations in mammals. Specifically, injection of recombinant PLCz (Fujimoto et al 2004;Kouchi et al 2004) or PLCz cRNA evoked spermlike oscillations in mouse (Saunders et al 2002), rat (Ito et al 2008b), human (Rogers et al 2004), bovine (Malcuit et al 2005;Ross et al 2008), porcine (Yoneda et al 2006), and equine (Bedford-Guaus et al 2008) eggs. In vitro PLC assays, using recombinant PLCz confirmed the enzyme's high sensitivity to Ca 2þ , which render it nearly fully active at basal [Ca 2þ ] i concentrations ).…”

Section: Identification Of Plczmentioning

confidence: 99%

Ca2+ Signaling During Mammalian Fertilization: Requirements, Players, and Adaptations

Wakai¹,

Vanderheyden²,

Fissore³

2011

Cold Spring Harbor Perspectives in Biology

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Changes in the intracellular concentration of calcium ([Ca 2þ ] i ) represent a vital signaling mechanism enabling communication among cells and between cells and the environment. The initiation of embryo development depends on a [Ca 2þ ] i increase(s) in the egg, which is generally induced during fertilization. The [Ca 2þ ] i increase signals egg activation, which is the first stage in embryo development, and that consist of biochemical and structural changes that transform eggs into zygotes. The spatiotemporal patterns of [Ca 2þ ] i at fertilization show variability, most likely reflecting adaptations to fertilizing conditions and to the duration of embryonic cell cycles. In mammals, the focus of this review, the fertilization [Ca 2þ ] i signal displays unique properties in that it is initiated after gamete fusion by release of a sperm-derived factor and by periodic and extended [Ca 2þ ] i responses. Here, we will discuss the events of egg activation regulated by increases in [Ca 2þ ] i , the possible downstream targets that effect these egg activation events, and the property and identity of molecules both in sperm and eggs that underpin the initiation and persistence of the [Ca 2þ ] i responses in these species.A n increase in the intracellular concentration of calcium ([Ca 2þ ] i ) underlies the initiation, progression and/or completion of a wide variety of cellular processes, including fertilization, muscle contraction, secretion, cell division, and apoptosis (Berridge et al. 2000). To survive and proliferate, cells and organisms must communicate, and changes in [Ca 2þ ] i allow them to quickly respond to environmental, nutritional, or ligand challenges with responses that regulate cell fate and function. Cells devote significant amounts of their energy reserves to create and maintain ionic gradients between extracellular and intracellular milieus and also within the latter, thereby allowing brief alterations in these gradients to have profound signaling effects. In the case of Ca 2þ , myriad proteins have acquired the ability to bind Ca 2þ , which allows them to interpret and transform these elevations into cellular functions. This review will examine the cellular modifications induced by [Ca 2þ ] i changes during fertilization in mature mammalian oocytes, henceforth referred to as eggs.Oocytes during maturation ready themselves for fertilization and the initiation of embryogenesis. During this transition, oocytes undergo changes that include the resumption and progression of meiosis, the development of polyspermy-preventing mechanisms, the reorganization of the cytoskeleton with spindle formation and displacement to the cortex, and the translation, accumulation, and degradation of specific mRNAs and proteins involved in development (Horner and Wolfner 2008b (Stricker 1999;Miyazaki and Ito 2006). Elucidation of the signaling cascades and identification of the molecules/receptor(s) that initiate the Ca 2þ signal at fertilization has proven elusive, and this review will not dwell on th...

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“…The pattern of these [Ca 2þ ] i oscillations is species specific, with increases occurring at approximately 3-10-min intervals in the mouse [1,5], 10-35-min intervals in the human [6,7], and 20-50-min intervals in the bovine oocyte [1,3], and lasting for several hours. Studies in mare oocytes suggest that [Ca 2þ ] i oscillations might be similar in pattern and duration to those observed in the bovine species [4,8,9].…”

Section: Phospholipase C Zeta: a Protein Required For Fertilitymentioning

confidence: 79%

“…In this regard, the fidelity of the pattern and overall duration of [Ca 2þ ] i transients at fertilization is a crucial determinant of rates of preand postimplantation development [11]. For instance, we reported that although microinjection of murine PLCZ cRNA was able to support long-lasting [Ca 2þ ] i oscillations in mare oocytes, rates of development to the blastocyst stage were low (3%-15%) [9]. Similarly, others have reported a lesser efficiency in the ability of mouse PLCZ versus bovine PLCZ cRNA to support embryonic development of bovine oocytes [32].…”

Section: Sequence Specificity: Equine Plcz Yields a Protein With Highmentioning

confidence: 97%

“…Indirect evidence also supports a role for PLCZ in the fertilization-induced activation process in the horse. For instance, injection of sperm extracts, sperm (intracytoplasmic sperm injection [ICSI]), or murine PLCZ cRNA into mare oocytes triggered [Ca 2þ ] i oscillations and activation of the developmental program [4,8,9]. Moreover, stallion sperm and testis express PLCZ that was shown to be enzymatically active when injected into mouse oocytes [22].…”

Section: Phospholipase C Zeta: a Protein Required For Fertilitymentioning

confidence: 98%

See 1 more Smart Citation

Characterization of Equine Phospholipase C Zeta: A Review and Preliminary Results on Expression Defects in Subfertile Stallions

Bedford-Guaus

McPartlin

Varner

2012

Journal of Equine Veterinary Science

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Microinjection of mouse phospholipase Cζ complementary RNA into mare oocytes induces long-lasting intracellular calcium oscillations and embryonic development

Cited by 29 publications

References 48 publications

Effect of Sperm Extract Injection Volume, Injection of PLCζ cRNA, and Tissue Cell Line on Efficiency of Equine Nuclear Transfer

Effect of Sperm Extract Injection Volume, Injection of PLCζ cRNA, and Tissue Cell Line on Efficiency of Equine Nuclear Transfer

Ca2+ Signaling During Mammalian Fertilization: Requirements, Players, and Adaptations

Characterization of Equine Phospholipase C Zeta: A Review and Preliminary Results on Expression Defects in Subfertile Stallions

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