Microinjection of cis-flupenthixol, a dopamine antagonist, into the medial preoptic area impairs sexual behavior of male rats

Pehek, Elizabeth A.; Warner, Robert K.; Bazzett, Terence J.; Bitran, Daniel; Band, Linda C.; Eaton, Robert C.; Hull, Elaine M.

doi:10.1016/0006-8993(88)91599-5

Cited by 92 publications

(46 citation statements)

References 17 publications

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“…Stimuli from a receptive female rat and/or the act of copulation stimulate the release of DA in all three areas [26]. Microinjections of cisflupenthixol into the MPOA in rats decreased the percent of males copulating, the percent males ejaculating, and the frequencies of intromissions and ejaculations among males who did copulate [35,42], demonstrating the MPOA's importance in coordinating male sexual behavior.…”

Section: Discussionmentioning

confidence: 93%

“…Studies in Japanese quail [8,9,15] have demonstrated the importance of DA in coordinating sexual behavior in male birds as well. Administering cis-flupenthixol caused dramatic deficits in sexual motivation and performance in rats [10,26,42] and rabbits [1,2] without affecting motor performance.…”

Section: Discussionmentioning

confidence: 95%

“…Because we had previously found that 12 days of treatment with cis-flupenthixol during development caused striking reductions in courtship singing and sexual behavior when the males reached adulthood [21]. and because treatment with this drug reduced male sexual behavior in other species [1,35,42], we predicted that drug treatment woulddecrease courtship singing and copulatory behavior. These comparisons were done with one-tailed tests.…”

Section: Statistical Analysesmentioning

confidence: 99%

“…This drug has been widely used to investigate the involvement of DA in the control of male sexual behavior in mammals [1,35,42] …”

Section: Introductionmentioning

confidence: 99%

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Dopaminergic modulation of reproductive behavior and activity in male zebra finches

Rauceo

Harding

Maldonado

et al. 2008

Behavioural Brain Research

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We previously demonstrated that hormone treatments which stimulate female-directed singing increased levels and turnover of dopamine (DA) in brain areas controlling the motor patterning of song. To help determine how DA affects singing, we quantified the effects of treating adult male finches with the D 1 /D 2 receptor antagonist cis-flupenthixol. Adult males were given subcutaneous silastic implants of androgen, in case drug treatment interfered with androgen secretion. One week later, they were tested with females. Males were divided into three groups matched for levels of courtship singing. Males were then subcutaneously implanted with osmotic minipumps containing either saline, a low, or a high dose of cis-flupenthixol. Each male was tested with a different female 5 and 10 days after implantation to determine how this D 1 /D 2 receptor antagonist affected behavior. Both drug doses affected female-directed singing 5 days after initiation of treatment. High-dose males sang to females significantly less often than males in the other two groups. Low-dose males showed fewer high-intensity courtship displays in which males dance towards females as they sing. These effects on courtship singing were not seen at day 10, though other behavioral effects were seen at this time. Male beak wipes, rocks, following females and female withdrawals from males were also affected by drug treatment. General activity in the home cage was decreased by day 11. These data demonstrate that singing and several other female-directed behaviors are sensitive to perturbations in DA receptor function.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 95%

Section: Statistical Analysesmentioning

confidence: 99%

“…This drug has been widely used to investigate the involvement of DA in the control of male sexual behavior in mammals [1,35,42] …”

Section: Introductionmentioning

confidence: 99%

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Dopaminergic modulation of reproductive behavior and activity in male zebra finches

Rauceo

Harding

Maldonado

et al. 2008

Behavioural Brain Research

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“…Extensive data suggest that the release of DA into the preoptic area (POA) controls male sexual behavior. Administrations of the DA agonist apomorphine into the medial POA facilitate sexual behavior in male rats (Hull et al, 1986;Dominguez et al, 2001), whereas injections of the DA antagonist cis-flupenthixol into the medial POA reduce ejaculations, copulation rate, erections and penile movements before copulation, and sexual motivation (Pehek et al, 1988;Warner et al, 1991). Extracellular DA in male rats is increased in the medial POA during copulation and exposure to a receptive female (Hull et al, 1995), and in the paraventricular nucleus of the hypothalamus during copulation (Melis et al, 2003).…”

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confidence: 99%

Fos responses of dopamine neurons to sociosexual stimuli in male zebra finches

2006

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Dopamine (DA) is produced in numerous brain areas and influences a wide variety of social behaviors, but very few data are available to establish the socially-relevant response properties of most DA populations, which comprise eight cell groups numbered A8-A15. Anatomically, these DA populations are evolutionarily conserved, and all have been identified in both birds and mammals. We now report the Fos responses of tyrosine hydroxylase-immunoreactive (TH-ir; putatively dopaminergic) neurons in the A8-A15 cell groups of male zebra finches following exposure to a control condition or one of six different social stimuli: A heterospecific male, conspecific male, fighting in a mate competition paradigm (which includes both male and female stimuli), a courtship interaction without physical contact, a courtship interaction with physical contact but no mounting, and a courtship interaction with mounting. We found that the DA cell groups exhibit distinctive profiles of responsiveness to social stimuli. Fos induction in A8, A9, A10 and midbrain A11 neurons increased significantly in response to a variety of conspecific stimuli, but not heterospecific stimuli. In contrast, Fos induction in the preoptic A14 neurons was observed specifically in response to sexual interactions, and Fos induction in hypothalamic A11 neurons appears to primarily reflect the performance of courtship singing. Infundibular A12 neurons, which may be involved in stress-related processes, showed the highest level of TH+Fos colocalization in control subjects. This colocalization decreased in response to all conspecific stimuli except fighting, and did not decrease following exposure to a heterospecific male. Keywords dopamine; forebrain; aggression; sexual behavior; tyrosine hydroxylase; Fos Dopamine (DA) is known to influence a variety of social behaviors, particularly male sexual behavior, in multiple vertebrate groups (Warner et al., 1991;Pomerantz, 1992;Absil et al., 1994;Hull et al., 1995;Dominguez et al., 2001;Woolley et al., 2001;Melis et al., 2003;Charlier et al., 2005), and DA cells are distributed in homologous groups across the vertebrate classes (Bailhache and Balthazart, 1993;Bottjer, 1993;Gonzalez and Smeets, 1994;Reiner et al., 1994;Tillet, 1994;Appeltants et al., 2001;Adrio et al., 2002). This similarity across vertebrates is particularly clear when comparing mammals and birds, which each have dopaminergic neurons (immunopositive for tyrosine hydroxylase, TH, and immunonegative for dopamine β-hydroxylase) localized to eight populations of the basal forebrain and brainstem numbered A8 through A15 (Reiner et al., 1994;Tillet, 1994). However, it is largely unknown how these various DA populations respond to a broad range of social stimuli, so we have here examined DA neuronal responses to a variety of interactions with heterospecific and conspecific animals. Extensive data suggest that the release of DA into the preoptic area (POA) controls male sexual behavior. Administrations of the DA agonist apomorphine into the medial POA faci...

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Localization of Peripheral Dopamine D1 and D2 Receptors in Rat and Human Seminal Vesicles

Hyun

Baig

Yang

et al. 2002

Journal of Andrology

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Dopamine, an established neurotransmitter in the central nervous system, is recognized for its role in penile erection and ejaculation in rats. However, its complete mechanism of action in the genitourinary tract is unknown. The objective of this study was to investigate the existence and expression of peripheral dopamine D1 and D2 receptor messenger RNAs (mRNAs) and corresponding proteins in rat and human seminal vesicles. The seminal vesicle tissues of male Sprague-Dawley rats and human radical prostatectomy specimens were used to extract total RNA and proteins, and to prepare slide sections. Rat hypothalamus tissue served as a control for dopamine D1 and D2 receptors. Testing for the presence and expression of peripheral dopamine D1 and D2 receptor mRNAs in rat and human seminal vesicle tissues was performed by reverse transcription-polymerase chain reaction. Western blotting was used to detect corresponding proteins of D1 and D2 receptors. Immunohistochemical staining using rabbit antipeptide polyclonal antibodies was employed to identify and anatomically localize dopamine D1 and D2 receptor proteins in rat and human seminal vesicles. Dopamine D1 and D2 receptor transcripts were detected in both human and rat seminal vesicle tissues. Western blot analysis demonstrated that peripheral dopamine D1 and D2 receptor proteins exist in both human and rat seminal vesicle tissues. Immunohistochemical analysis demonstrated the localization of peripheral dopamine D1 and D2 receptors to the smooth muscle layer of human and rat seminal vesicles. The results of this study demonstrate that peripheral dopamine D1 and D2 receptors are present in the seminal vesicle tissue in both rats and humans. Although these results suggest that seminal emission may be mediated in part by the stimulation of peripheral dopamine receptors located in the seminal vesicles, the functional significance of dopamine in male reproductive tract has yet to be fully defined. E jaculation is governed by 3 interacting levels of the nervous system: the modulator influence, which originates from the supraspinal level; interaction at the spinal cord level; and sensory input, which determines the amplitude of the sacral reflex. These events occur reflexively, and require coordination of the autonomic and somatic nervous systems. The precise neurophysiological mechanism that controls these reflexes is not well understood. It has been suggested that the serotonergic and dopaminergic systems have both inhibitory and excitatory influences on penile erection and ejaculation at the supraspinal level (Hull et al, 1992;Olivier et al, 1998).Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure, and intracellular ion transport. The actions of dopamine on target tissues are mediated by cell surface receptors. Recently, atCorrespondence to: Wayne J.G. Hellstrom, MD, FACS, Professor of Urology, Tulane University Health Sciences Center, 1430 Tulane Ave, SL-42, New Orl...

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Microinjection of cis-flupenthixol, a dopamine antagonist, into the medial preoptic area impairs sexual behavior of male rats

Cited by 92 publications

References 17 publications

Dopaminergic modulation of reproductive behavior and activity in male zebra finches

Dopaminergic modulation of reproductive behavior and activity in male zebra finches

Fos responses of dopamine neurons to sociosexual stimuli in male zebra finches

Localization of Peripheral Dopamine D1 and D2 Receptors in Rat and Human Seminal Vesicles

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