Microinjection and transfer of cytoplasm in Paramecium

Koizumi, Sadaaki

doi:10.1016/0014-4827(74)90619-3

Cited by 94 publications

(12 citation statements)

References 9 publications

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“…The removal and transfer of macronuclei were carried out by micromanipulation (Koizumi, 1974). A cover glass with a drop containing the cell whose micronucleus was to be removed or implanted was placed over the moist chamber and manipulated using a phase microscope with a long working distance NIKON‐LWD condenser (Nikon, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Interactions between newly developed macronuclei and maternal macronuclei in sexually immature multinucleate exconjugants of Paramecium caudatum

Kimura

Mikami

2003

Differentiation

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Section: Methodsmentioning

confidence: 99%

Interactions between newly developed macronuclei and maternal macronuclei in sexually immature multinucleate exconjugants of Paramecium caudatum

Kimura

Mikami

2003

Differentiation

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“…Microinjection of cytoplasm (5-10 x 10' pin') was performed with a Chambers micromanipulator with a single needle afterthe method of Koizumi (12). Before microinjection the cells to be injected were placed in immobilization solution (Dryl's solution containing 0.5% (wt/vol) methylcellulose) .…”

Section: Methodsmentioning

confidence: 99%

Restoration of membrane excitability in a behavioral mutant of Paramecium caudatum during conjugation and by microinjection of wild-type cytoplasm.

Hiwatashi¹,

Haga²,

Takahashi³

1980

The Journal of Cell Biology

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When cells of the behavioral mutant cnrC of Paramecium caudatum were mated with the wild type, phenotype change from CNR (no backward swinning) to wild type in the cnrC mate occurred immediately after the formation of tight pairs. No change of phenotype occurred when cells of cnrA or cnrB were mated with wild type. Phenotypic change from CNR to wild type in cells of cnrC was also induced by microinjection of wild-type cytoplasm. Microinjection of wild-type cytoplasm induced no change in cells of cnrA or cnrB. Phenotypic change in the cnrC mate during conjugation can be explained by cytoplasmic exchange during conjugation, though transfer of membrane sites for excitability through membrane fluidity cannot be ruled out.

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“…It contains both micronuclear and macronuclear deletions of the A surface protein gene (13,15) as well as a complete macronuclear deletion of the B surface protein gene (17). It (9) and Mikami and Koizumi (12). Since P. tetraurelia contains two micronuclei, two steps of manipulation were necessary.…”

Section: Methodsmentioning

confidence: 99%

Non-Mendelian inheritance of macronuclear mutations is gene specific in Paramecium tetraurelia.

Scott¹,

Mikami²,

Leeck³

et al. 1994

Mol. Cell. Biol.

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The ciliated protozoan Paramecium tetraurelia possesses two kinds of nuclei within a single cell. The highly polyploid macronucleus is transcriptionally active and therefore determines the phenotype of the cell. In contrast, the two diploid micronuclei are transcriptionally silent but participate in the nuclear reorganization events of autogamy (self-fertilization) and conjugation (reciprocal exchange of nuclei with cell of the opposite mating type). During either of these events, a new macronuclear genome is produced from the micronuclear genome through a process which involves extensive amplification and rearrangement of micronuclear DNA. This process of macronuclear development results in differences in the genetic content of the two types of nuclei.The previously isolated mutant d48 provides an example of how the macronuclear content in the Paramecium cell can differ from the micronuclear content.

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Microinjection and transfer of cytoplasm in Paramecium

Cited by 94 publications

References 9 publications

Interactions between newly developed macronuclei and maternal macronuclei in sexually immature multinucleate exconjugants of Paramecium caudatum

Interactions between newly developed macronuclei and maternal macronuclei in sexually immature multinucleate exconjugants of Paramecium caudatum

Restoration of membrane excitability in a behavioral mutant of Paramecium caudatum during conjugation and by microinjection of wild-type cytoplasm.

Non-Mendelian inheritance of macronuclear mutations is gene specific in Paramecium tetraurelia.

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