Microinjection and Cell Transplantation in Zebrafish Embryos

Xu, Qiling; Stemple, Derek L.; Joubin, Katherine

doi:10.1007/978-1-60327-483-8_35

Cited by 16 publications

(9 citation statements)

References 19 publications

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“…Overexpression of genes can be achieved by simply injecting RNA into the embryo or by generating transgenic fish with the Tol2 vector system (Kawakami, 2007; Xu et al , 2008). Knockdown of a gene can be performed with antisense morpholino oligonucleotides that block either mRNA splicing or translation (Shestopalov and Chen, 2010).…”

Section: Discussionmentioning

confidence: 99%

New Insights into Signaling During Myelination in Zebrafish

Raphael

Talbot

2011

Current Topics in Developmental Biology

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Myelin is a vertebrate adaptation that allows for the rapid propagation of action potentials along axons. Specialized glial cells – oligodendrocytes in the central nervous system (CNS) and Schwann cells in the peripheral nervous system (PNS) – form myelin by repeatedly wrapping axon segments. Debilitating diseases result from the disruption of myelin, including Multiple Sclerosis and Charcot-Marie-Tooth peripheral neuropathies. The process of myelination involves extensive communication between glial cells and the associated neurons, and the last few years have seen important progress in understanding the molecular basis of the signals that coordinate the development of these fascinating cells. This review highlights recent advances in myelination deriving from studies in the zebrafish model system, with a primary focus on the PNS. While Neuregulin1-ErbB signaling has long been known to play important roles in peripheral myelin development, work in zebrafish has elucidated its roles in Schwann cell migration and radial sorting of axons sorting in vivo. Forward genetic screens in zebrafish have also uncovered new genes required for development of myelinated axons, including gpr126, which encodes a G-protein coupled receptor required for Schwann cells to progress beyond the promyelinating stage. In addition, work in zebrafish uncovered new roles for Schwann cells themselves, including in regulating the boundary between the PNS and CNS and positioning a nerve after its initial outgrowth.

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Section: Discussionmentioning

confidence: 99%

New Insights into Signaling During Myelination in Zebrafish

Raphael

Talbot

2011

Current Topics in Developmental Biology

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“…Transplants were performed essentially as described [63]. Briefly, 1–2-cell stage donor embryos were injected with lysine-fixable fluorescein or rhodamine labelled dextran (10,000 kDa, 1% in sterile water; Molecular Probes).…”

Section: Methodsmentioning

confidence: 99%

Jamb and Jamc Are Essential for Vertebrate Myocyte Fusion

Powell

Wright

2011

PLoS Biol

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“…Cell and tissue transplantation techniques have been used in zebrafish embryos to investigate the state of cell commitment, inductive interactions between embryonic tissues, and defective tissues in mutant embryos (100,101). More recently, the zebrafish system has emerged as reliable model for tumor transplantation assays (102,103).…”

Section: Transplantation Of Tissuesmentioning

confidence: 99%

Hooked! Modeling human disease in zebrafish

Santoriello¹,

Zon²

2012

J. Clin. Invest.

439

326

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Zebrafish have been widely used as a model system for studying developmental processes, but in the last decade, they have also emerged as a valuable system for modeling human disease. The development and function of zebrafish organs are strikingly similar to those of humans, and the ease of creating mutant or transgenic fish has facilitated the generation of disease models. Here, we highlight the use of zebrafish for defining disease pathways and for discovering new therapies.Conflict of interest: Leonard I. Zon is a founder and stockholder of Fate Inc. and a scientific advisor for Stemgent. Citation for this article:

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Microinjection and Cell Transplantation in Zebrafish Embryos

Cited by 16 publications

References 19 publications

New Insights into Signaling During Myelination in Zebrafish

New Insights into Signaling During Myelination in Zebrafish

Jamb and Jamc Are Essential for Vertebrate Myocyte Fusion

Hooked! Modeling human disease in zebrafish

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