Microgrooved-surface topography enhances cellular division and proliferation of mouse bone marrow-derived mesenchymal stem cells

Chaudhary, J. K.; Rath, Pramod C.

doi:10.1371/journal.pone.0182128

Cited by 32 publications

(18 citation statements)

References 42 publications

(62 reference statements)

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“…In the next step, we performed fluorescence-activated cell sorting (FACS) for the cell cycle analysis of BMCs treated with aspartame fibrils at 24 h (see Materials and Methods). We used a propidium iodide (PI) staining assay, which is most commonly used for DNA content/cell cycle analysis, 51 and such staining assay detects the differentiation of cells in sub-G1, G1, S, and G2/M phase, as well as identification of aneuploid populations (Figure S18, panel b). The results, as shown in panel c of Figure S18, clearly show suppression of G1 phase in cells treated with aspartame fibrils, indicating about ∼40% reduction in the presence of 10 mM aspartame aggregates.…”

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confidence: 99%

Self-Assembly of Artificial Sweetener Aspartame Yields Amyloid-like Cytotoxic Nanostructures

et al. 2019

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Recent reports have revealed the intrinsic propensity of single aromatic metabolites to undergo self-assembly and form nanostructures of amyloid nature. Hence, identifying whether aspartame, a universally consumed artificial sweetener, is inherently aggregation prone becomes an important area of investigation. Although the reports on aspartame-linked side effects describe a multitude of metabolic disorders, the mechanistic understanding of such destructive effects is largely mysterious. Since aromaticity, an aggregation-promoting factor, is intrinsic to aspartame’s chemistry, it is important to know whether aspartame can undergo self-association and if such a property can predispose any cytotoxicity to biological systems. Our study finds that aspartame molecules, under mimicked physiological conditions, undergo a spontaneous self-assembly process yielding regular β-sheet-like cytotoxic nanofibrils of amyloid nature. The resultant aspartame fibrils were found to trigger amyloid cross-seeding and become a toxic aggregation trap for globular proteins, Aβ peptides, and aromatic metabolites that convert native structures to β-sheet-like fibrils. Aspartame fibrils were also found to induce hemolysis, causing DNA damage resulting in both apoptosis and necrosis-mediated cell death. Specific spatial arrangement between aspartame molecules is predicted to form a regular amyloid-like architecture with a sticky exterior that is capable of promoting viable H-bonds, electrostatic interactions, and hydrophobic contacts with biomolecules, leading to the onset of protein aggregation and cell death. Results reveal that the aspartame molecule is inherently amyloidogenic, and the self-assembly of aspartame becomes a toxic trap for proteins and cells, exposing the bitter side of such a ubiquitously used artificial sweetener.

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confidence: 99%

Self-Assembly of Artificial Sweetener Aspartame Yields Amyloid-like Cytotoxic Nanostructures

et al. 2019

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“…Also, these changes have a measurable effect on cell growth, morphology, and phenotype/secretome. Previous studies demonstrated that microgrooved patterns favor stem-like phenotype [ 59 ]. Thus, substrates that favor cell elongation could enhance or retain the pluripotent capacity of the cells.…”

Section: Discussionmentioning

confidence: 99%

Polystyrene Topography Sticker Array for Cell-Based Assays

Rosado-Galindo

Domenech

2020

rpm

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Cells can respond to different topographical cues in their natural microenvironment. Hence, scientists have employed microfabrication techniques and materials to generate culture substrates containing topographies for cell-based assays. However, one of the limitations of custom topographical platforms is the lack of adoption by the broad research community. These techniques and materials have high costs, require high technical expertise, and can leach components that may introduce artifacts. In this study, we developed an array of culture surfaces on polystyrene using razor printing and sanding methods to examine the impact of microscale topographies on cell behavior. The proposed technology consists of culture substrates of defined roughness, depth, and curvature on polystyrene films bound to the bottom of a culture well using double-sided medical-grade tape. Human monocytes and adult mesenchymal stem cells (hMSCs) were used as test beds to demonstrate the applicability of the array for cell-based assays. An increase in cell elongation and Arg-1 expression was detected in macrophages cultured in grooves and on rough substrates as compared to flat surfaces. Also, substrates with enhanced roughness stimulated the proliferation of hMSCs. This effect correlated with the secretion of proteins involved in cell proliferation and the downregulation of those associated with cell differentiation. Our results showed that the polystyrene topography sticker array supports cellular changes guided by microscale surface roughness and geometries. Consequently, microscale surface topographies on polished and razor-printed polystyrene films could leverage the endogenous mechanisms of cells to stimulate cellular changes at the functional level for cell-based assays.

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“…For instance, human BM-stromal cells (hBM-SCs) cultured on a rough surface [arithmetic average roughness (Ra) 11.30 ± 0.43 µm] are more prone to differentiating into osteocytes than those on a smooth surface (Ra 0.05 ± 0.01 µm), with higher secretion of osteogenic-related protein Laminin-5 (Ln-5) and stronger activation of Ln-5 binding integrins [65]. On a microgrooved bearing surface partially mimicking the physiological reticulated microenvironment, mouse BM-derived MSCs showed a twofold to threefold increase in cell proliferation and expressed higher levels of pluripotency-related markers versus a standard 2D culture [66]. Within a certain diameter range (74-148 nm), the ability of TiO2 nanotubes to promote the osteogenic differentiation of MSCs strengthened with the increase of nanosize [67].…”

Section: Niche Geometrymentioning

confidence: 99%

Biomechanical cues as master regulators of hematopoietic stem cell fate

Luo

Shan

et al. 2021

Cell. Mol. Life Sci.

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Hematopoietic stem cells (HSCs) perceive both soluble signals and biomechanical inputs from their microenvironment and cells themselves. Emerging as critical regulators of the blood program, biomechanical cues such as extracellular matrix stiffness, fluid mechanical stress, confined adhesiveness, and cell-intrinsic forces modulate multiple capacities of HSCs through mechanotransduction. In recent years, research has furthered the scientific community’s perception of mechano-based signaling networks in the regulation of several cellular processes. However, the underlying molecular details of the biomechanical regulatory paradigm in HSCs remain poorly elucidated and researchers are still lacking in the ability to produce bona fide HSCs ex vivo for clinical use. This review presents an overview of the mechanical control of both embryonic and adult HSCs, discusses some recent insights into the mechanisms of mechanosensing and mechanotransduction, and highlights the application of mechanical cues aiming at HSC expansion or differentiation.

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Microgrooved-surface topography enhances cellular division and proliferation of mouse bone marrow-derived mesenchymal stem cells

Cited by 32 publications

References 42 publications

Self-Assembly of Artificial Sweetener Aspartame Yields Amyloid-like Cytotoxic Nanostructures

Self-Assembly of Artificial Sweetener Aspartame Yields Amyloid-like Cytotoxic Nanostructures

Polystyrene Topography Sticker Array for Cell-Based Assays

Biomechanical cues as master regulators of hematopoietic stem cell fate

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