Microgripper Using Soft Microactuators for Manipulation of Living Cells

Kodera, Shunnosuke; Watanabe, Tomoki; Yokoyama, Yoshihiko; Hayakawa, Takeshi

doi:10.3390/mi13050794

Cited by 10 publications

(5 citation statements)

References 32 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 106,107 ] g) Schematic of the microgripper using thermoresponsive gel actuators. [ 108 ] h) The particle is driven by optical tweezer to produce microvortexes, the intermediate particle represents cell. [ 109 ] Reproduced (Adapted) with permission.…”

Section: Single‐cell Pose Adjustment Methodsmentioning

confidence: 99%

“…Soft materials can also be used for single‐cell manipulation. Kodera et al [ 108 ] proposed a microgripper actuated by soft thermoresponsive hydrogels (Figure 4g). The gripper is driven by soft actuators using local heating from laser irradiation to manipulate live cells, which does not require complex control system.…”

Section: Single‐cell Pose Adjustment Methodsmentioning

confidence: 99%

See 1 more Smart Citation

A Review of Single‐Cell Pose Adjustment and Puncture

Guo

Zhang

Jin

et al. 2022

Advanced Intelligent Systems

View full text Add to dashboard Cite

Single‐cell manipulation technology is an essential method of cell research, with wide applications in biological engineering, medical engineering, agricultural engineering, and other precise manipulation fields. Cell pose adjustment and cell puncture are considered as two basic operations of single‐cell manipulation. Cell pose adjustment can be used for cell sorting, cell trapping, cell orientation, cell transportation, etc. It consists of direct contact manipulation methods and noncontact manipulation methods (e.g., mechanical contact method, electric field, optical field, magnetic field, acoustic field, and hydrodynamic methods). Cell puncture consists of ordinary glass needle puncture methods, piezoelectric‐assisted puncture methods, and rotational oscillatory drill puncture methods. It aims to achieve directional and quantified injection or sampling with minimal damage. Herein, the recent research progress on single‐cell pose adjustment and puncture methods is systematically summarized and discussed, which involves the basic mechanism, characteristics, limitations, and applications. Some potential directions for future research are proposed in accordance with the above analysis. It is hoped that this review can provide a reference for academic research and industrial applications of single‐cell manipulation technology.

show abstract

Section: Single‐cell Pose Adjustment Methodsmentioning

confidence: 99%

Section: Single‐cell Pose Adjustment Methodsmentioning

confidence: 99%

A Review of Single‐Cell Pose Adjustment and Puncture

Guo

Zhang

Jin

et al. 2022

Advanced Intelligent Systems

View full text Add to dashboard Cite

show abstract

“…These efforts entail the design of a structural configuration integrating an electrothermal actuator, magnetic and capacitive force sensors [20,21], or employing piezoelectric (PZT) actuators [22,23] to facilitate feedback on applied force. However, the reliance on sensors for the control of gripping force introduces complexity and instability into the system [24].…”

Section: Introductionmentioning

confidence: 99%

Design and fabrication of a multi-purpose four arm electrothermal microgripper; a novel approach to control maximum gripping force limit

Barati,

Barazandeh,

Jabari

et al. 2024

Phys. Scr.

View full text Add to dashboard Cite

The development of microgrippers requires accurate control of force and position for the grasping mechanisms in micromanipulation. This controllability ensures the safe transfer of sensitive micro-objects, such as living cells. The design of microgrippers is often limited by the dimensions and adaptability of the micro-objects being manipulated. Therefore, microgrippers should be customized for each micro-object. Additionally, complex sensor mechanisms are required to control the gripping force. This study introduces a novel design of a four-arm MEMS electrothermal microgripper intended for versatile micromanipulation purposes. The microgripper design procedure is compatible with a list of constraints, including biological and microfabrication constraints. The jaw form is designed to manipulate micro-objects with a wide range of dimensions (1 to 360 μm). Furthermore, a novel approach is presented here to control the force threshold of gripping without a sensor during manipulation. The proposed solution involves using structure stiffening to control the force and prevent damage to micro-objects. GA and analytical models (transient behaviour of structure) are used to satisfy the long list of constraints. The device is fabricated through UV-LIGA, utilizing nickel and copper as the structural and sacrificial layer. The experimental and simulation results demonstrate that the microgripper can achieve a 60 μm jaw displacement at a voltage of 0.329V. The gripping arms can provide a force of 15 to 450 μN for the handling of micro-objects. The maximum gripper temperature of 98 °C makes it suitable for biological applications. The innovative form and systematic design of the microgripper enable its adaptability for various applications.

show abstract

“…Compliant mechanisms have the advantages of high precision, no friction, no need for lubrication, and cost reduction. So, they are widely used in optical fiber alignment equipment, 1 medical instruments, 2,3 micro/nano‐manipulation 4,5 and micro‐electro mechanical systems (MEMS) 6 . According to the distribution pattern of compliance in the mechanisms, they can be divided into three types: lumped compliant mechanisms, distributed compliant mechanisms, and hybrid compliant mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Theoretical, numerical, and experimental investigation on the compliance and natural frequency of sinusoidal flexure hinges

Wang

Long

Wei

et al. 2023

Engineering Reports

View full text Add to dashboard Cite

To design a flexure hinge with high precision and high natural frequency, the sinusoidal flexure hinge is proposed in this article. First, the formulae for the compliance and precision factors of the hinge were derived based on the Euler–Bernoulli beam theory and the Gauss–Legendre quadrature formula. The natural frequency was also investigated based on the transfer matrix method. Compared with the simulation results of ANSYS Workbench, the results show that the modeling error is less than 6.7%. Second, the influence of structural parameters on compliance, precision factor, compliance precision ratio, and natural frequency was analyzed. The results show that compliance and precision are often contradictory, and the minimum thickness significantly influences the hinge's performance. Compared with conic flexure hinges in terms of compliance, precision, compliance precision ratios, and natural frequency, the sinusoidal flexure hinges have a better comprehensive performance. Finally, a flexure hinge was manufactured, and compliance was measured. The experimental results show that the error between the experimental value and the modeling value is 7.8%. Both simulation and experimental results verify the effectiveness of the sinusoidal flexure hinge model.

show abstract

Microgripper Using Soft Microactuators for Manipulation of Living Cells

Cited by 10 publications

References 32 publications

A Review of Single‐Cell Pose Adjustment and Puncture

A Review of Single‐Cell Pose Adjustment and Puncture

Design and fabrication of a multi-purpose four arm electrothermal microgripper; a novel approach to control maximum gripping force limit

Theoretical, numerical, and experimental investigation on the compliance and natural frequency of sinusoidal flexure hinges

Contact Info

Product

Resources

About