Microgravity-Induced Alterations of Inflammation-Related Mechanotransduction in Endothelial Cells on Board SJ-10 Satellite

Li, Ning; Wang, Chengzhi; Sun, Shan; Zhang, Chen; Lü, Dongyuan; Chen, Qin; Long, Mian

doi:10.3389/fphys.2018.01025

Cited by 42 publications

(41 citation statements)

References 79 publications

(127 reference statements)

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“…Aside from this, changes in gravity affect the organization of cytoskeletal proteins which orchestrate adaptation processes to mechanical stress 16–18 . Thus, permanent remodelling of the cytoskeleton by alternating µg and hyper-g conditions has also an effect on cell mechanical stability 19,20 , motility and adhesion 21 and cell signalling 11 .…”

Section: Introductionmentioning

confidence: 99%

Cells´ Flow and Immune Cell Priming under alternating g-forces in Parabolic Flight

Moser¹,

Sun

et al. 2019

Sci Rep

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Gravitational stress in general and microgravity (µg) in particular are regarded as major stress factors responsible for immune system dysfunction in space. To assess the effects of alternating µg and hypergravity (hyper-g) on immune cells, the attachment of peripheral blood mononuclear cells (PBMCs) to adhesion molecules under flow conditions and the antigen-induced immune activation in whole blood were investigated in parabolic flight (PF). In contrast to hyper-g (1.8 g) and control conditions (1 g), flow and rolling speed of PBMCs were moderately accelerated during µg-periods which were accompanied by a clear reduction in rolling rate. Whole blood analyses revealed a “primed” state of monocytes after PF with potentiated antigen-induced pro-inflammatory cytokine responses. At the same time, concentrations of anti-inflammatory cytokines were increased and monocytes displayed a surface molecule pattern that indicated immunosuppression. The results suggest an immunologic counterbalance to avoid disproportionate immune responses. Understanding the interrelation of immune system impairing and enhancing effects under different gravitational conditions may support the design of countermeasures to mitigate immune deficiencies in space.

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Section: Introductionmentioning

confidence: 99%

Cells´ Flow and Immune Cell Priming under alternating g-forces in Parabolic Flight

Moser¹,

Sun

et al. 2019

Sci Rep

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“…The reasons for not replacing cellular simulators instead of real flights are as the following [23]:…”

Section: Computer Simulation (Especially In Musculoskeletal Biomechanmentioning

confidence: 99%

Vitamin D in Space

Khoshvaghti¹

2019

Fads and Facts About Vitamin D

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Mankind has explored space since many years ago for distinct purposes. The space environment has its special features including microgravity (weightlessness), radiation, vacuum, and extreme temperature. It is fascinating to hear space traveling or even living on another planet, but it may cause dramatic changes in the human body. The skeleton has the primary role for the human body on the Earth and also in space. Osteoporosis is the principal feature in spaceflights occurring sooner or later. It will pose health risks. The aging of the population is the cause for osteoporosis to be more prevalent in the future. It seems that aging will happen sooner in space for human beings that are accustomed to living on the Earth. A complex of all reported changes that would occur in space, the picture of a closely resembling aged man emerges. Vitamin D can be synthesized and acts as a hormone. Its receptors are evident in more than 30 human tissues. Vitamin D has positive effects on the skeleton and other systems of the body in many regards. Multiple actions have been claimed for vitamin D (real or false), many aspects of which are not fully understood. The issue applies more about the vitamin and space, which has been tried to describe in this chapter.

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“…This device was mounted in the SJ-10 satellite launched on April 6 and recovered on April 18, 2016 (Supplemental Fig. S1C) (23,24). At 36 h before launch, 2 cell culture chambers with an effective culture area of 12 cm 2 each were filled with a cell suspension containing 5 3 10 5 rBMSCs per chamber and placed in an incubator for 12 h at 37°C to allow cell attachment.…”

Section: Cell Culture Hardware and Spaceflight Missionmentioning

confidence: 99%

“…Each substrate was then cut into pieces and used for scanning electron microscope (SEM) imaging or immunofluorescence (IF) staining. The SEM imaging was conducted as previously described (24). In short, 1 aliquot of the samples was dehydrated with ethanol from 50-70-80-90-100% for 5 min at each gradient, transferred to tert-butyl alcohol twice, dried at the critical point, coated with a thin layer of gold, and finally imaged by an FEI Quanta 200 SEM (Thermo Fisher Scientific).…”

Section: Optical and Scanning Electron Microscope Imagingmentioning

confidence: 99%

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Microgravity‐induced hepatogenic differentiation of rBMSCs on board the SJ‐10 satellite

et al. 2018

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Bone marrow‐derived mesenchymal stem cells (BMSCs) are able to differentiate into functional hepatocytelike cells, which are expected to serve as a potential cell source in regenerative medicine, tissue engineering, and clinical treatment of liver injury. Little is known about whether and how space microgravity is able to direct the hepatogenic differentiation of BMSCs in the actual space microenvironment. In this study, we examined the effects of space microgravity on BMSC hepatogenic differentiation on board the SJ‐10 Recoverable Scientific Satellite. Rat BMSCs were cultured and induced in hepatogenic induction medium for 3 and 10 d in custom‐made space cell culture hardware. Cell growth was monitored periodically in orbit, and the fixed cells and collected supernatants were retrieved back to the Earth for further analyses. Data indicated that space microgravity improves the differentiating capability of the cells by up‐regulating hepatocyte‐specific albumin and cytokeratin 18. The resulting cells tended to be maturated, with an in‐orbit period of up to 10 d. In space, mechanosensitive molecules of β1‐integrin, β‐actin, α‐tubulin, and Ras homolog gene family member A presented enhanced expression, whereas those of cell‐surface glycoprotein CD44, intercellular adhesion molecule 1, vascular cell adhesion molecule 1, vinculin, cell division control protein 42 homolog, and Rho‐associated coiled‐coil kinase yielded reduced expression. Also observed in space were the depolymerization of actin filaments and the accumulation of microtubules and vimentin through the altered expression and location of focal adhesion complexes, Rho guanosine 5′‐triphosphatases, as well as the enhanced exosome‐mediated mRNA transfer. This work furthers the understanding of the underlying mechanisms of space microgravity in directing hepatogenic differentiation of BMSCs.—Lii, D., Sun, S., Zhang, F., Luo, C., Zheng, L., Wu, Y., Li, N., Zhang, C., Wang, C., Chen, Q., Long, M. Microgravity‐induced hepatogenic differentiation of rBMSCs on board the SJ‐10 satellite. FASEB J. 33, 4273–4286 (2019). http://www.fasebj.org

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Microgravity-Induced Alterations of Inflammation-Related Mechanotransduction in Endothelial Cells on Board SJ-10 Satellite

Cited by 42 publications

References 79 publications

Cells´ Flow and Immune Cell Priming under alternating g-forces in Parabolic Flight

Cells´ Flow and Immune Cell Priming under alternating g-forces in Parabolic Flight

Vitamin D in Space

Microgravity‐induced hepatogenic differentiation of rBMSCs on board the SJ‐10 satellite

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