Microgravity-driven remodeling of the proteome reveals insights into molecular mechanisms and signal networks involved in response to the space flight environment

Rea, Giuseppina; Cristofaro, Francesco; Pani, Giuseppe; Pascucci, Barbara; Ghuge, Sandip A.; Corsetto, Paola Antonia; Imbriani, Marcello; Visai, Livia; Rizzo, Angela Maria

doi:10.1016/j.jprot.2015.11.005

Cited by 44 publications

(30 citation statements)

References 173 publications

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“…Kraft et al, 2000;Pardo et al, 2005;Patel et al, 2007), plants (Kittang et al, 2013) and invertebrates (Ricci and Boschetti, 2003), but to our knowledge this is the first successful ground-based simulation of microgravity for an adult vertebrate. Amphibious fishes such as K. marmoratus may be a useful experimental model for future experiments investigating weight-induced musculoskeletal remodelling (Rea et al, 2016;Reilly and Franklin, 2016). Adjustments to the morphology or mechanical properties of bones have widely been thought to require larger forces or strain rates than those imposed by gravitational loading (e.g.…”

Section: Weight Responsiveness In An Amphibious Fishmentioning

confidence: 99%

Skeletal stiffening in an amphibious fish out of water is a response to increased body weight

Turko

Kültz

Fudge

et al. 2017

Journal of Experimental Biology

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Terrestrial animals must support their bodies against gravity, while aquatic animals are effectively weightless because of buoyant support from water. Given this evolutionary history of minimal gravitational loading of fishes in water, it has been hypothesized that weight-responsive musculoskeletal systems evolved during the tetrapod invasion of land and are thus absent in fishes. Amphibious fishes, however, experience increased effective weight when out of water - are these fishes responsive to gravitational loading? Contrary to the tetrapod-origin hypothesis, we found that terrestrial acclimation reversibly increased gill arch stiffness (∼60% increase) in the amphibious fish when loaded normally by gravity, but not under simulated microgravity. Quantitative proteomics analysis revealed that this change in mechanical properties occurred via increased abundance of proteins responsible for bone mineralization in other fishes as well as in tetrapods. Type X collagen, associated with endochondral bone growth, increased in abundance almost ninefold after terrestrial acclimation. Collagen isoforms known to promote extracellular matrix cross-linking and cause tissue stiffening, such as types IX and XII collagen, also increased in abundance. Finally, more densely packed collagen fibrils in both gill arches and filaments were observed microscopically in terrestrially acclimated fish. Our results demonstrate that the mechanical properties of the fish musculoskeletal system can be fine-tuned in response to changes in effective body weight using biochemical pathways similar to those in mammals, suggesting that weight sensing is an ancestral vertebrate trait rather than a tetrapod innovation.

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Section: Weight Responsiveness In An Amphibious Fishmentioning

confidence: 99%

Skeletal stiffening in an amphibious fish out of water is a response to increased body weight

Turko

Kültz

Fudge

et al. 2017

Journal of Experimental Biology

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“…There are reports about various health concerns such as dysfunctions of the musculoskeletal and cardiovascular system, a down-regulation of the immune system, and visual problems [1-3]. In space, the thyroid gland in vivo revealed a variety of changes such as follicles with larger thyrocytes and increases in cAMP, thyrotropin-receptors (TSHR), and caveolin-1 [4, 5].…”

Section: Introductionmentioning

confidence: 99%

Cytokine Release and Focal Adhesion Proteins in Normal Thyroid Cells Cultured on the Random Positioning Machine

Warnke

Pietsch

Kopp

et al. 2017

Cell Physiol Biochem

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Background/Aims: Spaceflight impacts on the function of the thyroid gland in vivo. In vitro normal and malignant thyrocytes assemble in part to multicellular spheroids (MCS) after exposure to the random positioning machine (RPM), while a number of cells remain adherent (AD). We aim to elucidate possible differences between AD and MCS cells compared to 1g-controls of normal human thyroid cells. Methods: Cells of the human follicular epithelial thyroid cell line Nthy-ori 3-1 were incubated for up to 72 h on the RPM. Afterwards, they were investigated by phase-contrast microscopy, quantitative real-time PCR and by determination of cytokines released in their supernatants. Results: A significant up-regulation of IL6, IL8 and CCL2 gene expression was found after a 4h RPM-exposure, when the whole population was still growing adherently. MCS and AD cells were detected after 24 h on the RPM. At this time, a significantly reduced gene expression in MCS compared to 1g-controls was visible for IL6, IL8, FN1, ITGB1, LAMA1, CCL2, and TLN1. After a 72 h RPM-exposure, IL-6, IL-8, and TIMP-1 secretion rates were increased significantly. Conclusion: Normal thyrocytes form MCS within 24 h. Cytokines seem to be involved in the initiation of MCS formation via focal adhesion proteins.

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“…然而, NASA对执行过近地轨道飞行任务航天员健康状况的统计表明, 其寿命相对于美国人的平均寿命有所延长, 癌症和循环系统疾病的死亡率有所降低, 但白内障的发生率却明显增加 [16] . 总的来说, MSL的实际测量数据 [17,18] 和相应的健康风险预测结果 [8,23] . 然而, 目前关于空间辐射和微重力对航天员健康风险的影响是否存在协同效应尚不明确 [24,25] , 需要在后续的空间辐射风险评估、预警以及防护研究中加以考虑.…”

Section: 的火星科学实验室(Marsunclassified

Research progress of space radiation protection technologies in manned deep space exploration missions

Zhao¹,

Shang²,

Yuan³

et al. 2019

Chin. Sci. Bull.

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Microgravity-driven remodeling of the proteome reveals insights into molecular mechanisms and signal networks involved in response to the space flight environment

Cited by 44 publications

References 173 publications

Skeletal stiffening in an amphibious fish out of water is a response to increased body weight

Skeletal stiffening in an amphibious fish out of water is a response to increased body weight

Cytokine Release and Focal Adhesion Proteins in Normal Thyroid Cells Cultured on the Random Positioning Machine

Research progress of space radiation protection technologies in manned deep space exploration missions

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