Microglial Response to Gold Nanoparticles

Hutter, Eliza; Boridy, Sebastien; Labrecque, Simon; Lalancette–Hébert, Mélanie; Križ, Jasna; Winnik, Françoise M.; Maysinger, Dušica

doi:10.1021/nn901869f

Cited by 266 publications

(243 citation statements)

References 62 publications

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“…Also at doses from 0.2 mg/mL, for hexagonal GNPs, the Factin cytoskeleton was found deformed, caspases activity (indicates the strong activation of both intrinsic and extrinsic apoptotic pathways) increased and the expression of lysosomal enzyme Cathepsin-B heightened. The onset of apoptotic cell death within Calu-3 epithelial cells over the 72 hour exposure period to hexagonal GNPs can further be associated with the significant increase in non-mitochondrial ROS production since the induction of cell death can be elucidated by an increase in ROS, and are in support of the findings of a recent study by Hutter et al [6].…”

Section: Discussionsupporting

confidence: 85%

“…Due to their plasmonic properties, gold nanoparticles (GNPs) have been proposed as advantageous nano-sized materials for use in the various diagnostic and therapeutic applications, such as cell imaging, targeted drug delivery, thermal ablation, phototherapy [1][2][3][4][5][6][7].. To date spherical GNPs have been proven as one of the most biocompatible NP models for biological-based applications [8][9][10][11][12]. Recently, it has been reported that the specific shape of GNPs can be exploited to advantageously tune these nanomaterials for any biomedical application of choice [8,13].…”

Section: Introductionmentioning

confidence: 99%

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Investigating the Role of Shape on the Biological Impact of Gold Nanoparticles in Vitro

Tian

Clift

Casey³

et al. 2015

Nanomedicine (Lond.)

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Aim: To investigate the influence of gold nanoparticle (GNP) geometry on the biochemical response of Calu-3 epithelial cells. Materials and Methods:Spherical, triangular and hexagonal GNPs were used. The GNP-cell interaction was assessed via atomic absorption spectroscopy (AAS) and transmission electron microscopy (TEM). The biochemical impact of GNPs was determined over 72hrs at [0.0001-1mg/mL].Results: At 1mg/mL, hexagonal GNPs reduced Calu-3 viability below 60%, showed increased reactive oxygen species production and higher expression of pro-apoptotic markers. A cell mass burden of 1:2:12 as well as number of GNPs per cell (2:1:3) was observed for spherical:triangular:hexagonal GNPs. Conclusion:These findings do not suggest a direct shape-toxicity effect. However, do highlight the contribution of shape towards the GNP-cell interaction which impacts upon their intracellular number, mass and volume dose.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Investigating the Role of Shape on the Biological Impact of Gold Nanoparticles in Vitro

Tian

Clift

Casey³

et al. 2015

Nanomedicine (Lond.)

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“…This is different from other metal-based nanoparticles, including zinc and titanium oxide nanoparticles that have substantial toxicity in neurons and astrocytes. [26][27][28] Compared to these other metal nanoparticles, the IONP-based compositions used in the present study appear to have good biocompatibility with concentrations required to produce toxicity being rather large and not likely to be obtained in vivo or in clinical applications. 11,29 We had hypothesized that the extent of nanoparticle accumulation in cells would have important implications in the extent of potential toxicity produced.…”

Section: Discussionmentioning

confidence: 96%

“…The bEnd.3 cells (passage number [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] were cultured in Dulbecco's Modified Eagle Medium (DMEM) (Hyclone, Logan, UT) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Hyclone), 50 U/ mL penicillin and streptomycin (MP Biomedicals, Solon, OH, USA) at 37°C and 5% CO 2 . Cells were expanded in T-75 tissue culture flasks and seeded at 2 × 10 4 cells per cm 2 on six-or twelve-well plates for uptake and cytotoxicity studies, respectively.…”

Section: Cell Culturementioning

confidence: 99%

Characterization of cellular uptake and toxicity of aminosilane-coated iron oxide nanoparticles with different charges in central nervous system-relevant cell culture models

Sun¹,

Yathindranath²,

Worden³

et al. 2013

IJN

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Background: Aminosilane-coated iron oxide nanoparticles (AmS-IONPs) have been widely used in constructing complex and multifunctional drug delivery systems. However, the biocompatibility and uptake characteristics of AmS-IONPs in central nervous system (CNS)-relevant cells are unknown. The purpose of this study was to determine the effect of surface charge and magnetic field on toxicity and uptake of AmS-IONPs in CNS-relevant cell types. Methods: The toxicity and uptake profile of positively charged AmS-IONPs and negatively charged COOH-AmS-IONPs of similar size were examined using a mouse brain microvessel endothelial cell line (bEnd.3) and primary cultured mouse astrocytes and neurons. Cell accumulation of IONPs was examined using the ferrozine assay, and cytotoxicity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: No toxicity was observed in bEnd.3 cells at concentrations up to 200 µg/mL for either AmS-IONPs or COOH-AmS-IONPs. AmS-IONPs at concentrations above 200 µg/mL reduced neuron viability by 50% in the presence or absence of a magnetic field, while only 20% reductions in viability were observed with COOH-AmS-IONPs. Similar concentrations of AmS-IONPs in astrocyte cultures reduced viability to 75% but only in the presence of a magnetic field, while exposure to COOH-AmS-IONPs reduced viability to 65% and 35% in the absence and presence of a magnetic field, respectively. Cellular accumulation of AmS-IONPs was greater in all cell types examined compared to COOH-AmS-IONPs. Rank order of cellular uptake for AmS-IONPs was astrocytes . bEnd.3 . neurons. Accumulation of COOH-AmS-IONPs was minimal and similar in magnitude in different cell types. Magnetic field exposure enhanced cellular accumulation of both AmS-and COOH-AmS-IONPs. Conclusion: Both IONP compositions were nontoxic at concentrations below 100 µg/mL in all cell types examined. At doses above 100 µg/mL, neurons were more sensitive to AmS-IONPs, whereas astrocytes were more vulnerable toward COOH-AmS-IONPs. Toxicity appears to be dependent on the surface coating as opposed to the amount of iron-oxide present in the cell.

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“…Importantly, the geometry of GNPs, such as rods, shells, cages, or branched particles, not only impacts their LSPs but also alters their interaction with the bio-environment, and is thus critical to nanoparticle uptake and toxicity [17][18][19][20]. In addition to properties of the GNP, the cell type is also a determining factor in the bio-nano interaction [20].…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticles induce nuclear damage in breast cancer cells, which is further amplified by hyperthermia

Kodiha

Hutter

Boridy

et al. 2014

Cell. Mol. Life Sci.

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nanospheres, damaged the nucleus at normal growth temperature, several of these defects were further exacerbated by mild hyperthermia. Taken together, the toxicity of gold nanoparticles correlated with changes in nuclear organization and function. These results emphasize that the cell nucleus is a prominent target for gold nanoparticles of different morphologies. Moreover, we demonstrated that RNA synthesis in nucleoli provides quantitative information on nuclear damage and cancer cell survival.

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Microglial Response to Gold Nanoparticles

Abstract: See the accompanying Perspective by Albanese et al. on p 2490.

Cited by 266 publications

References 62 publications

Investigating the Role of Shape on the Biological Impact of Gold Nanoparticles in Vitro

Investigating the Role of Shape on the Biological Impact of Gold Nanoparticles in Vitro

Characterization of cellular uptake and toxicity of aminosilane-coated iron oxide nanoparticles with different charges in central nervous system-relevant cell culture models

Gold nanoparticles induce nuclear damage in breast cancer cells, which is further amplified by hyperthermia

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