Microglia modulate gliotransmission through the regulation of <scp>VAMP2</scp> proteins in astrocytes

Takata-Tsuji, Fuyuko; Chounlamountri, Naura; Do, Le-Duy; Philippot, Camille; Ducassou, Julia Novion; Couté, Yohann; Achour, Sarrah Ben; Honnorat, Jérôme; Place, Christophe; Pascual, Olivier

doi:10.1002/glia.23884

Cited by 10 publications

(6 citation statements)

References 39 publications

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“…Astrocytes closely interact and coordinate with microglia (e.g., synaptic pruning), and disruption of this signaling has been linked to various disease phenotypes ( Jha et al., 2019 ; Liddelow et al, 2020 ). Microglia can modulate astrocyte gliotransmission by regulating VAMP2 proteins ( Takata-Tsuji et al, 2021 ). Therefore, current evidence suggests that neurons, astrocytes, microglia, and OLCs co-operate multi-laterally to regulate neural circuit function.…”

Section: Challenges In Interpreting Astrocyte Functionmentioning

confidence: 99%

A perspective on astrocyte regulation of neural circuit function and animal behavior

Hirrlinger

Nimmerjahn

2022

Glia

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Studies over the past two decades have demonstrated that astrocytes are tightly associated with neurons and play pivotal roles in neural circuit development, operation, and adaptation in health and disease. Nevertheless, precisely how astrocytes integrate diverse neuronal signals, modulate neural circuit structure and function at multiple temporal and spatial scales, and influence animal behavior or disease through aberrant excitation and molecular output remains unclear. This Perspective discusses how new and state‐of‐the‐art approaches, including fluorescence indicators, opto‐ and chemogenetic actuators, genetic targeting tools, quantitative behavioral assays, and computational methods, might help resolve these longstanding questions. It also addresses complicating factors in interpreting astrocytes' role in neural circuit regulation and animal behavior, such as their heterogeneity, metabolism, and inter‐glial communication. Research on these questions should provide a deeper mechanistic understanding of astrocyte‐neuron assemblies' role in neural circuit function, complex behaviors, and disease.

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Section: Challenges In Interpreting Astrocyte Functionmentioning

confidence: 99%

A perspective on astrocyte regulation of neural circuit function and animal behavior

Hirrlinger

Nimmerjahn

2022

Glia

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“…Studying astrocytes in culture is a powerful way to clarify their function; however, due to the presence of contaminating cells, such as microglia in astrocyte cultures, it is difficult to determine if a cellular effect is truly cell autonomous. To assess the separate astroglial function, there are various techniques used to eliminate microglia, including AraC treatment alone, 63,64 LME treatment alone, 59,65 combining AraC and LME, 58 shaking alone, 66 shaking combined with AraC treatment, 67 or shaking combined with AraC and LME 68 …”

Section: Discussionmentioning

confidence: 99%

Differential production of interleukin‐6 and tumor necrosis factor‐α in primary rat astrocyte cultures using two distinct methods of microglia elimination

Barany

Tóth

Jocsak

et al. 2021

Clinical & Exp Neuroim

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Hepatic encephalopathy (HE) is a neurocognitive disorder due to acute or chronic liver failure as well as portosystemic bypass. Both neuroinflammation and oxidative stress have been strongly implicated in the pathogenesis of HE. An increasing body of evidence suggests that in the development of these mechanisms astrocytes may be involved. With regard of HE, this aspect of astrocytes is, however, neglected. While several studies focus on astrocytes related to the above‐mentioned pathological processes, the applied in vitro models are generated by diverse methods, which make the results difficult to compare with each other. In addition, these astrocyte cultures frequently contain microglia as well, complicating the interpretation of results. Therefore, we applied enriched primary rat astrocyte cultures generated by microglia elimination via shaking or the combination of cytosine β‐d‐arabinofuranoside hydrochloride (AraC) and l‐leucine methyl ester (LME). We examine whether some HE‐relevant pathological factors, such as ammonia, manganese (Mn), lipopolysaccharide (LPS), and H2O2 could elicit oxidative stress and cytokine production in astrocytes. Mn exposure increased the oxidative stress in astrocytes in a time‐dependent manner; furthermore, we could detect tumor necrosis factor (TNF)‐α production in both cultures at resting state. In addition, it was around 10‐fold higher in the cultures after shaking. LPS increased TNF‐α secretion in cultures after shaking, but not in cultures after AraC + LME treatment. In contrast, interleukin (IL)‐6 was detectable only in the AraC + LME‐treated cultures. In conclusion, we have demonstrated that HE‐relevant factors evoke oxidative stress and cytokine production, albeit depending on the elimination method used.

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“…Neurons require to interact with glial cells to maintain intercellular connectivity (Harada et al, 2016;Takata-Tsuji et al, 2021). Glial cells are the most numerous neural cell type in the CNS, regulating brain homeostasis through both the structural and functional support of neurons and their role as immune cells (Yi et al, 2015;Neto et al, 2016;Osaki et al, 2018a).…”

Section: Cell Culture Interactions In Microfluidic Devicesmentioning

confidence: 99%

Modeling Neurodegenerative Diseases Using In Vitro Compartmentalized Microfluidic Devices

Miny

Maisonneuve²,

Quadrio

et al. 2022

Front. Bioeng. Biotechnol.

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The human brain is a complex organ composed of many different types of cells interconnected to create an organized system able to efficiently process information. Dysregulation of this delicately balanced system can lead to the development of neurological disorders, such as neurodegenerative diseases (NDD). To investigate the functionality of human brain physiology and pathophysiology, the scientific community has been generated various research models, from genetically modified animals to two- and three-dimensional cell culture for several decades. These models have, however, certain limitations that impede the precise study of pathophysiological features of neurodegeneration, thus hindering therapeutical research and drug development. Compartmentalized microfluidic devices provide in vitro minimalistic environments to accurately reproduce neural circuits allowing the characterization of the human central nervous system. Brain-on-chip (BoC) is allowing our capability to improve neurodegeneration models on the molecular and cellular mechanism aspects behind the progression of these troubles. This review aims to summarize and discuss the latest advancements of microfluidic models for the investigations of common neurodegenerative disorders, such as Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis.

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Microglia modulate gliotransmission through the regulation of VAMP2 proteins in astrocytes

Cited by 10 publications

References 39 publications

A perspective on astrocyte regulation of neural circuit function and animal behavior

A perspective on astrocyte regulation of neural circuit function and animal behavior

Differential production of interleukin‐6 and tumor necrosis factor‐α in primary rat astrocyte cultures using two distinct methods of microglia elimination

Modeling Neurodegenerative Diseases Using In Vitro Compartmentalized Microfluidic Devices

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