Microgel immunoblotting of thymus and nuclear extracts by unidirectional diffusion

Keyser, Filip De; Verbruggen, G.; Em, Veys; Luyten, Frank P.; Segers, J.; Rabaey, M

doi:10.1016/0003-2697(89)90321-7

Cited by 4 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Diffusion blotting is also possible from dense gels if they are ultrathin (10). The arnount of antibody bound must therefore depend on the particular set of antibodies and antigen conformations present during incubation.…”

Section: Princlple Of the Methodsmentioning

confidence: 99%

Review

1989

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

Since its introduction in 1979 protein blotting has become a routine tool in research laboratories. In the clinical laboratory its potential is becoming recognised for applications in fields such äs infectious and autoimmune diseases, allergy and others. In this article we would like to outline the basic principles of protein blotting, to illustrate these with soine examples related to clinical applications and to point out differences between these and classical methods.Immunoblot analysis: a new method for fingerprinting hospital pathogens Characterization of autoantigens and autoantibodies by immunoblotting Characterisation of allergens by protein blotting

show abstract

Section: Princlple Of the Methodsmentioning

confidence: 99%

Review

1989

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

show abstract

Antinuclear antibody determination methods

et al. 1990

View full text Add to dashboard Cite

Screening rheumatology patients for anti-nuclear and anti-cytoplasmic antibodies is easily done in a qualitative manner using the IF, CIE and ID assays. The immunoblot is of use for anti-La and anti-RNP assays but gives anomalous results for Sm binding by anti-RNP sera and is not easily quantitated. These deficiencies of the immunoblot do not apply to the ELISA. Advances in cloning of autoantigens will enable standardisation of antigen preparations used for these ELISAs. The quantitation of autoantibody appears significant since disease flares occur together with elevations in specific autoantibody. IgM anti-Sm autoantibody was detected with a different disease distribution to IgG anti-Sm but the prognostic implications for this remain to be determined.

show abstract