Microfocus X-Ray Method for Detecting Hidden Defects in Seeds of Woody Forest Species and Other Types of Vascular Plants

Arkhipov, Mikhail; Priyatkin, N. S.; Гусакова, Л. П.; Карамышева, А. В.; Trofimuk, L. P.; Потрахов, Н. Н.; Bessonov, V. B.; Shchukina, P. A.

doi:10.1134/s1063784220020024

Cited by 7 publications

(9 citation statements)

References 9 publications

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“…The use of the X-ray technique was satisfactory to identify these characteristics in a non-destructive, precise, and fast way. Other works have also successfully used the technique for analyzing seeds of forest species such as Tabebuia heptaphylla (Amaral et al, 2011), Acca sellowiana (Silva et al, 2013), Terminalia argentea (Gomes et al, 2014), Platypodium elegans (Gomes et al, 2016), Senna multijuga (Marchi and Gomes-Junior, 2017), Moringa oleifera (Noronha et al, 2018), Leucaena leucocephala (Medeiros et al, 2018), Lecythis pisonis (Rosa et al, 2020), Arctostaphylos pungens, Juniperus deppeana (Rubalcava-Castillo et al, 2020), and Pinus sibirica (Arkhipov et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

“…Variables based on pixel intensity, such as relative density, integrated density, and median gray, have been used to classify seeds within physical quality levels (Abud et al, 2018;Arkhipov et al, 2020;Medeiros et al, 2018). These variables are calculated using the gray values of each pixel in the image and have a direct relationship to the resistance that the internal tissues of the seed present to the X-ray passage (Kotwaliwale et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

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Seed quality analysis of Senna siamea Lam. using image analysis techniques

et al. 2020

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The inclusion of image analysis techniques for the accurate and rapid assessment of the quality of forest seeds is highly desirable. The use of digital radiographic images of seeds and the analysis of seedling images, still little used in determining the quality of forest seeds, are among the available imaging techniques. This study aimed to assess the feasibility of using the analysis of images of seeds and seedlings in the assessment of the physical and physiological quality of Senna siamea seeds. Radiographic images of seeds were obtained from five seed lots, allowing generating morphological and tissue integrity descriptors. These seeds were then subjected to germination and seedling growth tests, which allowed obtaining variables related to physiological quality. The generated seedlings were scanned and analyzed using the software ImageJ. The data were analyzed using analysis of variance, correlation, and principal component analysis. The results showed differences between seed lots in terms of physiological quality and physical integrity of internal tissues. Significant correlations were observed between the variables obtained with the radiographic analysis and seed physiological characterization tests. The use of techniques to analyze seed radiographs and seedling images allows access to information on the physical and physiological integrity of S. siamea seeds.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Seed quality analysis of Senna siamea Lam. using image analysis techniques

et al. 2020

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“…The study of the internal structure of the seeds was carried out by the method of digital microfocus radiography. This method is included in international standards, primarily for assessing the infestation and damage of grain by pests [9,10]. The PRDU unit has a protective chamber for X-ray imaging, a monoblock X-ray source RAP70M-0.1N-1, an Xray image receiver based on a multifunctional portable flat-panel X-ray detector ViVIX-S for digital radiography.…”

Section: Methodsmentioning

confidence: 99%

“…The control is carried out from a computerized console with the MicroCT-PRDU universal software for analyzing digital X-ray images of seeds. The installation can obtain images with a geometric magnification of 3.0 × [10]. The following parameters are adjusted in the MicroCT-PRDU software before the examination: anode voltage, exposure time.…”

Section: Methodsmentioning

confidence: 99%

“…includes a unique X-ray diagnostic unit for non-damaging transillumination of seeds PRDU, devices for assessing moisture, mass, studying the morphology of seeds, laboratory refrigerating chambers for their storage. The PRDU device, developed by native specialists, is successfully used not only to assess the quality of seeds of food crops, but also to study seeds of various plants by specialists in botanical gardens [9][10][11]. This report presents the preliminary results of the X-ray study of the seeds of some rare plants of the Samara region…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the quality of seeds formed in situ and ex situ as a mandatory element of maintaining seed banks of rare plants

et al. 2021

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The possibility of seed genetic banks creating is associated with the ability of many plants seeds (the so-called orthodox ones) to maintain germination ability for a long time, being in a dormant state. This opens up wide prospects for the formation of a reserve seeds fund of rare species that are threatened with extinction in natural communities. Botanical gardens in different countries of the world, including the Russian Federation, are working on the creation of such seed banks. To assess the quality of seeds in relation to agricultural crops, as well as natural flora species, radiography is used. It is this kind of non-damaging express assessment of the quality of seeds that turns out to be the most informative technique for seed material obtained in botanical gardens. The article presents the preliminary results of an X-ray study of the seeds quality of some Samara region rare plants - four species ofthe Iris genus - Iris aphylla L., I. halophila Pall., I. pumila L., I. sibirica L.

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EVALUATION OF HETEROGENEITY AND HIDDEN DEFECTS OF WHEAT (Triticum aestivum L.) SEEDS BY INSTRUMENTAL PHYSICAL METHODS

Priyatkin¹

2022

S-h. biol.

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Для контроля качества семенного материала существует ряд стандартных тестов, регламентированных ISTA (International Seed Testing Association, Швейцария), а также перспективных инструментальных методов, оценивающих характеристики поверхности семян, их структурной целостности и интегральные электрофизические параметры. Цель исследования заключалась в оценке эффективности инструментальных физических методов при изучении скрытой дефектности у экологически разнокачественных семян пшеницы разного генетического происхождения. Разнокачественность и скрытую дефектность семян пшеницы (Triticum aestivum L.) оценивали с использованием оптической визуализации, микрофокусной рентгенографии и электрофотографии. Установлено, что метод оптической визуализации в сочетании с автоматическим анализом цифровых сканированных изображений позволяет статистически достоверно различать семена пшеницы разных сортов и генетических линий по цветовым характеристикам -составляющим цветовой модели RGB (red -красный, green -зеленый, blue -синий), а именно цветовому тону и насыщенности. Также выявлены различия между семенами одних и тех же сортообразцов и генетических линий, полученных в полевых и регулируемых условиях. Обнаружено, что цветовые характеристики поверхности семян, оцениваемые с помощью анализа цифровых сканированных изображений, статистически значимо различаются как у сортов и генетических линий пшеницы, так и у одного образца, выращенного в неодинаковых экологических условиях (полевых либо регулируемых). В частности, значения цветового тона цифровых сканированных изображений у семян разных сортов пшеницы, полученных в регулируемых условиях (фитополигон Агрофизического института), менялись от 0

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Microfocus X-Ray Method for Detecting Hidden Defects in Seeds of Woody Forest Species and Other Types of Vascular Plants

Cited by 7 publications

References 9 publications

Seed quality analysis of Senna siamea Lam. using image analysis techniques

Seed quality analysis of Senna siamea Lam. using image analysis techniques

Assessment of the quality of seeds formed in situ and ex situ as a mandatory element of maintaining seed banks of rare plants

EVALUATION OF HETEROGENEITY AND HIDDEN DEFECTS OF WHEAT (Triticum aestivum L.) SEEDS BY INSTRUMENTAL PHYSICAL METHODS

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