Microfluidics using a thiol–acrylate resin for fluorescence-based pathogen detection assays

Zhang, W.; Tullier, Michael; Patel, Kush; Carranza, Arturo; Radadia, Adarsh D.

doi:10.1039/c5lc00971e

Cited by 8 publications

(12 citation statements)

References 38 publications

(39 reference statements)

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“…Thus, a thiol-acrylate resin that exhibits low background fluorescence was reported for making microfluidic devices at room temperature [28]. Simple electrostatic interaction between the channel walls and an Ab to E. coli was used for immobilization.…”

Section: On-chip Sample Preparation Methodsmentioning

confidence: 99%

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

Sonker

Sahore

Woolley

2017

Analytica Chimica Acta

138

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Microfluidics is a vibrant and expanding field that has the potential for solving many analytical challenges. Microfluidics show promise to provide rapid, inexpensive, efficient, and portable diagnostic solutions that can be used in resource-limited settings. Researchers have recently reported various microfluidic platforms for biomarker analysis applications. Sample preparation processes like purification, preconcentration and labeling have been characterized on-chip. Additionally, improvements in microfluidic separation techniques have been reported for cellular and molecular biomarkers. This review critically evaluates microfluidic sample preparation platforms and separation methods for biomarker analysis reported in the last two years. Key advances in device operation and ability to process different sample matrices in a variety of device materials are highlighted. Finally, current needs and potential future directions for microfluidic device development to realize its full diagnostic potential are discussed.

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Section: On-chip Sample Preparation Methodsmentioning

confidence: 99%

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

Sonker

Sahore

Woolley

2017

Analytica Chimica Acta

138

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“… 22 , 23 Materials such as poly(methyl methacrylate), polycarbonate, and cyclic olefins copolymers (COC/COP) have been utilized in developing microfluidic devices; however, each of these materials has limitations in the abovementioned criteria. 22 − 25 Recent work by Bounds et al and Tullier et al aimed to overcome some of these challenges by developing a novel polymeric material to replace PDMS using thiol-ene chemistry, 23 , 25 , 26 which allows for polymerization by applying UV-light (photopolymerization) 27 , 28 or by a base-catalyzed Michael addition. 23 , 26 , 29 , 30 Both of these techniques resulted in highly cross-linked thermoset polymers in short time scales (∼30 min) with close to 100% monomer conversion.…”

Section: Introductionmentioning

confidence: 99%

“…Unlike PDMS, TAMR is hydrophilic and both the TAMR and hydrogel surface can be modified relatively easily compared to PDMS. 23 , 25 This is because both the TAMR and hydrogel are made with thiol and acrylate monomers which can be functionalized with different groups in time of need. The microfluidic or rigid PDMS-like TAMR contains negatively imprinted channels.…”

Section: Introductionmentioning

confidence: 99%

“…The microfluidic or rigid PDMS-like TAMR contains negatively imprinted channels. 23 , 25 The diffusive layer (or porous hydrogel, H) was synthesized to facilitate the passive diffusion of biomolecules. The TAMR/H system was compared to a control, flow-free microfluidic device consisting of a fluidic channel imprinted into PDMS coupled with an agarose slab encased in a Plexiglas chamber.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Flow-free Gradient Generator Using a Self-Adhesive Thiol-acrylate Microfluidic Resin/Hydrogel (TAMR/H) Hybrid System

Khan

Smith

Tullier

et al. 2021

ACS Appl. Mater. Interfaces

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Microfluidic gradient generators have been used to study cellular migration, growth, and drug response in numerous biological systems. One type of device combines a hydrogel and polydimethylsiloxane (PDMS) to generate “flow-free” gradients; however, their requirements for either negative flow or external clamps to maintain fluid-tight seals between the two layers have restricted their utility among broader applications. In this work, a two-layer, flow-free microfluidic gradient generator was developed using thiol-ene chemistry. Both rigid thiol-acrylate microfluidic resin (TAMR) and diffusive thiol-acrylate hydrogel (H) layers were synthesized from commercially available monomers at room temperature and pressure using a base-catalyzed Michael addition. The device consisted of three parallel microfluidic channels negatively imprinted in TAMR layered on top of the thiol-acrylate hydrogel to facilitate orthogonal diffusion of chemicals to the direction of flow. Upon contact, these two layers formed fluid-tight channels without any external pressure due to a strong adhesive interaction between the two layers. The diffusion of molecules through the TAMR/H system was confirmed both experimentally (using fluorescent microscopy) and computationally (using COMSOL). The performance of the TAMR/H system was compared to a conventional PDMS/agarose device with a similar geometry by studying the chemorepulsive response of a motile strain of GFP-expressing Escherichia coli . Population-based analysis confirmed a similar migratory response of both wild-type and mutant E. coli in both of the microfluidic devices. This confirmed that the TAMR/H hybrid system is a viable alternative to traditional PDMS-based microfluidic gradient generators and can be used for several different applications.

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“…30 In each case though, the higher the catalyst/initiator concentration, the faster the reaction will proceed. Applications of thiol-acrylate chemistry range from coupling reactions between monofunctional species [31][32][33] to the fabrication of microparticles, 34 polymer materials for microfluidics, 35,36 biocompatible tissue scaffolds, 37,38 hydrogels, 39,40 and polymers with a variety of architectures. [41][42][43][44]…”

mentioning

confidence: 99%

The effect of a crosslinking chemical reaction on pattern formation in viscous fingering of miscible fluids in a Hele–Shaw cell

Bunton

Tullier

Meiburg

2017

Chaos: An Interdisciplinary Journal of Nonlinear Science

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Viscous fingering can occur in fluid motion whenever a high mobility fluid displaces a low mobility fluid in a Darcy type flow. When the mobility difference is primarily attributable to viscosity (e.g., flow between the two horizontal plates of a Hele-Shaw cell), viscous fingering (VF) occurs, which is sometimes termed the Saffman-Taylor instability. Alternatively, in the presence of differences in density in a gravity field, buoyancy-driven convection can occur. These instabilities have been studied for decades, in part because of their many applications in pollutant dispersal, ocean currents, enhanced petroleum recovery, and so on. More recent interest has emerged regarding the effects of chemical reactions on fingering instabilities. As chemical reactions change the key flow parameters (densities, viscosities, and concentrations), they may have either a destabilizing or stabilizing effect on the flow. Hence, new flow patterns can emerge; moreover, one can then hope to gain some control over flow instabilities through reaction rates, flow rates, and reaction products. We report effects of chemical reactions on VF in a Hele-Shaw cell for a reactive step-growth cross-linking polymerization system. The cross-linked reaction product results in a non-monotonic viscosity profile at the interface, which affects flow stability. Furthermore, three-dimensional internal flows influence the long-term pattern that results.

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Microfluidics using a thiol–acrylate resin for fluorescence-based pathogen detection assays

Cited by 8 publications

References 38 publications

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

Development of a Flow-free Gradient Generator Using a Self-Adhesive Thiol-acrylate Microfluidic Resin/Hydrogel (TAMR/H) Hybrid System

The effect of a crosslinking chemical reaction on pattern formation in viscous fingering of miscible fluids in a Hele–Shaw cell

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