Microfluidics generation of chitosan microgels containing glycerylphytate crosslinker for in situ human mesenchymal stem cells encapsulation

Mora‐Boza, Ana; Castro, Lina M. Mancipe; Schneider, Rebecca; Han, Woojin M.; Garcı́a, Andrés J.; Vázquez‐Lasa, Blanca; Román, Julio San

doi:10.1016/j.msec.2020.111716

Cited by 21 publications

(19 citation statements)

References 55 publications

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“…Birçok mikroakışkan kültür sistemi, hücreleri 2B kültürlemek için tasarlanmıştır; ancak, son zamanlarda mikroakışkan cihazlarda hücrelerin doğal fenotipini 3B ortamlarda taklit etmek için çalışmalar yapılmaktadır [62]. 3B ECM ve hidrojel sistemleri, mikroakışkan sistemlerinde bu amaç için, örneğin damlacık metodu kullanılarak uygulanmaktadır [66]. Bu yöntemde hücreler çeşitli hidrojel yapıları içerisine mikroakışkan platform tarafından kontrollü şekilde yüklenir [67].…”

Section: 1unclassified

Mikroakışkan Çiplere Kök Hücre ve Doku Mühendisliği Perspektifinden Bakış

Torkay

Bal‐Öztürk

2024

Politeknik Dergisi

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Kolayca modifiye edilebilir ve pek çok çalışmaya entegre edilebilir özellikleriyle mikroakışkan sistemler son yıllarda araştırmacıların ilgi odağındadır. Mikroakışkan çipler sayesinde daha az solüsyon ve sürekli perfüzyon ile kontrollü ve optimize hücre kültürü çalışmaları yapılabilmektedir. Son yıllarda özellikle rejeneratif tıbbın ilgisini çeken kök hücrelerin tek başına veya diğer hücrelerle birlikte kültürlenmesi ve kullanılan kök hücrelerin istenilen yönde farklılaştırılması çip sistemlerinde sıklıkla çalışılmaktadır. Bu sistemlere hücreler arası ortam koşullarını taklit edecek hidrojellerin veya hücrelerinden arındırılmış organ matrislerinin de ilave edilmesi in vivo'ya daha yakın sonuçlar vermektedir. Çiplerin üretildiği malzeme, yüzey modifikasyonları, akış hızı, besi yeri içeriği, kullanılan hidrojellerin mekano-kimyasal özellikleri, elektriksel, kimyasal ya da mekanik uyarımlar neticesinde kök hücrelerin farklılaşmaları da dahil tüm davranışlarının oldukça değiştiğini gösteren birçok çalışma mevcuttur. Mikroakışkan çip sistemlerinin ilerleyen zamanlarda kişiselleştirilmiş tıp, ilaç toksisite deneyleri, hasta-yanı hızlı tanı kitleri ve birçok temel bilim araştırmasına yeni bir boyut kazandıracağı, özellikle hayvan deneylerinin yerini alarak daha güvenilir ve ucuz potansiyel yöntemlerin başında geleceği öngörülmektedir. Tüm bu sebepler çip sistemlerini araştırma odağı yapmaktadır. Bu çalışmada; mikroakışkan çip sistemlerinin üretimi, avantajları, dezavantajları ve doku mühendisliği alanındaki uygulamaları tartışılmıştır.

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Section: 1unclassified

Mikroakışkan Çiplere Kök Hücre ve Doku Mühendisliği Perspektifinden Bakış

Torkay

Bal‐Öztürk

2024

Politeknik Dergisi

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“…The most frequently used geometry configurations to generate the droplets in the devices are T-junction, flow-focusing, and co-flowing (or capillary) laminar streams, which are illustrated in Figure 1C [41][42][43][44]. Microgels are especially attractive as cell carriers, because their large surface-to-volume ratio promotes efficient mass transport and enhances cell-matrix interactions, but it is important to notice that cell microencapsulation requires a polymer network that ensures cell viability during microgel preparation and adequate crosslinking chemistry to form a polymer network [45]. Microfluidics technology provides a tight control over microgel chemical properties and composition by easily tuning the flow rates and components in the microfluidic channels, being a versatile biofabrication platform, where different crosslinking strategies can be applied [41].…”

Section: Microfluidicsmentioning

confidence: 99%

“…Encapsulation of MSCs in polymeric microgels is an excellent approach to improving cell persistence and immunomodulation [61]. Cell therapies based on MSCs are particularly interesting in ameliorating immune-related diseases and dysregulations, but they are limited due to short in vivo persistence [44,45,61,62]. Mao et al [61] reported the encapsulation of MSCs in alginate-polylysine microgels using a microfluidic device.…”

Section: Figurementioning

confidence: 99%

“…In addition, the crosslinking methodology allowed the encapsulation of NIH-3T3 fibroblasts, which showed high viability after two days of culture, demonstrating the biocompatibility of the entire process. Mora-Boza et al [45] reported the fabrication of hMSCs-laden microgels, applying also an in situ crosslinking approach for chitosan lactate (ChLA), a water-soluble chitosan derivative. The ionotropic gelation was based on a combination of glycerylphytate (G 1 Phy) and tripolyphosphate (TPP) as ionic crosslinkers, obtaining polymeric microgels with homogeneous size distribution between 104 and 127 µm.…”

Section: Chitosanmentioning

confidence: 99%

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Emerging Biofabrication Techniques: A Review on Natural Polymers for Biomedical Applications

2021

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Natural polymers have been widely used for biomedical applications in recent decades. They offer the advantages of resembling the extracellular matrix of native tissues and retaining biochemical cues and properties necessary to enhance their biocompatibility, so they usually improve the cellular attachment and behavior and avoid immunological reactions. Moreover, they offer a rapid degradability through natural enzymatic or chemical processes. However, natural polymers present poor mechanical strength, which frequently makes the manipulation processes difficult. Recent advances in biofabrication, 3D printing, microfluidics, and cell-electrospinning allow the manufacturing of complex natural polymer matrixes with biophysical and structural properties similar to those of the extracellular matrix. In addition, these techniques offer the possibility of incorporating different cell lines into the fabrication process, a revolutionary strategy broadly explored in recent years to produce cell-laden scaffolds that can better mimic the properties of functional tissues. In this review, the use of 3D printing, microfluidics, and electrospinning approaches has been extensively investigated for the biofabrication of naturally derived polymer scaffolds with encapsulated cells intended for biomedical applications (e.g., cell therapies, bone and dental grafts, cardiovascular or musculoskeletal tissue regeneration, and wound healing).

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“…Known for its cross-linking and non-toxic properties, tripolyphosphate (TPP) is very often found in CS hydrogels [ 126 , 133 ] and NPs [ 134 , 135 ]. TPP can have up to five negative charges and is believed to align along the CS chains in a gel, creating sheets of CS-TPP [ 136 ].…”

Section: Non-covalent Modificationsmentioning

confidence: 99%

Chitosan Functionalization: Covalent and Non-Covalent Interactions and Their Characterization

2021

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Chitosan (CS) is a natural biopolymer that has gained great interest in many research fields due to its promising biocompatibility, biodegradability, and favorable mechanical properties. The versatility of this low-cost polymer allows for a variety of chemical modifications via covalent conjugation and non-covalent interactions, which are designed to further improve the properties of interest. This review aims at presenting the broad range of functionalization strategies reported over the last five years to reflect the state-of-the art of CS derivatization. We start by describing covalent modifications performed on the CS backbone, followed by non-covalent CS modifications involving small molecules, proteins, and metal adjuvants. An overview of CS-based systems involving both covalent and electrostatic modification patterns is then presented. Finally, a special focus will be given on the characterization techniques commonly used to qualify the composition and physical properties of CS derivatives.

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Microfluidics generation of chitosan microgels containing glycerylphytate crosslinker for in situ human mesenchymal stem cells encapsulation

Cited by 21 publications

References 55 publications

Mikroakışkan Çiplere Kök Hücre ve Doku Mühendisliği Perspektifinden Bakış

Mikroakışkan Çiplere Kök Hücre ve Doku Mühendisliği Perspektifinden Bakış

Emerging Biofabrication Techniques: A Review on Natural Polymers for Biomedical Applications

Chitosan Functionalization: Covalent and Non-Covalent Interactions and Their Characterization

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