Microfluidics for interrogating live intact tissues

Horowitz, Lisa F.; Rodriguez, A. D.; Ray, Tyler R.; Folch, Albert

doi:10.1038/s41378-020-0164-0

Cited by 35 publications

(24 citation statements)

References 213 publications

(206 reference statements)

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“…For instance, it has been beneficial for high-throughput Arabidopsis root research [96]. Remarkably, the growth fashion of the pollen tube and the embryo development in Arabidopsis or the protonemal growth and early gametophore development of P. patens are ideal candidates for high-throughput and high-resolution imaging in microfluidic devices with lightand fluorescence-based microscopies [93,97].…”

Section: Microfluidicsmentioning

confidence: 99%

Mosses: Accessible Systems for Plant Development Studies

Floriach-Clark¹,

Tang²,

Willemsen³

2022

Model Organisms in Plant Genetics

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Mosses are a cosmopolitan group of land plants, sister to vascular plants, with a high potential for molecular and cell biological research. The species Physcomitrium patens has helped gaining better understanding of the biological processes of the plant cell, and it has become a central system to understand water-to-land plant transition through 2D-to-3D growth transition, regulation of asymmetric cell division, shoot apical cell establishment and maintenance, phyllotaxis and regeneration. P. patens was the first fully sequenced moss in 2008, with the latest annotated release in 2018. It has been shown that many gene functions and networks are conserved in mosses when compared to angiosperms. Importantly, this model organism has a simplified and accessible body structure that facilitates close tracking in time and space with the support of live cell imaging set-ups and multiple reporter lines. This has become possible thanks to its fully established molecular toolkit, with highly efficient PEG-assisted, CRISPR/Cas9 and RNAi transformation and silencing protocols, among others. Here we provide examples on how mosses exhibit advantages over vascular plants to study several processes and their future potential to answer some other outstanding questions in plant cell biology.

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Section: Microfluidicsmentioning

confidence: 99%

Mosses: Accessible Systems for Plant Development Studies

Floriach-Clark¹,

Tang²,

Willemsen³

2022

Model Organisms in Plant Genetics

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“… 78 , 79 Owing to the laminar flow regime in microchannels, multiple hormones can be delivered to selected regions simultaneously. Two recent reviews discuss various microfluidic devices for plant biology applications, 79 , 80 while here we will highlight microfluidics for local hormone/chemical delivery.…”

Section: Devices For Modulating Plant Physiologymentioning

confidence: 99%

Plant Bioelectronics and Biohybrids: The Growing Contribution of Organic Electronic and Carbon-Based Materials

et al. 2021

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Life in our planet is highly dependent on plants as they are the primary source of food, regulators of the atmosphere, and providers of a variety of materials. In this work, we review the progress on bioelectronic devices for plants and biohybrid systems based on plants, therefore discussing advancements that view plants either from a biological or a technological perspective, respectively. We give an overview on wearable and implantable bioelectronic devices for monitoring and modulating plant physiology that can be used as tools in basic plant science or find application in agriculture. Furthermore, we discuss plant-wearable devices for monitoring a plant’s microenvironment that will enable optimization of growth conditions. The review then covers plant biohybrid systems where plants are an integral part of devices or are converted to devices upon functionalization with smart materials, including self-organized electronics, plant nanobionics, and energy applications. The review focuses on advancements based on organic electronic and carbon-based materials and discusses opportunities, challenges, as well as future steps.

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“…22 In the future, additional microfluidic modules can be added to the CoreView millifluidic chip for maintaining vitality of fresh tissue for realtime chemical monitoring, reactions to applied chemotherapy drugs, and analysis of extracted analytes from CNBs. [23][24][25] The ability of CoreView to produce histopathologic images in minutes instead of hours to days presents a major global health solution. Breast cancer is the most common cancer and secondleading cause of cancer-related deaths in sub-Saharan Africa.…”

Section: Implications For the Future Of Pathologymentioning

confidence: 99%

CoreView: Fresh Tissue Biopsy Assessment at the Bedside Using a Millifluidic Imaging Chip

Cooper¹,

Huang²,

Klavins³

et al. 2021

Preprint

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Minimally invasive core needle biopsies for medical diagnoses have become increasingly common for many diseases. Although tissue cores can yield more diagnostic information than fine needle biopsies and cytologic evaluation, there is no rapid evaluation at the point-of-care for intact tissue cores that is low-cost and non-destructive to the biopsy. We have developed a proof-of-concept 3D printed millifluidic histopathology lab-on-a-chip device to automatically handle, process, and image fresh core needle biopsies. This device, named CoreView, includes modules for biopsy removal from the acquisition tool, transport, staining and rinsing, imaging, segmentation, and multiplexed storage. Reliable removal from side-cutting needles and bidirectional fluid transport of core needle biopsies from five tissue types has been demonstrated with 0.5-mm positioning accuracy. Automation is aided by a MATLAB-based biopsy tracking algorithm that can detect the location of tissue and air bubbles in the channels of the millifluidic chip. With current and emerging optical imaging technologies, CoreView can be used for a rapid adequacy test at the point-of-care for tissue identification and glomeruli counting from renal core needle biopsies, and has the potential to be used for breast cancer diagnosis, phenotyping, and mapping of enriched tumorous regions for downstream analyses in global health applications.<br>

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Microfluidics for interrogating live intact tissues

Cited by 35 publications

References 213 publications

Mosses: Accessible Systems for Plant Development Studies

Mosses: Accessible Systems for Plant Development Studies

Plant Bioelectronics and Biohybrids: The Growing Contribution of Organic Electronic and Carbon-Based Materials

CoreView: Fresh Tissue Biopsy Assessment at the Bedside Using a Millifluidic Imaging Chip

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