Microfluidics for Electrophysiology, Imaging, and Behavioral Analysis of<i>Hydra</i>

Badhiwala, Krishna N.; Gonzales, D.; Vercosa, Daniel G.; Avants, Benjamin W.; Robinson, Jacob T.

doi:10.1101/257691

Cited by 5 publications

(26 citation statements)

References 79 publications

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“…Bioelectronic platforms combined with calcium imaging can also be applied to other transparent, millimeter-sized animals such as Hydra ( Figure 3C) (Badhiwala et al, 2018;Tzouanas et al, 2019). As is the case for C. elegans, studies of Hydra may help researchers to understand how neural circuits distributed throughout the nervous system regulate simple behaviors such as locomotion and feeding ( Figure 3C).…”

Section: Bioelectronic Applicationsmentioning

confidence: 99%

“…Although electrophysiological and imaging modalities have already been discussed, these recordings can be combined with environmental manipulations such as chemical delivery, temperature regulation, and mechanical stimuli (Badhiwala et al, 2018;Cho and Sternberg, 2014;Han et al, 2017;Kopito and Levine, 2014;Larsch et al, 2015;Lockery et al, 2012;McClanahan et al, 2017;Nekimken et al, 2017;Swierczek et al, 2011;Tzouanas et al, 2019). For example, the substance reduced glutathione (GSH) can be delivered to Hydra with precise temporal control to induce mouth opening, which can be simultaneously imaged with GCaMP indicators ( Figures 3C and 3F) (Badhiwala et al, 2018). Likewise, as previously mentioned, chemicals can be delivered in real-time to multiple nematodes during pharyngeal recordings to discover more potent anthelmintic drugs ( Figure 3E) (Lockery et al, 2012;Weeks et al, 2016) or screen for drugs to treat diseases.…”

Section: Combining Bioelectronics With Other Interrogation Modalitiesmentioning

confidence: 99%

“…microfabricated devices with throughputs of at least 20-1000 animals/day for C. elegans have been developed that monitor behavior (Albrecht and Bargmann, 2011;Churgin et al, 2017;Hulme et al, 2010;Swierczek et al, 2011), record single-neuron calcium activity (Chokshi et al, 2010;Chronis et al, 2007;Larsch et al, 2013), detect synaptic morphological changes (Chung et al, 2008;Crane et al, 2012), record electrophysiological activity (Gonzales et al, 2017;Hu et al, 2013;Lockery et al, 2012), and screen for drug efficacy and side effects (Cornaglia et al, 2016;Mondal et al, 2016). Likewise, similar technologies fabricated at the microscale have been developed for Hydra (Badhiwala et al, 2018;Dexter et al, 2015), drosophila larvae (Bernstein et al, 2004;Chung et al, 2011;Levario et al, 2013;Lucchetta et al, 2006), and zebrafish (Chang et al, 2012;Pardo-Martin et al, 2013 for monitoring the behavior of animals with a throughput reaching tens of animals/days.…”

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confidence: 99%

“…Panel A (top) adapted from Lockery et al (2012) with permission from the Royal Society of Chemistry and (bottom) adapted from Raizen and Avery (1994). Panel B (top) adapted from Passano and McCullough (1964) with permission from The Company of Biologists and (bottom) adapted from Badhiwala et al (2018). Panel C (top) adapted from Gao and Zhen (2011) and (bottom) adapted from Gonzales et al (2017).…”

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Bioelectronics for Millimeter-Sized Model Organisms

et al. 2020

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Advances in microfabrication technologies and biomaterials have enabled a growing class of electronic devices that can stimulate and record bioelectronic signals. Many of these devices have been developed for humans or vertebrate animals, where miniaturization allows for implantation within the body. There are, however, another class of bioelectronic interfaces that exploit microfabrication and nanoelectronics to record signals from tiny, millimeter-sized organisms. In these cases, rather than implanting a device inside an animal, animals themselves are loaded in large numbers into bioelectronic devices for neural circuit and behavioral interrogation. These scalable interfaces provide platforms to develop new therapeutics as well as better understand basic principles of bioelectronic communication, neuroscience, and behavior. Here we review recent progress in these bioelectronic technologies and describe how they can complement on-chip optical, mechanical, and chemical interrogation methods to achieve high-throughput, multimodal studies of millimeter-sized small animals. ADVANTAGES OF MILLIMETER-SIZED ORGANISMS Small model organisms such as Caenorhabditis elegans, Drosophila, and zebrafish have been a cornerstone for understanding principles of neurobiology, behavior, genetics, and development (Davis, 2004; Sattelle and Buckingham, 2006; Stewart et al., 2014). Despite their small nervous systems and less anatomical complexity compared with that of mammalian models, there are significant advantages in using millimeter-sized animals to answer fundamental questions in neurobiology (Figure 1).

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Section: Bioelectronic Applicationsmentioning

confidence: 99%

Section: Combining Bioelectronics With Other Interrogation Modalitiesmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Bioelectronics for Millimeter-Sized Model Organisms

et al. 2020

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“…Their neurons continually replenished and are more dynamic than in other model organisms (Badhiwala, Gonzales, Vercosa, Avants, & Robinson, 2018). Badhiwala et al (2018) presented three polymer-based microfluidic devices to study the fragile Hydra : (1) an hour-glass chamber for electrophysiology that constrained the body column from large movements, (2) a wheel-and-spoke perfusion chamber that constrained locomotion, and (3) a behavioral micro-arena with micropillars that limited movements and locomotion in the vertical plane but allowed all movements in the horizontal plane. They observed that the Hydra reacted to shear stress induced by a high flow rate with body contractions or tentacle swaying.…”

Section: Microfluidics For Mechanobiology Of Model Organismsmentioning

confidence: 99%

Microfluidics for mechanobiology of model organisms

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Nekimken

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et al. 2018

Methods in Cell Biology

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Mechanical stimuli play a critical role in organ development, tissue homeostasis, and disease. Understanding how mechanical signals are processed in multicellular model systems is critical for connecting cellular processes to tissue- and organism-level responses. However, progress in the field that studies these phenomena, mechanobiology, has been limited by lack of appropriate experimental techniques for applying repeatable mechanical stimuli to intact organs and model organisms. Microfluidic platforms, a subgroup of microsystems that use liquid flow for manipulation of objects, are a promising tool for studying mechanobiology of small model organisms due to their size scale and ease of customization. In this work, we describe design considerations involved in developing a microfluidic device for studying mechanobiology. Then, focusing on worms, fruit flies, and zebrafish, we review current microfluidic platforms for mechanobiology of multicellular model organisms and their tissues and highlight research opportunities in this developing field.

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