Microfluidics for Biomedical Applications

Abstract

Microfluidics refers to a technique for controlling and analyzing the fluids or micro-/nano-bioparticles in microscale channels or structures [...]

Addressing Key Questions in Organoid Models: Who, Where, How, and Why?

“…The development of 3D in vitro culture systems that maintain the original characteristics of the distinct tissues and organs within the female reproductive tract has progressed substantially from 2017, when two separate groups described the first human endometrial organoids [22,73], to 2021, when Rawlings et al introduced assembloid models combining multiple cell types in the same 3D culture [19]. Nevertheless, static 3D culture still has several limitations, such as the lack of mechanical flow dynamics, poor connectivity with other cell culture wells, challenging pH and temperature standardization, and the elimination of accumulated toxic metabolites [198]. Microfluidic devices have begun to address these limitations, using pumps to dynamically circulate the culture medium between connected cell chambers to constantly renew nutrients while simultaneously removing toxic metabolites [199].…”

Recent Developments in Inertial and Centrifugal Microfluidic Systems along with the Involved Forces for Cancer Cell Separation: A Review

“…This is where microfluidic devices come in. Microfluidic devices are small, lab-on-a-chip devices that provide a controlled environment for the separation of CTCs and facilitate the prediction of their behaviors, reduce the sample quantity, and minimize the diagnostic time and treatment cost [31][32][33]. It is worth mentioning that traditional clinical procedures can be scaled down to the microscale by mimicking macroscale processes and applying various external forces [34,35].…”